| improved duplex rt-pcr assay for differential diagnosis of mixed infection of duck hepatitis a virus type 1 and type 3 in ducklings. | infection with duck hepatitis a virus (dhav) causes an acute, rapidly spreading, and fatal disease of young ducklings. dhav type 1 (dhav-1) and type 3 (dhav-3) have been identified in china. in this study, a duplex rt-pcr assay was developed to identify dhav-1 and dhav-3 with mixed infection. the method was shown to be high specificity and sensitivity. the minimum detection limit of the method has been determined to be 10pg total rna templates extracted from duck liver samples or 10² copies vira ... | 2013 | 23624117 |
| genetic variation of the vp1 gene of the virulent duck hepatitis a virus type 1 (dhav-1) isolates in shandong province of china. | to investigate the relationship of the variation of virulence and the external capsid proteins of the pandemic duck hepatitis a virus type 1 (dhav-1) isolates, the virulence, cross neutralization assays and the complete sequence of the virion protein 1 (vp1) gene of nine virulent dhav-1 strains, which were isolated from infected ducklings with clinical symptoms in shandong province of china in 2007-2008, were tested. the fifth generation duck embryo allantoic liquids of the 9 isolates were teste ... | 2012 | 22899433 |
| genetic characterization of duck hepatitis a viruses isolated in china. | in recent years, the spread of duck hepatitis a viruses (dhavs) has represented a serious threat and significant economic impact in duck industry of china. the sixteen reported dhav isolates (15 dhav-1 strains and one dhav-3) were identified from infected ducks with clinical symptoms in china between 2009 and 2012. in the present study, the virulence of these viruses and complete sequences of the virion protein 1 (vp1) genes of the 16 dhavs were characterized. the median embryonic lethal doses ( ... | 2013 | 24144860 |
| [sequence analysis of vp1 gene of the duck hepatitis a virus type 3 strains isolated from shandong province of china in 2012]. | to reveal the genetic variation of the viral protein 1 (vp1) gene of the duck hepatitis a virus type 3 (dhav-3), the vp1 gene of 13 virulent dhav-3 strains isolated from shandong province of china in 2012 were amplified by rt-pcr, sequenced and analyzed. the results showed that all the vp1 genes of the 13 isolates contained 720 nucleotides encoding 240 amino acids, and shared with nucleotide identities of 94. 6%-99.9% and amino acid identities of 95.0%-100%. the nucleotide and amino acid sequenc ... | 2013 | 24386841 |
| circulation and in vivo distribution of duck hepatitis a virus types 1 and 3 in infected ducklings. | the circulation of duck hepatitis a virus types 1 (dhav-1) and 3 (dhav-3) in southeast asia has resulted in a continuously changing epidemiological scenario. in this study, a duplex real-time pcr assay for simultaneous quantitative detection of dhav-1 and dhav-3 was established, and 200 liver samples from dead ducklings collected from 31 different flocks in shandong province, china, were tested. fifty-eight (29.0 %) samples from 13 flocks were positive for dhav-1 single infection, 113 (56.5 %) s ... | 2016 | 26597185 |