molecular diversity of campylobacter coli and c. jejuni isolated from pigs at slaughter by flaa-rflp analysis and ribotyping.a population of porcine isolates of camplobacter jejuni (n = 11) and c. coli (n = 17) were examined for genotypic relatedness employing ribotyping, as well as polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis of the flagellin (fla)a gene locus. pcr was employed to amplify a 533 bp fragment from the flaa gene, including the previously described short variable region (svr), employing the novel primers, a2 and al and successfully generated this amplicon for all ...200212449248
comparison of human and porcine strains of campylobacter coli. 200312930063
demonstration of the shorter flagellin (flaa) gene of urease-positive thermophilic campylobacter isolated from the natural environment in northern ireland.the pcr amplicons (about 1450 bp in length) of flaa gene fragments of 11 isolates of urease-positive thermophilic campylobacter (uptc) isolated from the natural environment not including wild birds in northern ireland were demonstrated to be shorter than those of c. jejuni 81116 and six isolates of c. jejuni and c. coli (about 1700 bp) isolated in northern ireland and japan. when the nucleotide lengths of the possible open reading frame (orf) of the flaa genes were determined, those from the 11 ...200617004648
prevalence of campylobacter spp. in raw retail poultry on sale in northern ireland.a year-long survey of fresh, retail poultry products on sale in northern ireland was undertaken to define the prevalence of campylobacter spp. by using protocols based on iso (standard) 10272-1:2006. incubation at 37 and 42 degrees c was undertaken to increase the diversity of isolates obtained. overall, 652 isolates were identified as campylobacter spp. by using pcr and amplified fragment length polymorphic typing. phenotyping wrongly identified 21% of isolates. prevalences of campylobacter fou ...200919777882
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