development of a real-time pcr probe for quantification of the heterotrophic dinoflagellate cryptoperidiniopsis brodyi (dinophyceae) in environmental samples. | a taqman format real-time pcr probe was developed against the internal transcribed spacer 2 ribosomal dna region for the specific detection and quantification of cryptoperidiniopsis brodyi in environmental samples. the assay specificity was confirmed by testing against related dinoflagellates and verified by sequencing pcr amplicons from natural water samples. phylogenetic analysis of the sequenced environmental samples also showed that this assay is specific to c. brodyi. the c. brodyi-specific ... | 2007 | 17322326 |