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[an outbreak of human streptococcus suis serotype 2 infections presenting with toxic shock syndrome in sichuan, china].in mid-july 2005, five patients presented with septic shock to a hospital in ziyang city in sichuan, china, to identify the etiology of the unknown reason disease, an epidemiological, clinical, and laboratory study were conducted.200616792880
[antibody detection and sequencing analysis of hepatitis e virus in human population, swine and chicken in sichuan region in china].to investigate the seroprevalence of hev infection and genotype.200819544648
streptococcus suis sequence type 7 outbreak, sichuan, china.an outbreak of streptococcus suis serotype 2 emerged in the summer of 2005 in sichuan province, and sporadic infections occurred in 4 additional provinces of china. in total, 99 s. suis strains were isolated and analyzed in this study: 88 isolates from human patients and 11 from diseased pigs. we defined 98 of 99 isolates as pulse type i by using pulsed-field gel electrophoresis analysis of smai-digested chromosomal dna. furthermore, multilocus sequence typing classified 97 of 98 members of the ...200616965698
human streptococcus suis outbreak, sichuan, china.from mid-july to the end of august 2005, a total of 215 cases of human streptococcus suis infections, 66 of which were laboratory confirmed, were reported in sichuan, china. all infections occurred in backyard farmers who were directly exposed to infection during the slaughtering process of pigs that had died of unknown causes or been killed for food because they were ill. sixty-one (28%) of the farmers had streptococcal toxic shock syndrome; 38 (62%) of them died. the other illnesses reported w ...200616707046
diet, activity, and other lifestyle risk factors for forearm fracture in postmenopausal women in china: a case-control study.forearm fractures are a major cause of disability in postmenopausal women, but the risk factors have not been previously studied in china and the results from western studies may not be applicable to the chinese population. the aim of this study was to determine whether calcium intake, activity level, lifestyle, and other factors are associated with risk of forearm fracture among chinese postmenopausal women.200616607105
streptococcal toxic shock syndrome caused by streptococcus suis serotype 2.streptococcus suis serotype 2 (s. suis 2, ss2) is a major zoonotic pathogen that causes only sporadic cases of meningitis and sepsis in humans. most if not all cases of streptococcal toxic shock syndrome (stss) that have been well-documented to date were associated with the non-ss2 group a streptococcus (gas). however, a recent large-scale outbreak of ss2 in sichuan province, china, appeared to be caused by more invasive deep-tissue infection with stss, characterized by acute high fever, vascula ...200616584289
[matched case-control study for risk factors of human streptococcus suis infection in sichuan province, china].to study the potential risk factors of human infecting with streptococcus suis.200516471206
the epidemiology of human trichinellosis in china during 2000-2003.the endemic foci of trichinellosis continue to be mainly located in the southwestern, the central and northeastern china. the seroepidemiological surveys of t. spiralis infection in humans were carried out in six provinces or municipals (p/m) of china during 2000-2003, the overall seroprevalence was 3.57%. from 2000 to 2003, 17 outbreaks of human trichinellosis, with 828 cases and 11 deaths, were recorded in 8 p/a of china. all of 11 deaths occurred in the southwestern china (1 case in sichuan, ...200616414006
[the genetic diversity of the second exon about sla-dqb in some chinese native pigs].polymorphism of the second exon about sla-dqb genes in some chinese native pigs was investigated employing pcr-sscp analysis and dna cloning sequencing. combined with the sequences in genbank, 73 new alleles were gained, among them function alleles of sla-dqb were sixty-eight, five alleles of pseudogene (sla-dxb) were gained. there were disequilibrium distribution of the alleles in all breeds, the same allele was shared by most breeds. c08 allele existed in 11 breeds of six groups and in the wil ...200515843340
[polymorphisms and species specificity of d2s2944 and d1s2134 loci in chinese han population in chengdu].to obtain the data of polymorphism distribution of the two str loci d2s2944, d1s2134 in chinese han population in chengdu and evaluate their usefulness in the field of forensic science.200415573761
the origin and genetic differentiation of native breeds of pigs in southwest china: an approach from mitochondrial dna polymorphism.mitochondrial dna (mtdna) of six breeds of native domestic pigs from yunnan province, southwest china, and two wild boars obtained from sichuan, china, and vietnam was analyzed using 20 restriction endonucleases that recognize six nucleotides. restriction maps were made by double-digestion methods and polymorphic sites were located on the map. according to their mtdna restriction types, all the breeds were classified into six groups. genetic distances among groups were calculated to define their ...19938097084
