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preliminary studies on the relationship between ixodes ricinus activity and tick-borne infection among occupationally-exposed inhabitants of eastern poland.relative density (activity) of the tick ixodes ricinus was determined in five districts of the lublin region (eastern poland) by vegetation flagging. tick activity values were compared to the determined by elisa tests seroprevalence to tick-borne encephalitis virus (tbev) and borrelia burgdorferi in forestry workers and farmers from the same areas. a significant correlation between tick activity and seroprevalence to both tbev and b. burgdorferi antigen was found in farmers and in the total exam ...200111748891
study on the occurrence of borrelia burgdorferi sensu lato and tick-borne encephalitis virus (tbev) in ticks collected in lublin region (eastern poland).in 114 ixodes ricinus ticks from 1 district of lublin region (eastern poland) examined by dark field microscopy method, the presence of motile spirochetes, morphologically corresponding to borrelia species was detected in 8.8% of the total examined ticks. the highest infection rate was noted among females (16.7%), much lower in males (7.1%) and nymphs (4.0%). examination of 550 ticks collected from 3 districts of the lublin region by polymerase chain reaction (pcr) showed that 5.3% of the total ...200212088406
[evaluation of occurrence of spirochetes borrelia burgdorferi sensu lato in ixodes ricinus ticks in selected areas of the lublin region by polymerase chain reaction method (pcr)].during the period 2001-2002, 1098 ixodes ricinus ticks were collected at forest sampling sites and the degree of their infection with borrelia burgdorferi spirochetes was determined by means of polimerase chain reaction (pcr). the presence of borrelia burgdorferi genetic material was noted in 69 cases (6.3%). it was confirmed that the frequency of infection of adult forms of ticks (males and females) was nearly twice as high as nymphs. the highest degree of infection was observed in females--9.5 ...200316889023
prevalence of borrelia burgdorferi genospecies in ixodes ricinus ticks from lublin region (eastern poland).the objective of the study was to determine the prevalence rate of 3 borrelia burgdorferi genospecies in ixodes ricinus ticks collected from wooded areas of the lublin region (eastern poland). a group of 1,813 i. ricinus ticks from 6 districts were examined for the presence of borrelia burgdorferi sensu lato (b.b.s.l.) by polymerase chain reaction (pcr). another group of 438 i. ricinus ticks collected from 4 districts were examined for the presence of b.b.s.l. by culture on bskh liquid medium co ...200617196005
prevalence of babesia microti in ixodes ricinus ticks from lublin region (eastern poland).a total of 1,367 ixodes ricinus ticks collected from 5 districts of the lublin region (eastern poland) were examined for the presence of babesia microti dna by pcr and nested-pcr. as many as 74 ticks (5.4 %) were found to be infected with babesia microti. the infection rate varied significantly with stage/sex of ticks (chi (2) =16.48543, df=2, p < 0.000264). the infection rates in females and males amounted to 6.4 % and 8.8 % respectively and were significantly higher (p=0.006 and p=0.0001 respe ...200617196008
[occurence of the common tick ixodes ricinus l. in environments of various degree and character of anthropogenic impact].material and methods: the study of the occurence and relative numbers of the common tick ixodes ricinus in environments of various degree and character of anthropogenic impact was conducted in 3 forest complexes situated in the lubelskie voivodeship: the gułowskie forests (district of luków), the kozłowieckie forests (district of lubartów) and the dabrowa forest (within the municipal boundaries of lublin). the adopted method was flagging in the period of peak activity of ticks in the springs of ...200717912809
prevalence of anaplasma phagocytophilum in ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16s rrna and anka genes.a total of 684 ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by flagging lower vegetation in 6 forest districts located on the territory of lublin province (eastern poland). ticks were examined by polymerase chain reaction (pcr) method for the presence of anaplasma phagocytophilum dna with two pairs of primers: ehr521/ehr747 for detecting 16s rrna gene, and la6/la1 for detecting anka gene. to study the relationship between infection in ticks and people occupational ...200718247465
[habitat preferences of the common tick ixodes ricinus l. in lublin region].a study was conducted on habitat preferences of the common tick ixodes ricinus. material and methods: eleven habitats were distinguished within the administrative borders of the city of lublin as well as in the gułowskie forests and the kozłowieckie forests (lublin voivodeship). they included meadows and pastures, thickets, poletimber woods, dry pine forests, mesic pine forests, mixed forests, mixed woods, deciduous woods, city parks, suburban wooded areas, and wasteland within the administrativ ...200818702316
study on lyme borreliosis focus in the lublin region (eastern poland).a suburban focus of lyme borreliosis situated 11 km from the southern border of the city of lublin (eastern poland) was characterized. the focus covers an area of circa 100 km(2), surrounding 3 localities inhabited by circa 7,500 people engaged mostly in farming. it was demonstrated that on the area of focus the infection rate of ixodes ricinus ticks with borrelia burgdorferi, frequency of serological response of inhabitants to the antigen of borrelia burgdorferi, and incidence of lyme borrelios ...200819061271
the occurrence of toxoplasma gondii and borrelia burgdorferi sensu lato in ixodes ricinus ticks from east poland with the use of pcr.715 ixodes ricinus ticks from 4 regions of lublin macroregion (piotrowice, polesie national park, dabrowa and leczynsko-wlodawskie lakeland) were examined for the presence of borrelia burgdorferi sensu lato and toxoplasma gondi, using polymerase chain reaction (pcr) and nested-pcr methods. the clonal type of samples tested positive for t. gondii was identified by amplification and fragmenting with restrictions enzymes (rflp-restriction fragment length polymorphism). positive results were confirm ...200920047269
prevalence of tick-borne encephalitis virus in ixodes ricinus and dermacentor reticulatus ticks collected from the lublin region (eastern poland).a total of 875 nymphal and adult ixodes ricinus ticks and 148 adult dermacentor reticulatus ticks were collected by flagging lower vegetation in the lublin region (eastern poland) and examined for the presence of rna of tick-borne encephalitis virus (tbev) by nested rt-pcr. the minimum infection rate of i. ricinus ticks with tbev amounted to 1.6% while the infection rate of d. reticulatus ticks was 10.8%. the results suggest that d. reticulatus may be a potential vector of tbev in central europe ...201121771532
tick bites on humans in the agricultural and recreational areas in south-eastern poland.the investigations were conducted in the lublin province (south-eastern poland) in areas of high agricultural and recreational value. among the 418 patients admitted to medical clinics due to arthropod bites in the years 2003-2005, 184 people (44%) had been bitten by ticks. as shown by the research, high-risk groups include people whose stay in tick habitats is connected with their occupational work (54.5%) as well as recreation and tourism (45.5%). as many as 78.7% of the patients were attacked ...201121736280
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