Publications

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can respiratory syncytial virus and influenza a be distinguished clinically in institutionalized older persons? 19957836643
non-influenza respiratory viruses may overlap and obscure influenza activity.to report the number and timing of influenza a isolates, as well as overlapping respiratory viruses. co-circulating respiratory viruses may obscure the determination of influenza activity.199910484251
mortality following isolation of various respiratory viruses in nursing home residents.to compare mortality following isolation of influenza a to mortality following isolation of other respiratory viruses in a nursing home.199910614604
national disease burden of respiratory viruses detected in children by polymerase chain reaction.the epidemiology of community-acquired respiratory viruses at the children's hospital of wisconsin between 1996 and 1998 was examined with molecular [multiplex (m) pcr] and standard virologic methods.200414730265
cytokine response patterns, exposure to viruses, and respiratory infections in the first year of life.daycare attendance and siblings are associated with viral-induced wheezing in children. preexisting immunologic factors may influence the expression of viral infections in infancy, and in turn, recurrent infections may influence the development of immune responses. a total of 285 children were enrolled in the childhood origins of asthma project at birth and followed for at least 1 year. cord blood and 1-year mononuclear cells were stimulated with phytohemagglutinin, and cytokine-response profile ...200415087299
rapid semiautomated subtyping of influenza virus species during the 2009 swine origin influenza a h1n1 virus epidemic in milwaukee, wisconsin.in the spring of 2009, a novel influenza a (h1n1) virus (swine origin influenza virus [s-oiv]) emerged and began causing a large outbreak of illness in milwaukee, wi. our group at the midwest respiratory virus program laboratory developed a semiautomated real-time multiplex reverse transcription-pcr assay (seasonal), employing the nuclisens easymag system (biomérieux, durham, nc) and a raider thermocycler (handylab inc., ann arbor, mi), that typed influenza a virus, influenza b virus, and respir ...200919641066
development of a rapid automated influenza a, influenza b, and respiratory syncytial virus a/b multiplex real-time rt-pcr assay and its use during the 2009 h1n1 swine-origin influenza virus epidemic in milwaukee, wisconsin.rapid, semiautomated, and fully automated multiplex real-time rt-pcr assays were developed and validated for the detection of influenza (flu) a, flu b, and respiratory syncytial virus (rsv) from nasopharyngeal specimens. the assays can detect human h1n1, h3n2, and swine-origin (s-oiv) h1n1 flu a viruses and were effectively used to distinguish flu a infections (of all subtypes) from flu b and rsv infections during the current s-oiv outbreak in milwaukee, wi. the analytical limits of detection we ...201019959800
whole genome sequencing and evolutionary analysis of human respiratory syncytial virus a and b from milwaukee, wi 1998-2010.respiratory syncytial virus (rsv) is the leading cause of lower respiratory-tract infections in infants and young children worldwide. despite this, only six complete genome sequences of original strains have been previously published, the most recent of which dates back 35 and 26 years for rsv group a and group b respectively.201121998661
use and interpretation of a rapid respiratory syncytial virus antigen detection test among infants hospitalized in a neonatal intensive care unit - wisconsin, march 2015.on march 25, 2015, the wisconsin division of public health was notified of a possible respiratory syncytial virus (rsv) infection outbreak among infants hospitalized in a neonatal intensive care unit (nicu). on march 23, the index patient (neonate a), aged 3 days, had feeding intolerance and apnea. a nasopharyngeal swab specimen collected from neonate a was tested using a single-manufacturer rapid rsv antigen detection test (rradt) at the hospital laboratory; the result was positive. the followi ...201526270063
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