| [studies on the strain differences of schistosoma japonicum in the mainland of china. xi. enzyme linked immunoelectrotransfer blot analysis]. | antigens prepared from s. japonicum adult worms of different isolates i. e. anhui, hubei, guangxi, yunnan and sichuan by origin were analyzed by enzyme linked immunoelectrotransfer blot (eitb) probed with rabbit anti-snail antibody (figs 1,2). anti-sera against oncomelania h. hupensis from anhui, hubei, yunnan and sichuan localities were prepared separately and used in the tests. the eitb patterns were similar in s. j. isolates of anhui and hubei, and it was also the case among s. j. isolates of ... | 1992 | 1303328 |
| [genetic polymorphism of alpha 2hs-glycoprotein in the han population in chengdu]. | the distribution of alpha 2hs-glycoprotein (ahsg) phenotype frequencies in the han population in chengdu was studied using polyacrylamide gel isoelectric focusing followed by immunofixation with rabbit anti-human ahsg serum. two hundred eighty-six serum samples collected at random from unrelated individuals were phenotyped for ahsg. the distribution of ahsg phenotype frequencies was found to be ahsg 1 = 47.20%, ahsg 2 = 8.04% and ahsg 2 - 1 = 44.76%. the observed numbers agreed well with the exp ... | 1991 | 1814809 |
| characteristics of different isolates of schistosoma japonicum from china in the final hosts. | the present paper deals with the susceptibility of common laboratory animals, such as mouse, rat, hamster, jird, rabbit and rhesus monkey, to infection with different isolates of schistosoma japonicum in the mainland of china under laboratory conditions. with the exception of the rat, all the animals under study were permissive hosts for different isolates though their worm recovery rates varied. the mean body length of pair-worms of the yunnan isolate was considerably smaller than that of the a ... | 1991 | 1948285 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. iv. fuzzy cluster analysis on some biological characters of different isolates of schistosoma japonicum]. | in this paper, a computerized numerical systematics was used to analyse the 5 different isolates of schistosoma japonicum in the mainland of china by means of fuzzy clustering method with 32 quantitative data of each isolate about the morphological and biological characters. a dendrogram of the 5 isolates of s. japonicum was constructed on the basis of similarity coefficients as shown. the phylogenetic relationships revealed by the present study show that the two isolates of anhui and hubei get ... | 1991 | 1959166 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. iii. morphometric data]. | the present investigation was undertaken to determine whether differences exist in the size of cercariae, adult worms and eggs among different isolates of s. japonicum in the mainland of china. measurements on the corresponding stages of japanese and philippine strains of s. japonicum were also carried out for comparison. the size of cercariae collected from pooled naturally infected snails from different isolates was shown in table 1. the body index of yunnan isolate, which is defined as cercar ... | 1991 | 2065446 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. ii. susceptibility of mammalian hosts]. | six species of animals were percutaneously exposed to cercariae obtained from pools of naturally infected snails from different isolates of s. japonicum in the mainland of china as shown in tables 1 and 2. our results indicated that with the exception of rat, all animals under study were permissive host though their worm recovery rates varied with different isolates. the mean prepatent period in different host species were 35.0 +/- 0.8 to 36.4 +/- 1.0 days for anhui isolate; 34.5 +/- 1.2 to 36.4 ... | 1990 | 2099256 |
| [a serological survey of legionnaires' disease in domestic fowls and animals in chengdu area]. | a serological survey of antibodies against legionella pneumophila (lp) serotypes in chengdu area by microagglutination test showed that there were high levels of antibodies against serotypes lp1 and lp6 in healthy domestic fowls and animals (rabbits, pigs, chickens, ducks and geese). in rabbits, antibodies against serotypes lp1-lp6 were determined, showing positive rates (titer greater than or equal to 1:16) ranging 6.3-23.8%. among them only the serotype lp3 did not show any positive one. the h ... | 1989 | 2630424 |
| sds-page protein pattern and its antigenicity analysis of different isolates of schistosoma japonicum in china. | homogenates prepared from s. japonicum adult worms of different isolates from anhui, hubei, guangxi, yunnan and sichuan provinces were analyzed by sds-page and enzyme linked immunoelectrotransfer blot (eitb) tested with rabbit anti-snails antibody. the results of sds-page indicated that with silver staining both male and female worms of guangxi isolate showed some definite differences in their protein profile, namely, absence of one band between 50-75 kda in male worms and marked reduction in qu ... | 1994 | 8187569 |