[surveillance of antibodies to human influenza a and b virus in swine population in different seasons in chengdu]. 19883198101
[a serological survey of legionnaires' disease in domestic fowls and animals in chengdu area].a serological survey of antibodies against legionella pneumophila (lp) serotypes in chengdu area by microagglutination test showed that there were high levels of antibodies against serotypes lp1 and lp6 in healthy domestic fowls and animals (rabbits, pigs, chickens, ducks and geese). in rabbits, antibodies against serotypes lp1-lp6 were determined, showing positive rates (titer greater than or equal to 1:16) ranging 6.3-23.8%. among them only the serotype lp3 did not show any positive one. the h ...19892630424
molecular investigation of torque teno sus virus in geographically distinct porcine breeding herds of sichuan, china.torque teno sus virus (ttsuv), infecting domestic swine and wild boar, is a non-enveloped virus with a circular, single-stranded dna genome. which has been classified into the genera iotatorquevirus (ttsuv1) and kappatorquevirus (ttsuv2) of the family anelloviridae. a molecular study was conducted to detect evidence of a phylogenic relationship between these two porcine ttsuv genogroups from the sera of 244 infected pigs located in 21 subordinate prefectures and/or cities of sichuan.201323705989
the role of toll-like receptors in the pathogenesis of streptococcus suis.streptococcus suis is an important agent of swine and human meningitis. sequence type (st) 7 emerged in china and was responsible for the human epidemic caused by s. suis in 2005. the virulence of s. suis st7 is greater than the wild type pathogenic s. suis, st1; however, the mechanisms for this increased pathogenicity are unknown. the aim of this study was to determine the role of different toll-like receptors (tlrs) involved in regulating the host response to the s. suis infection and to specu ...201122055206
[hemagglutinin gene of influenza a (h3n2) virus from human samples in the 2009 influenza epidemic in chengdu].to study the antigenic and genetic characteristics of influenza a (h3n2) virus in the 2009 influenza epidemics in chengdu.201121500568
the epidemiology of human trichinellosis in china during 2004-2009.the endemic foci of human trichinellosis are mainly located in southwestern china. seroepidemiological surveys of trichinella spiralis infection in humans were carried out in 10 out of 34 provinces/autonomous regions/municipals (p/a/m) of china during 2004-2009. the overall seroprevalence was 3.19% (3198/100,282). the highest seroprevalences were mainly located in western china: 8.43% in yunnan, 6.37% in inner mongolia and 5.35% in sichuan. the seroprevalence of trichinella infection in humans w ...201121324302
nitrogen mass flow in china's animal production system and environmental implications.china's economic boom in recent decades has stimulated consumer demand for animal products and consequently, a vast expansion in animal production. from 1978 to 2006, the number of animals increased by 322% for pigs, 209% for poultry, and 2770% for dairy cattle. the objective of the present study was to quantify nitrogen mass flow in china's animal production system at the national scale and to elucidate potential environmental implications. a comprehensive analysis was performed combining stati ...201021043259
streptococcus suis outbreak investigation using multiple-locus variable tandem repeat number analysis.two outbreaks of streptococcus suis st7 occurred in humans in 1998 and 2005 in china. pfge of chromosome restriction fragments found all st7 isolates to be indistinguishable. due to the genetic homogeneity of st7 isolates, development of a rapid sub-typing method with high discriminatory power for st7 isolates is required. in this study, a novel method, mlva, was developed to type s. suis serotype 2 strains. further, this method was used to analyze outbreak-associated st7 strains in china. a tot ...201020618684
unexpected sequence types in livestock associated methicillin-resistant staphylococcus aureus (mrsa): mrsa st9 and a single locus variant of st9 in pig farming in china.in october 2008 nine farrow-to-finish pig farms were visited in shuangliu county in sichuan province, china. one farm was empty for one month but not cleaned after depopulation. dust samples were collected at each farm and analysed for the presence of methicillin-resistant staphylococcus aureus (mrsa). dust samples from four farms were also analysed for the presence of methicillin-susceptible s. aureus (mssa). on 5/9 farms mrsa was isolated and on 2/4 farms mssa was isolated. on two farms, inclu ...200919608357
phylogenetic analysis of mycoplasma suis isolates based on 16s rrna gene sequence in china.to perform phylogenetic analysis of mycoplasma suis isolates derived from china to define the nature of this pathogen, nearly complete of 16s rrna genes from chongqing, sichuan, henan and guangdong isolates were amplified by pcr and sequenced. the four sequences from the blood samples in this study, with other 17 hemoplasmas sequences and related 3 mycoplasma sequences available in the genbank, were aligned using clustal x (version 1.83) sequences alignment program. maximum parsimony, neighbor-j ...200919590972
