detection of cryptosporidium parvum oocysts in environmental water in hokkaido, japan.control of cryptosporidiosis is important in public health. rivers that are polluted with cryptosporidium and drinking water that is treated for drinking water production from polluted rivers could result in the waterborne disease of cryptosporidiosis. we carried out an epidemiological study of natural water supplies in hokkaido, one of the largest dairy prefectures in japan. to detect cryptosporidium oocysts in environmental water, the filtration method was used for 28 samples, which were colle ...200111307921
seasonal change in the number of cryptosporidium parvum oocysts in water samples from the rivers in hokkaido, japan, detected by the ferric sulfate flocculation epidemiological study was carried out in natural water supplies of hokkaido, one of the largest dairy prefectures in japan. to investigate the prevalence of cryptosporidium parvum (c. parvum) oocysts water samples were collected from three rivers in the eastern area of hokkaido from august 1999 to october 2001, and c. parvum oocysts were collected and purified by the ferric sulfate flocculation method. the oocysts were detected using the immunofluorescent assay test (ifat) and 4', 6-diamidino ...200312576717
cryptosporidium infection of cattle in the tokachi district, hokkaido.the prevalence of cryptosporidium infection was examined in 480 healthy cattle (0-39 months old) in the tokachi district in hokkaido during the period from june to september in 2000 and from june to july in 2001. c. parvum oocysts were detected in 6 of 50 cattle (0-2 months old) in 2001; while c. muris was detected in 2 of 56 cattle (6-8 months old) in 2001, in 1 of 15 cattle (9-11 months old) in 2001, in 1 of 88 cattle (15-17 months old) in 2000, in 4 of 89 cattle (18-21 months old) in 2000 and ...200312576718
viability and infectivity of cryptosporidium parvum oocysts detected in river water in hokkaido, japan.the viability and infectivity of cryptosporidium parvum (c. parvum) oocysts, detected in water samples collected from river water in hokkaido, were investigated using severe combined immunodeficient (scid) mice. the water samples collected from september 27 through october 10, 2001 by filtration using cuno cartridge filters were purified and concentrated by the discontinuous centrifugal flotation method. from 1.2 x 10 (5) liters of the raw river water, approximately 2 x 10(4) oocysts were obtain ...200312808210
molecular epidemiological analyses of cryptosporidium parvum virus 1 (cspv1), a symbiotic virus of cryptosporidium parvum, in japan.we show that cryptosporidium parvum virus 1 (cspv1), a member of the family partitiviridae, genus cryspovirus that can infect cryptosporidium parvum, is a new candidate for high-resolution tool for tracing c. parvum. cspv1 was detected in all c. parvum-positive samples tested. phylogenetic analysis of dsrna1 sequence from cspv1 can distinguish infected areas of c. parvum on the national level. sequences detected in samples from iwate prefecture and other islands (tanegashima, and okinawa) belong ...201626439535
molecular characterization of cryptosporidium parvum from two different japanese prefectures, okinawa and hokkaido.infectious diarrhea is the most frequent cause of morbidity and mortality in neonatal calves. cryptosporidium parvum is one of the main pathogens associated with calf diarrhea. although diarrhea is a symptom of infection with various pathogens, investigations to detect the types of pathogens have never been performed in japan. this study investigated the prevalence of four major diarrhea-causing pathogens in calves: c. parvum, rotavirus, coronavirus, and enterotoxigenic escherichia coli (e. coli ...201525481361
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