| integrated molecular diagnosis of theileria and babesia species of cattle in italy. | a reverse line blot hybridization (rlb) test was developed to specifically identify six theileria spp. (t. annulata, t. parva, t. mutans, t. velifera, t. taurotragi, and t. buffeli/orientalis) and three babesia spp. (b. bovis, b. bigemina, and b. divergens). no cross reaction was observed with other livestock pathogens (such as anaplasma marginale, a. centrale, a. ovis, cowdria ruminantium, trypanosoma brucei, t. congolense, and t. vivax). this method was used to test bovine blood samples collec ... | 2000 | 11193668 |
| detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in sicily. | a reverse line blot hybridisation (rlb) of 21 oligonucleotides with polymerase chain reaction (pcr) amplified regions of 16s rrna (ehrlichia/anaplasma group) or 18s rrna (babesia/theileria group) genes of haemoparasites detected theileria annulata, t. buffeli/orientalis, babesia bovis, b. bigemina, b. divergens, ehrlichia bovis, anaplasma marginale, a. centrale and unknown species within the rickettsia tribe.a very high prevalence of mixed infections was detected, which indicated that animals in ... | 2001 | 11511414 |
| genetic diversity of anaplasma marginale strains from cattle farms in the province of palermo, sicily. | bovine anaplasmosis, caused by the tick-borne rickettsia anaplasma marginale, is endemic in sicily and results in economic loss to the cattle industry. this study was designed to characterize strains of a. marginale at the molecular level from cattle in the province of palermo, sicily. seropositivity of cattle >or=1 year old for a. marginale in the study area ranged from 62% to 100%. the observed prevalence of a. marginale infections in cattle herds ranged from 25% to 100%. two predominant a. ma ... | 2005 | 16115096 |
| anaplasmosis in cattle in italy. | bovine anaplasmosis caused by anaplasma marginale is a disease transmitted by ticks belonging to the ixodidae family. southern italy is considered an endemic zone but environmental and social factors are changing the epidemiology of the disease to expand to previously anaplasmosis-free regions. the available data of published reports of anaplasmosis in italy together with the data obtained by the national centre of reference for anaplasma, babesia, rickettsia and theileria (c.r.a.ba.r.t.), allow ... | 2007 | 17682850 |
| prevalence and genotypes of anaplasma species and habitat suitability for ticks in a mediterranean ecosystem. | anaplasma species are tick-transmitted pathogens that impact veterinary and human health. sicily is one of the locations where these pathogens are endemic. sicily represents a typical mediterranean ecosystem to study anaplasma infection and tick habitat suitability. the aims of this study were (i) to characterize by 16s rrna and species-specific msp4 gene pcr the prevalence and genotypes of a. marginale, a. phagocytophilum, and a. ovis in the most abundant host species in sicilian provinces and ... | 2008 | 18978093 |
| a molecular survey of anaplasma spp., rickettsia spp., ehrlichia canis and babesia microti in foxes and fleas from sicily. | fleas (insecta: siphonaptera) are obligate bloodsucking insects, which parasitize birds and mammals, and are distributed throughout the world. several species have been implicated in pathogen transmission. this study aimed to monitor red foxes and the fleas isolated from them in the palermo and ragusa provinces of sicily, italy, as these organisms are potential reservoirs and vectors of pathogens. thirteen foxes (vulpes vulpes) and 110 fleas were analysed by polymerase chain reaction (pcr) to de ... | 2013 | 24589112 |
| characterization of anaplasma infections in sicily, italy. | this study aimed to characterize infection with anaplasma marginale, a. phagocytophilum, a. ovis, and a. platys in humans, animals, and ticks in sicily, italy, during 2003-2006. serologic (competitive elisa [celisa]) and indirect immunofluorescence antibody [ifa]; n= 1990) and dna (polymerase chain reaction [pcr]; n= 2788) tests were conducted on horse, donkey, cattle, sheep, goat, pig, dog, cat, roe deer, wild boar, human, and tick samples. the results reported herein suggested that in sicily c ... | 2008 | 19120181 |
| serologic and molecular characterization of anaplasma species infection in farm animals and ticks from sicily. | although anaplasma marginale was known to be endemic in italy, the diversity of anaplasma spp. from this area have not been characterized. in this study, the prevalence of anaplasma spp. antibodies in randomly selected farm animals collected on the island of sicily was determined by use of a msp5 celisa for anaplasma spp. and an immunofluorescence test specific for anaplasma phagocytophilum. genetic variation among strains of anaplasma spp. from animals and ticks was characterized using the a. m ... | 2005 | 16043300 |