comparison of immunofluorescence and immunoperoxidase methods for viral diagnosis at a distance: a who collaborative study.nasopharyngeal secretions collected in newcastle were examined in both newcastle and stockholm for the presence of influenza virus type a and respiratory syncytial (rs) virus by immunofluorescence and immunoperoxidase techniques. a total of 139 specimens were examined in this way and the agreement between the two centres for immunofluorescence was 94% for influenza virus a, 95% for respiratory syncytial virus, and 95% for negative specimens. this technique can therefore be used for examining spe ...1978352565
hospitalization for lower respiratory disease during 20 yrs among under 5 yr old children in stockholm county: a population based survey.lower respiratory disease (lrd) is a common cause of hospitalization in infants, and episodes of obstructive lrd increase the risk for asthma later in life. the purpose of this study was to assess time trends and geographical variation of first time hospitalization for lrd among children in stockholm county, sweden. data on first time admittance for lrd among children aged up to 5 yrs from 1973 through 1992 were obtained from the stockholm county council hospital discharge register, and populati ...19989551740
palivizumab prophylaxis and hospitalization for respiratory syncytial virus disease in the stockholm infant population, 1999 through 2002.there are few independent, population-based reports that estimate the risk of hospitalization of respiratory syncytial virus (rsv)-infected infants before and during the palivizumab era. we present figures from the greater stockholm area during the three seasons after the introduction of palivizumab and relate them to data based on 1400 hospitalizations for rsv disease in the same population area during 1987 through 1998.200414743042
respiratory virus infections in stockholm during seven seasons: a retrospective study of laboratory diagnosis.a retrospective analysis of the virological findings in all respiratory samples (7303) analysed at the laboratory of karolinska hospital between 1993 and 2000 was performed. the findings were studied according to age and seasonal variation, and the methods were evaluated. most samples were from children. rsv was the dominant agent, found in 34% of all samples from children 0-1 y of age. influenza a was found in 13% of samples from the age group 2-5 y. influenza a dominated among adults and the e ...200415307569
childhood encephalitis in sweden: etiology, clinical presentation and outcome.acute encephalitis is a relatively uncommon but potentially harmful cns inflammation usually caused by infection. the diagnosis is difficult to establish and the etiology often remains unclear. furthermore, the long-term prognosis of acute encephalitis in children is poorly described. in this study, we characterize childhood encephalitis from a swedish perspective in regard to etiology, clinical presentation and sequele. we retrospectively studied all children (n=93) who were admitted for acute ...200818313340
genotyping of respiratory syncytial virus (rsv) group a in stockholm, sweden, using pcr and two-dimensional melting curve analysis.genotyping of respiratory syncytial (rs) virus group a, by means of a novel method based on pcr, fret (fluorescence resonance energy transmission) detection and two-dimensional melting curve analysis, was carried out on 80 rs virus samples of group a collected in stockholm from 1976 to 2005. the tm values were assessed for three different genotypes (ga2, ga5 and ga7) circulating in sweden. two pairs of probes were used and results of subsequent data analysis were plotted in a two-dimensional sys ...200818397467
self-sampling for analysis of respiratory viruses in a large-scale epidemiological study in sweden.viral diagnosis of respiratory tract infections has so far required sampling by health professionals,hampering large-scale epidemiological studies of virus-specific disease outcomes. as part of a population-based, prospective study of work-related risk factors for transmission of viral infections (swede-i), we developed a scheme for self-sampling with nasal swabs. random selection from the gainfully employed population of a medium-sized town in central sweden resulted in a study cohort of 2,237 ...201525811646
epidemiologic characteristics and seasonal distribution of human metapneumovirus infections in five epidemic seasons in stockholm, sweden, 2002-2006.the presence of human metapneumovirus (hmpv) was analyzed retrospectively by reverse transcriptase-polymerase chain reaction (rt-pcr) in five epidemic seasons, in stockholm, 2002-2006. the occurrence of hmpv was compared with five common respiratory viruses; respiratory syncytial virus, influenza a virus, influenza b virus, parainfluenza virus and adenovirus. with a detection rate of 2.9% (n = 143/4,989) in nasopharyngeal samples over the whole period, hmpv was the fourth most common respiratory ...200818649344
molecular epidemiology of respiratory syncytial virus (rsv) of group a in stockholm, sweden, between 1965 and 2003.the epidemiology of respiratory syncytial virus (rsv) group a was followed by nucleotide sequencing of the variable parts of the glycoprotein (g) gene. the amino acid sequences of an aminoterminal (a-terminal, amino acids 90-132) and carboxyterminal (c-terminal, amino acids 262-298) portion of the g protein in 47 virus strains, collected in stockholm, between 1965 and 2004, were determined. in phylogenetic analysis jointly with previously described genotypes (ga1 to ga7 and saa1), 33 virus strai ...200415351487
wheezing bronchitis in children. incidence, viral infections, and other risk factors in a defined population.risk factors for severe wheezing bronchitis were studied in children aged 4 months to 4 years in need of hospitalization. the children included in the study consisted of all cases generated from a geographically defined population, the catchment area of st göran's hospital in stockholm. the incidence was 3/1000 children and year, during the two years of observation, with the highest rate in boys under the age of 18 months (4.7/1000). symptoms of a preceding upper respiratory tract infection were ...19968792378
molecular epidemiology and genetic variability of respiratory syncytial virus (rsv) in stockholm, 2002-2003.the epidemiology and genetic variability of circulating respiratory syncytial virus (rsv) strains in stockholm during the season 2002-2003 were studied in consecutive rsv isolates derived from respiratory samples and diagnosed in the laboratory. two hundred thirty-four viruses were sequenced. the samples were mainly from children under 1 year old (79%). the phylogeny of the n-terminal part of the g gene was studied after amplification and sequencing. one hundred fifty-two viruses belonged to sub ...200818041002
clara cell protein 10 polymorphism is not associated with severe respiratory syncytial virus infection.aim: to investigate a possible correlation between severe respiratory syncytial virus (rsv) infection and single-nucleotide polymorphism in the clara cell protein 10 (cc10) gene (a38g). methods: children hospitalized with severe rsv lower respiratory tract (ltri) infection at karolinska university hospital stockholm during three subsequent rsv seasons were selected and genotyped. age-matched controls were used. the two groups were compared regarding single nucleotide polymorphism in the cc10 g ...201121767304
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