detection of ctx-m-15, ctx-m-22, and shv-2 extended-spectrum beta-lactamases (esbls) in escherichia coli fecal-sample isolates from pig farms in china.the aim of the present study was to investigate the antibiotic resistance profiles and the molecular epidemiology of extended-spectrum beta-lactamase (esbl)-producing escherichia coli isolates from two production swine operations in sichuan province, china, between august 2002 and february 2007. the prevalence of esbl-producing e. coli increased dramatically from 2.2% to 10.7% during this period. this increase appeared mostly related to dissemination of ctx-m-type esbls among e. coli isolates. o ...200919272004
[mlst typing of streptococcus suis isolated from clinical patients in guangdong province in 2005].objective: intensive surveillance of human s.suis infection was carried out in july and august of 2005 in guangdong province, which coincided with the sichuan outbreak. five isolated cases of human infections were identified during this period, from which 5 s. suis serotype 2 isolates were recovered. mlst analysis showed that these 5 isolates shared identical sequences of 6 mlst housekeeping genes except for one point mutation found within the thra gene fragment, a neutral mutation (tta to ttg) ...200818753081
[allele frequencies and species specificity of five short tandem repeat loci of chinese han population in chengdu].to obtain the data in polymorphism distribution of the five short tandem repeat (str) loci: d18s979, d11s2014, d18s548, d1s1667 and gata164f07 of chinese han population in chengdu, and to evaluate their usefulness in the field of species specificity in forensic science.200617160956
[isolation and identification of streptococcus suis serotype 2 from sick-pig samples of sichuan province].streptococcus suis serotype 2 (ss2) is a major pathogen frequently associated with infections in pigs. there are presently 35 serotypes of s.suis (serotype 1 to 34 and serotype 1/2) recognized on the basis of capsular antigens. few people were reported to infect with ss2 in the past years. however, an accidental case happened in sichuan province of china in 2005. some people got ill and died, and all of them were closely contacted with sick pigs. based on clinical features and epidemiologic data ...200617037069
mitochondrial dna evidence indicates the local origin of domestic pigs in the upstream region of the yangtze river.previous studies have indicated two main domestic pig dispersal routes in east asia: one is from the mekong region, through the upstream region of the yangtze river (uryz) to the middle and upstream regions of the yellow river, the other is from the middle and downstream regions of the yangtze river to the downstream region of the yellow river, and then to northeast china. the uryz was regarded as a passageway of the former dispersal route; however, this assumption remains to be further investig ...201223272130
molecular detection of porcine astrovirus in sichuan province, china.porcine astrovirus (poastv) is widely distributed worldwide, and is highly prevalent among piglets with or without diarrhea, existing as at least five distinct lineages (poastv1-poastv5) within the genus mamastrovirus. however, our knowledge of the diversity and epidemiology of poastv in china is limited.201626739067
streptococcus suis infections in humans: the chinese experience and the situation in north america.infections caused by streptococcus suis are considered a global problem in the swine industry. in this animal species, s. suis is associated with septicemia, meningitis, endocarditis, arthritis and, occasionally, other infections. moreover, it is an agent of zoonosis that afflicts people in close contact with infected pigs or pork-derived products. although sporadic cases of s. suis infection in humans have been reported, a large outbreak due to s. suis serotype 2 emerged in the summer of 2005 i ...200717692141
a potentially novel reovirus isolated from swine in northeastern china in 2007.we report a novel reovirus (mrv-hlj/2007) isolated from swine in heilongjiang province, china. genome sequence analysis indicated a close genetic relationship between mrv-hlj/2007 and strain sc-a, which was isolated from swine in 2006 in sichuan, china. although phylogenetic analysis indicated that mrv-hlj/2007 may have originated from the sc-a strain, the m2 and s3 genes differ between these strains. phylogenetic analysis also showed that, except for differences in the s1 gene, mrv-hlj/2007 and ...201121761235
the complete sequence of the mitochondrial genome of liangshan pig (sus scrofa).liangshan pig is one of the famous native breeds in sichuan province in china. it lives in the mountainous areas (about 1500-2500 m altitude) and has a superior meat quality. here, the complete mitochondrial genome sequence of liangshan pig was first reported. the total length of mitochondrial genome is 16,760 bp, including 34.62% a, 25.79% t, 26.20%, c and 13.39% g, with an a + t bias of 60.41%. mitochondrial genome contains a major non-coding control region (d-loop region), two ribosomal rna g ...201625600741
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