| variation in the sequence of a mitochondrial nadh dehydrogenase i gene fragment among six natural populations of schistosoma japonicum from china. | the present study investigated the level of genetic variation among schistosoma japonicum populations of different geographical origins from mainland china. polymerase chain reaction-based methods were employed to determine the sequence for a subunit of the mitochondrial nadh dehydrogenase i gene for populations from zhejiang, anhui, jiangxi, hunan, hubei and sichuan. no variation was detectable in the nadh dehydrogenase i sequence within populations from zhejiang and hubei, whereas sequence var ... | 1998 | 9925274 |
| an electrophoretic comparison of schistosoma japonicum (trematoda) from different provinces in the people's republic of china suggests the existence of cryptic species. | schistosoma japonicum from the people's republic of china is considered to represent a single species comprising either 1 or 4 'strains'. we conducted an allozyme electrophoretic study to examine the extent of genetic variation in s. japonicum from mainland china. the allelic profiles of s. japonicum from 7 provinces were established at 16 enzyme loci. s. japonicum from sichuan had 3-5 (19-31%) fixed differences compared with those from zhejiang, anhui, jiangxi, hunan, hubei and yunnan, suggesti ... | 1999 | 10581616 |
| [male contraceptive -- the biological compatibility evaluation of hydrogel hfmc]. | it was found from experiments in animals that hfmc hydrogel compound injected into the vas-deferens becomes a hydrogen molecule-releasing sediment, which changes the ph balance inside the vas deferens and can kill the sperm and achieve contraception. hfmc hydrogel can either dissolve in time or through an injection of dmso solution, and fertility will resume subsequently. for the purpose of further developing hfmc hydrogel into a safe and effective contraceptive method, biological compatibilit ... | 1992 | 12285893 |
| [polymorphisms and species specificity of d2s2944 and d1s2134 loci in chinese han population in chengdu]. | to obtain the data of polymorphism distribution of the two str loci d2s2944, d1s2134 in chinese han population in chengdu and evaluate their usefulness in the field of forensic science. | 2004 | 15573761 |
| [isolation and identification of streptococcus suis serotype 2 from sick-pig samples of sichuan province]. | streptococcus suis serotype 2 (ss2) is a major pathogen frequently associated with infections in pigs. there are presently 35 serotypes of s.suis (serotype 1 to 34 and serotype 1/2) recognized on the basis of capsular antigens. few people were reported to infect with ss2 in the past years. however, an accidental case happened in sichuan province of china in 2005. some people got ill and died, and all of them were closely contacted with sick pigs. based on clinical features and epidemiologic data ... | 2006 | 17037069 |
| [allele frequencies and species specificity of five short tandem repeat loci of chinese han population in chengdu]. | to obtain the data in polymorphism distribution of the five short tandem repeat (str) loci: d18s979, d11s2014, d18s548, d1s1667 and gata164f07 of chinese han population in chengdu, and to evaluate their usefulness in the field of species specificity in forensic science. | 2006 | 17160956 |
| [study on the cloning, in vitro expression and bioactivity of ailuropoda melanoleuca qinling subspecies interleukin-2]. | to identify if any difference exisit between the population of sichuan and qinling giant panda. express il-2 protein in bl21(de3)with biological activity. | 2009 | 19500497 |
| issr, an effective molecular approach for studying genetic variability among schistosoma japonicum isolates from different provinces in mainland china. | in the present study, inter-simple sequence repeats (issrs) markers were used to examine the genetic variability of schistosoma japonicum isolates from different provinces in mainland china, using s. japonicum from japan and s. mansoni from puerto rico for comparison. of the 30 primers screened, 4 produced highly reproducible issr fragments. using these primers, 107 discernible dna fragments were generated with 105 (98.13%) being polymorphic, indicating considerable genetic variation among the e ... | 2009 | 19539784 |
| streptococcus agalactiae infection in domestic rabbits, oryctolagus cuniculus. | streptococcus agalactiae (group b streptococcus, gbs) has emerged as an important pathogen that affects humans and animals, including aquatic species. in august 2011, a severe infectious disease affecting rabbits, which caused 42% mortality, occurred in mianyang, sichuan province, china. the main clinical signs included acute respiratory distress syndrome, fever, paddling and convulsions. a gram-positive, chain-forming coccus was isolated from the primary organs and tissues of diseased rabbits a ... | 2014 | 23465082 |