Publications

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occurrence of theileria and babesia species in water buffalo (bubalus babalis, linnaeus, 1758) in the hubei province, south china.the presence and prevalence of tick-borne haemoparasites in water buffalo from the hubei province, south china was investigated using the reverse line blot (rlb) hybridization assay and phylogenetic analysis of the parasite 18s rrna gene. theileria buffeli (19.1%) was the most frequently found species in all of the locations, followed by babesia orientalis (8.9%), babesia bovis (1.0%) and babesia bigemina (0.7%). only 12 (3.9%) of the samples had mixed infections. eleven samples with single infe ...201122154255
an indirect elisa for detection of theileria sergenti antibodies in water buffalo using a recombinant major piroplasm surface protein.in this study we investigated the prevalence and enzootic potential of theileria spp. in water buffalo in the hubei province in china. an indirect elisa based on a recombinant major piroplasma surface protein was developed. the complete orf of the 33-kda major piroplasma surface protein (p33) was obtained from theileria sergenti genomic dna by pcr, cloned into the pet-28(a) vector and expressed in e. coli as a his-fusion protein. then the recombinant p33 (rp33) was purified and used as the antig ...201020207484
loop-mediated isothermal amplification (lamp) detection of babesia orientalis in water buffalo (bubalus babalis, linnaeus, 1758) in china.loop-mediated isothermal amplification (lamp) is a rapid method with high specificity and efficiency under isothermal condition using a set of four specifically designed primers that recognize six distinct sequences on the target gene. in this study, a lamp method was developed for specific detection of babesia orientalis in water buffalo (bubalus babalis, linnaeus, 1758). four primers were designed from the v4 hypervariable region of the 18s rrna gene of b. orientalis. blood samples were collec ...200919665847
semi-nested pcr detection of babesia orientalis in its natural hosts rhipicephalus haemaphysaloides and buffalo.babesiosis has recently been recognized as an emerging infectious disease of buffalo in china. in order to investigate the epidemiology and enzootic potential of this parasite in hubei province, we sought to develop a semi-nested pcr to detect babesia orientalis in buffalo and the potential tick vector-rhipicephalus haemaphysaloides by amplifying a specific 257bp fragment of b. orientalis 18s rrna gene. the practical limit of detection showed that it had high sensitivity and an approximate paras ...200717005323
serological investigations on babesia orientalis infection. status of water buffaloes in hubei province.a total of 230 water buffaloes were sampled for the determination of the epidemiological situation of babesia orientalis infection in hubei province, using the latex agglutination test. this serological test revealed that 74.7% of the collected sera were positive for antibodies against b. orientalis in endemic areas. in contrast, only 1.79% of water buffaloes were positive in non-epidemic areas. the incidence of the infection was substantially higher in older than in younger animals. the results ...200212051598
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