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classification of cryptosporidium species from patients with sporadic cryptosporidiosis by use of sequence-based multilocus analysis following mutation scanning.in the present study, we analyzed genetic variation in cryptosporidium species from humans (n = 62) with clinical cryptosporidiosis in south australia. sequence variation was assessed in regions within the small subunit of nuclear rrna (p-ssu), the 70-kda heat shock protein (p-hsp70), and the 60-kda glycoprotein (p-gp60) genes by employing single-strand conformation polymorphism analysis and sequencing. based on the analyses of p-ssu and p-hsp70, cryptosporidium hominis (n = 38) and cryptosporid ...200818448696
first molecular characterisation of cryptosporidium and giardia from bubalus bubalis (water buffalo) in victoria, australia.we conducted a molecular epidemiological survey of cryptosporidium and giardia from bubalus bubalis (water buffalo) on two extensive farms (450 km apart) in victoria, australia. faecal samples (n=476) were collected from different age groups of water buffalo at two time points (six months apart) and tested using a pcr-based mutation scanning-targeted sequencing-phylogenetic approach, employing markers within the small subunit of ribosomal rna (designated pssu) and triose phosphate isomerase (ptp ...201323886616
molecular-based investigation of cryptosporidium and giardia from animals in water catchments in southeastern australia.there has been no large-scale systematic molecular epidemiological investigation of the waterborne protozoans, cryptosporidium or giardia, in southeastern australia. here, we explored, for the first time, the genetic composition of these genera in faecal samples from animals in nine melbourne water reservoir areas, collected over a period of two-years. we employed pcr-based single-strand conformation polymorphism (sscp) and phylogenetic analyses of loci (pssu and pgp60) in the small subunit (ssu ...201223357792
molecular detection of cryptosporidium cuniculus in rabbits in australia.in the united kingdom, rabbits have been reported to harbour genotypes of cryptosporidium (now recognized as c. cuniculus) identical to those from human patients exhibiting symptoms of cryptosporidiosis. the high density of rabbits in many regions of australia, including both rural and urban as well as natural water catchments areas, and the absence of any information on cryptosporidium from lagomorphs in this country stimulated the present study. we undertook an epidemiological study that genet ...201020691809
a practical and cost-effective mutation scanning-based approach for investigating genetic variation in cryptosporidium.in the present study, we used a mutation scanning-targeted sequencing approach to assess variation in part (pgp60) of the 60 kda glycoprotein (gp60) gene among cryptosporidium samples from humans in victoria, australia. two nuclear ribosomal loci (the small subunit rrna gene and the second internal transcribed spacer) were used to identify the samples as cryptosporidium hominis (n = 74), cryptosporidium parvum (n = 23) or cryptosporidium meleagridis (n = 1). in total, nine distinct pgp60 sequenc ...200717960838
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