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molecular characterization of an outbreak of respiratory syncytial virus (subgroup a) in havana, cuba, by monoclonal antibodies and restriction mapping (n gene).twenty-one respiratory syncytial virus (rsv) strains isolated from one outbreak in havana, cuba (1994 to 1995), were analyzed to determine their relatedness. all isolated strains were classified as subgroup a by monoclonal antibodies. of 21 rsv strains examined, 20 were classified as having restriction pattern np4 and only 1 was classified as having restriction pattern np5.19979302221
unusual antigenic and genetic characteristics of human respiratory syncytial viruses isolated in cuba.the g protein of 23 strains of human respiratory syncytial virus isolated in havana, cuba, between october 1994 and january 1995 was analyzed at the antigenic and genetic level. all viruses reacted with 10 of 11 antibodies specific for the long strain. moreover, the g protein gene of the cuban isolates had only five nucleotide differences from the sequence of the long gene. the homogeneity of the cuban isolates and their resemblance to an ancient strain, such as long, are at odds with previous f ...19989696858
[serologic study for determining the circulation of respiratory viruses in havana city].during 1991, 2,400 serum samples from subjects under 15 years, and 2,400 serum samples from subjects with the same age or over 15 years were assessed against antigens of 7 respiratory viruses by the complement fixation test or by the haemagglutination inhibition technique. the results from these investigations allowed to determine the little circulation of the respiratory syncytial virus which may result in an increase of a susceptible population and the occurrence of outbreaks. the endemic stat ...19929768216
[determination of complement-fixing antibodies to respiratory syncytial virus. longitudinal study in a population of less than 1 year of age in the city of havana].respiratory syncytial viruses (rsv) are considered among the most important agents causing acute respiratory infections in infants below 1 year of age. a 10-year longitudinal study of monosera from children under 15 years was carried out; 1,069 monosera from children under 1 year were studied using the rsv antigen complement-fixing technique. it was observed that there were short or longer periods between the peaks of positive sera and when they were compared with medical care reports, they show ...19949768240
[subgroups classification of strains of the respiratory syncytial virus isolated in an outbreak in ciudad de la havana].a high number of acute respiratory diseases was detected among children under one year admitted in a hospital of havana city. 25 respiratory syncytial virus strains were obtained from 93 patients studied. viral isolations were multiplied in hep-2 cells and after observing a cytopathic effect of 80%, they were classified into subgroups by the indirect immunofluorescence technique, using anti-protein g antibodies from the respiratory syncytial virus. all the samples studied were classified within ...19969768286
antigenic and genetic characterization of twenty-six strains of human respiratory syncytial virus (subgroup a) isolated during three consecutive outbreaks in havana city, cuba.twenty-six human respiratory syncytial virus strains (subgroup a) isolated from three outbreaks in havana city during the period 1994/95, 1995/96 and 1996/97 were analyzed to determine their antigenic and genetic relationships. analyses were performed by monoclonal antibodies and restriction mapping (n gene) following amplification of the select region of the virus genome by polymerase chain reaction. all isolated strains were classified as subgroup a by monoclonal antibodies and they showed a r ...199910446003
[rapid diagnosis of principal respiratory viruses in the city of havana, 1995-97].acute respiratory diseases (ard) are the most common infections in humans and difficult to prevent. viruses have been recognized as predominant ethiological agents. in cuba, ard constitute a major problem of health and are the first cause of morbidity and important cause of mortality. in this paper, rapid diagnosis was performed to 516 clinical samples which arrived to the reference respiratory viruses laboratory of the pedro kourí institute of tropical medicine (ipk) from different parts of hav ...200010785939
first report on fatal myocarditis associated with adenovirus infection in cuba.myocarditis is caused frequently by viral infections of the myocardium. in the past, enteroviruses (ev) were considered the most common cause of myocarditis in all age groups. other viruses that cause myocarditis are adenovirus and influenza viruses. parvovirus b19 infection is associated sometimes with myocarditis. members of the herpesviridae family, cytomegalovirus (cmv), and human herpesvirus 6 (hhv-6) have been associated occasionally with myocarditis. during an atypical outbreak of acute f ...200818712847
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