| isolation and amplification by polymerase chain reaction dna of babesia microti and babesia divergens in ticks in poland. | babesia microti and b. divergens, the etiological agents of human babesiosis, are transmitted by the bite of ixodes ricinus. the purpose of this study was differentiation of those two species in ticks collected in urban woods in the city szczecin (north-western poland). the prevalence of the dna of babesia were investigated by pcr amplification with primers to the fragment from a gene encoding the nuclear small-subunit ribosomal rna (ss-rdna). we examined a total of 533 specimens of ixodes ricin ... | 2001 | 11748876 |
| coexistence dna of borrelia burgdorferi sensu lato and babesia microti in ixodes ricinus ticks from north-western poland. | the tick ixodes ricinus may carry microorganisms which cause serious human and animal diseases, i.a., the lyme disease (borreliosis), caused by the spirochaete borrelia burgdorferi sensu lato and babesiosis, induced by the protozoan babesia microti. both microbe species may co-occur in the same and other species of the genus tick and produce a mixed infection in humans and animals. the major objective of the study was to identify dna of b. burgdorferi and b. microti in the i. ricinus ticks colle ... | 2002 | 12088393 |
| the prevalence of spirochete borrelia burgdorferi sensu lato in ticks ixodes ricinus and mosquitoes aedes spp. within a selected recreational area in the city of szczecin. | the aim of this study was to determine the prevalence of spirochete borrelia burgdorferi s.l. in ticks ixodes ricinus and mosquitoes aedes spp. within the bukowa forest, collected between 2000 and 2001. the study covered 215 ticks (193 nymphs and 22 adults) and 947 mosquitoes female of the genus aedes. spirochetes of borrelia burgdorferi s.l. were detected in the arthropods studied with the method of indirect immunofluorescence assay (ifa). positive readings of the immunological reaction were st ... | 2004 | 15236506 |
| [anaplasma phagocytophila and protozoans of babesia genus in dogs from endemic areas of lyme disease in north-western poland]. | infections caused by the spirochete borrelia burgdorferi sensu lato may be accompanied by other microorganisms, such as anaplasma, ehrlichia and babesia. these pathogens are transmitted by the ticks and are a risk to humans and animals. ixodes ricinus ticks collected from recreational areas of szczecin and northwestern poland contained dna of the pathogens mentioned above and cases of double and triple coinfection have been documented. the aim of this paper was to determine if dogs suspect to ti ... | 2004 | 16865968 |
| [isolation of borrelia burgdorferi sensu lato in ticks ixodes ricinus by polymerase chain reaction (pcr)]. | attempts were made to identify the causative orgamsm of lyme disease in szczecin from tick ixodes ricinus as a vector. ticks were collected in 1997 year in forest areas of szczecin, from localites associated with numerous attendance of people. the method used in this study was the polymerase chain reaction (pcr) on the flagellin structural gene fla of borrelia burgdorferi sensu stricto. the flagellin pcr primer set reaction was conservative for b. burgdorferi sensu stricto, b. afzelii and b. gar ... | 2000 | 16886357 |
| [the occurrence of ixodes ricinus in the select recreative areas in the province of szczecin. part i]. | among polish ticks species the most common ixodes ricinus has the biggest medical importance. within the last few years, the incidence of disease transmitted by ticks has rapidly increased. we have made a thorough analysis of the quantative and rate per cent of occurrence of various stages ixodes ricinus in the forest areas of some places in szczecin province and in the parks of szczecin, that are known as highly recreative and frequently visted by many people. a total of 426 (68% numphs) specim ... | 1999 | 16888993 |
| [seasonal activity of the ticks ixodes ricinus in the lakesides and forests habitats of szczecin and environs and its infestation with the spirochete borrelia burgdorferi]. | the article presents the results of researche (1999-2000) on numbers and seasonal activity of ixodes ricinus in selected lakesides and forests habitats of szczecin and on occurence of the spirochetes borrelia burgdorferi sensu lato in ticks. a total of 8065 ticks were collected from 6 localities : 647 larvae, 4549 nymphs, 1624 females and 1244 males. the spirochetes b. burgdorferi were found (using the pcr techniques) in 2271 specimens e.c. 28.1% whole population. numbers infected larvae were 21 ... | 2001 | 16894752 |
| ticks and mosquitoes as vectors of borrelia burgdorferi s. l. in the forested areas of szczecin. | the aim of the study was to determine the infection level of adult forms and larvae of ticks and mosquitoes with borrelia burgdorferi in the forested areas of szczecin. a total of 1699 ticks ixodes ricinus, including 1422 nymphs, 277 adult forms and 2862 mosquito females representing the genera aedes (89.6%) and culex (10.4%) were collected between the years 2004 and 2005. a further 3746 larvae and 1596 pupae of culex pipiens pipiens were colleted from water bodies. borrelia burgdorferi s. l. wa ... | 2007 | 18274258 |
