lyme disease in italy, 1983-1996. | this paper is a brief review of the epidemiology of lyme disease in italy. the first case of the illness was identified by crovato in liguria in 1983. in the following years, many other cases have been reported from all italian regions with the exception of valle d'aosta, basilicata and calabria. the exact number of cases in our country is not known because lyme disease was not a notifiable disease until 1990, but on the basis of literature data, at least 1324 cases have been observed in the fou ... | 1998 | 9812324 |
detection of hge agent-like ehrlichia in ixodes ricinus ticks in northern italy by pcr. | little is known about the distribution of human granulocytic ehrlichiosis (hge) in europe and even less is known in italy, where no case of clinically documented hge has been reported. in a previous study we reported the presence of ehrlichia dna in ixodes ricinus ticks from central italy. by the use of an ehrlichia-specific pcr we found that 24% of the ticks were positive. furthermore, we demonstrated a simultaneous coinfection of the same tick by both, borrelia burgdorferi and ehrlichia phagoc ... | 1998 | 10048173 |
tick-borne encephalitis virus in northern italy: molecular analysis, relationships with density and seasonal dynamics of ixodes ricinus. | ixodes ricinus ticks were collected from dragging vegetation and from shot roe deer in the province of trento and belluno in northern italy. ticks were pooled for analyses and from 1060 pools of ticks collected in the province of belluno and 12390 tick samples collected in trentino, four proved positive by immunofluorescence microscopy using a tick-borne encephalitis (tbe)-specific antiserum. the identity of the virus isolates was determined by rt-pcr cycle sequencing and they were all found to ... | 2001 | 11583449 |
molecular identification of borrelia valaisiana and hge-like ehrlichia in ixodes ricinus ticks sampled in north-eastern italy: first report in veneto region. | pcr amplification was applied to screen the presence of both borrelia burgdorferi s.l. and ehrlichia species in pools of field-collected ixodes ricinus ticks. the specimens so far analysed (n = 55), grouped in 11 pools, were sampled in feltre area (veneto region, ne italy). five pools proved positive for b. valaisiana (45%) and one of them (9%) was also positive for ehrlichia, that was further characterised as a hge-like ehrlichia. this is the first report of the two bacteria in the veneto regio ... | 2001 | 11921543 |
bartonella henselae in ixodes ricinus ticks (acari: ixodida) removed from humans, belluno province, italy. | the potential role of ticks as vectors of bartonella species has recently been suggested. in this study, we investigated the presence of bartonella species in 271 ticks removed from humans in belluno province, italy. by using primers derived from the 60-kda heat shock protein gene sequences, bartonella dna was amplified and sequenced from four ixodes ricinus ticks (1.48%). to confirm this finding, we performed amplification and partial sequencing of the pap31 protein and the cell division protei ... | 2003 | 12643827 |
genetic diversity of bacterial agents detected in ticks removed from asymptomatic patients in northeastern italy. | a total of 360 ticks were removed from 353 asymptomatic subjects in belluno province, italy and surrounding areas, from 1998 to 2001. ticks were identified as ixodes ricinus (357), ixodes hexagonus (1), rhipicephalus sanguineus (1), and ixodes ventalloi (1). tick dna was investigated by pcr and subsequent sequencing of amplified products to identity associated bacterial agents. primers targeting different genes of rickettsia (glta and ompa), borrelia (16s rdna, rpob), francisella (16s rdna), and ... | 2003 | 12860623 |
distribution of 'ehrlichia walkeri' in ixodes ricinus (acari: ixodidae) from the northern part of italy. | the prevalence of 'ehrlichia walkeri' in ixodes ricinus in northern italy was studied. a total of 456 ticks were collected from seven places around belluno, and 292 living ticks were examined using polymerase chain reaction (pcr). nineteen ticks were positive for glta of 'e. walkeri', and the prevalence rate for each place ranged from 0 to 12.8%. the disparity of prevalence rates among areas examined suggests that 'e. walkeri' is not widespread in ticks and requires an animal host for its mainte ... | 2005 | 15691013 |
a study of the presence of b. burgdorferi, anaplasma (previously ehrlichia) phagocytophilum, rickettsia, and babesia in ixodes ricinus collected within the territory of belluno, italy. | in the years 2000 and 2001, we sampled ticks in order to establish the distribution of ixodes ricinus in the province of belluno; 5987 tick samples from 244 sites throughout the province were gathered, by dragging for a 5-min period. in 40 sites, seasonal variations and cycle stages of the parasites were studied at monthly intervals from march to september. a polymerase chain reaction (pcr) technique was used to identify the tick-infected sites. of 1931 individual ticks, 8.23% were positive for ... | 2006 | 16584324 |
first detection of tbe virus sequences in ixodes ricinus from friuli venezia giulia (italy). | we report, for the first time, the presence of tick-borne encephalitis (tbe) virus in the tick ixodes ricinus collected in the friuli venezia giulia region of north-eastern italy. using molecular methods, we demonstrate that the tbe virus carried by ticks from fvg is a western european strain. sequence analysis of the 5' ncr showed 98.4% identity to the neudoerfl strain. | 2006 | 16841557 |
detection and identification of rickettsia species in the northeast of italy. | this study was carried out using ixodes ricinus ticks collected during 2005 and 2006 from the friuli venezia giulia (fvg) region in the northeastern part of italy and an area along the slovenian side of the western border of italy. the results indicate that rickettsia spp. is widely distributed throughout these areas, with the greatest prevalence in the central part of the fvg region. the prevalence of rickettsia spp. was 4.5% during 2005 and 6.1% during 2006. by sequencing the 16s rrna gene, we ... | 2008 | 18637720 |