identity of ehrlichial dna sequences derived from ixodes ricinus ticks with those obtained from patients with human granulocytic ehrlichiosis in slovenia. | adult ixodes ricinus (acari: ixodidae) ticks collected near ljubljana, slovenia, were tested for the agent of human granulocytic ehrlichiosis (hge) by using pcr assays based on the 16s rrna gene. three (3.2%) of 93 ticks were found to contain granulocytic ehrlichiae. nucleotide sequences of portions of the bacterial groesl heat shock operon amplified from these ticks were identical or nearly (99.8%) identical to those previously determined for human patients with hge from slovenia, providing add ... | 1999 | 9854093 |
infection rate of ixodes ricinus ticks with borrelia afzelii, borrelia garinii, and borrelia burgdorferi sensu stricto in slovenia. | in spring 1993, ixodes ricinus ticks were collected from six regions of slovenia to determine their overall rate of infection with borrelia burgdorferi sensu lato and to assess the frequency of individual species in these tick populations. ticks were dissected and midgut tissue inoculated into modified barbour-stoenner-kelly (bsk ii) medium. borrelia isolates were differentiated into separate species using species-specific polymerase chain reaction (pcr) primers and by large restriction fragment ... | 1995 | 8654451 |