| preliminary study on the occurrence of toxoplasma gondii in ixodes ricinus ticks from north-western poland with the use of pcr. | a batch of 259 ixodes ricinus ticks collected by flagging in woodlands of north-western poland (szczecin area) was examined for the presence of toxoplasma gondii dna with nested polymerase chain reaction (pcr). positive isolates were genotyped with the restriction fragment length polymorphism (rflp) analysis of b1 gene marker. another batch of 340 i. ricinus ticks from szczecin area was homogenized and inoculated into mice. subsequently, the biological material obtained from inoculated mice was ... | 2008 | 19061272 |
| wild game as a reservoir of anaplasma phagocytophilum in north-western poland. | the aim of the study was to determine the role of game animals as reservoirs of anaplasma phagocytophilum, a bacteria species transmitted by ixodes ricinus ticks, from north-western poland (zachodniopomorskie vovoidship). the area under question is endemic for a. phagocytophilum. | 2007 | 17912805 |
| [prevelance of the etiological agent of human ehrlichiosis (hge) in ticks from west-north poland]. | ehrlichias occur in ticks in the cells of their haemolymph-hematocytes. they enter the vertebrate host organism with the saliva of the tick, during a blood meal. humans can also be the hosts for this pathogen. two pathogens cause a humane disease-monocytic ehrlichiasis (e. chaffensis) or granulocytic ehrlichiasis (hge factor). the above disease units are difficult to diagnose because of their non-specific symptoms. a preliminary study has been conducted on the prevalence of the hge factor in the ... | 2001 | 16888958 |
| [the occurrence of ixodes ricinus in the selected recreative areas in the province of szczecin. part iii]. | within the last years, the incidences of diseases transmitted by ixodes ricinus tick have rapidly increased. we estimated the occurrence of ixodes ricinus in the popular recreation urban areas in szczecin and in the province of szczecin. the study was carried out in 1999, with two samples at each site, and were compared with data of 1998. the temperature and humidity of air were measured. the most ticks were found in the range of 70-80% relative humidity of air. among 3.198 specimens collected 5 ... | 2002 | 16888942 |
| [the occurrence of ixodes ricinus in the selected recreative areas in the province of szczecin. part ii]. | the aim of the study was to estimate the occurrence (the quantative and rate percent) of ixodes ricinus in the popular recreation areas in szczecin (arkonka, osów, głebokie, landscape park of szczecin, dabie forest park, zdroje forest park) and in province of szczecin (forest of goleniów, ińsko, pobierowo, chojna). investigations were performed in 1998 year, twice on each places; in may/june and repeted in september/october. the temperature and humidity of air were measured. obtained specimens w ... | 2000 | 16886345 |
| molecular identification of babesia parasites isolated from ixodes ricinus ticks collected in northwestern poland. | in the present study, pcr has been applied to detect and analyze dna of babesia spp. extracted from ixodes ricinus ticks. collection of i. ricinus was made in 6 forested areas of zachodniopomorskie voivodship, poland, during 2 seasonal peaks of tick activity, i.e., spring and autumn, 2001. in total, 1,328 i. ricinus were collected and processed for pcr with f34 and r323 primers. babesia spp. was detected in 28 (2% of 1,328 tested) ticks; 26 were identified as b. divergens. the other 2 were ident ... | 2006 | 16629311 |
| zoonotic reservoir of babesia microti in poland. | babesiosis is as one of the emerging human and animal diseases transmitted by ticks. it is caused intraerythrocytic parasites of the genus babesia. current evidence of human babesiosis suggests that the majority of cases are involved by babesia divergens and babesia microti piroplasms. as zoonotic reservoir of b. microti serve small mammals--insectivores and rodents. the occurrence of this parasite in natural environment in poland is documented for various regions, in the wide range of mammal ho ... | 2004 | 15787199 |
| [occurrence of genomic species of borrelia burgdorferi sensu lato in populations of ixodes ricinus ticks from west pomerania]. | the aim of this study was to determine the incidence of borrelia burgdorferi sensu lato infections in ixodes ricinus ticks from recreational areas of the province of west pomerania. ticks (nymphs and adults) were collected between april and october 2000 and 2001 from 10 sampling sites. the presence of the spirochete, borrelia burgdorferi sensu lato, was confirmed with an indirect fluorescence assay (ifa) and the polymerase chain reaction (pcr). in addition, a nested pcr technique was used to det ... | 2002 | 14601491 |
| the occurrence of borrelia burgdorferi sensu lato in the populations of ixodes ricinus in forest areas of szczecin during 2000-2001. | the aim of the present study was to determine the seasonal abundance of ticks, ixodes ricinus and their infection frequency with spirochetes, borrelia burgdorferi in wooded areas of the vicinity of the city of szczecin during 2000-2001. the ticks were collected from april to october. presence of borrelia burgdorferi sensu lato was detected with the method of indirect immunofluorescence (ifa). the results showed differences in seasonal activity of ticks within the years of the study. in the year ... | 2003 | 12852727 |