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[epidemiological survey on the infection of hepatitis e virus among pigs in henan province].to investigate hepatitis e virus (hev) infection among pigs in henan province.200818414692
phylogenetic analysis of mycoplasma suis isolates based on 16s rrna gene sequence in china.to perform phylogenetic analysis of mycoplasma suis isolates derived from china to define the nature of this pathogen, nearly complete of 16s rrna genes from chongqing, sichuan, henan and guangdong isolates were amplified by pcr and sequenced. the four sequences from the blood samples in this study, with other 17 hemoplasmas sequences and related 3 mycoplasma sequences available in the genbank, were aligned using clustal x (version 1.83) sequences alignment program. maximum parsimony, neighbor-j ...200919590972
cloning, in vitro expression, and bioactivity of interleukin-18 isolated from a domestic porcine breed found in henan.to evaluate the effects of recombinant porcine interleukin-18 (rpil-18) on the replication of viruses in host cells and proliferation of lymphocytes, porcine il-18 (pil-18) isolated from a domestic big-white porcine breed found in the henan province (hn) was cloned using a reverse transcriptase-pcr. the cloned hn pil-18 contained an orf of 579 base pairs encoding a 192-amino-acid precursor protein. the amino acid sequence of hn pil-18 was compared with all the other pil-18 amino acid sequences a ...200919732142
[surveillance and control of japanese encephalitis in henan province, 1950 - 2008].to analyze the characteristics and trend of japanese encephalitis (je) in henan province.200920193229
further evidence for infection of pigs with human-like h1n1 influenza viruses in china.classical swine and avian-like h1n1 influenza viruses were reported widely in swine population worldwide, but human-like h1n1 swine viruses were reported occasionally. in 2006, a human-like h1n1 swine virus (a/swine/guangdong/96/06) was isolated from pigs in guangdong province, which was reported in china for the first time. to get further evidence for infection of pigs with human-like h1n1 influenza viruses, we analyzed eight gene segments of three human-like swine h1n1 viruses (a/swine/guangdo ...200919063926
swine infection with h9n2 influenza viruses in china in 2004.in 2004, h9n2 influenza a viruses were isolated from pigs with respiratory syndrome in commercial swine farms in henan province, china. antigenic and genetic characterization were performed for seven swine h9n2 influenza viruses. the hemagglutinin antigenicity of swine h9n2 viruses was similar to those of avian h9n2 viruses of a/duck/hong kong/y280/1997 (dk/hk/y280/97)-like sublineage prevalent in china. it is noteworthy that the neuraminidase of these isolates had no deletions in the stalk, whi ...200818401696
genome comparison of a novel classical swine fever virus isolated in china in 2004 with other csfv strains.the genome of a novel classical swine fever virus (csfv), swh/ca/2004, isolated from a hog pen in henan province, central china, is 12,296 nucleotides (nt) in length. it is composed of a 373-nt 5' terminal non-translated region (ntr), a 11,697-nt open reading frame (orf) encoding a polyprotein of 3,898 amino acids (aa), and a 226-nt 3'-ntr. genome comparison of the swh/ca/2004 isolate (genbank accession: dq127910) with other known csfv isolates was performed and analyzed. corresponding segments ...200616972026
outbreaks of human trichinellosis caused by consumption of dog meat in china.dog meat has become an important source of trichinella infection for humans in china. the first documented outbreak of human trichinellosis resulting from the consumption of dog meat occurred in china in 1974. until 1999, the outbreaks with this source of infection occurred mostly in northeast of china (81 cases in five outbreaks in jilin and two in liaoning), beijing (six cases) and henan provinces (two cases). the epidemiological surveys were performed in nine provinces or autonomous regions o ...200111484389
epidemiological survey of trichinella infection in some areas of henan province.during 1996-1998 epidemiological survey of domestic trichinella spiralis infection and serological survey on human beings were carried out in yanling, xuchang counties and luohe city. by the artificial digestion method, the positive rates of pork in market and mutton were 1.6% and 0.6% respectively. meat samples of pigs from pig-farms, cats and dogs were all negative for trichinella larvae. trichinellosis infection levels in rats captured in peasant households and places around abattoirs were 0. ...200111484388
epidemiology of swine trichinellosis in china.swine trichinellosis has been reported in 26 provinces, autonomous regions or municipalities (p/a/m) of china. the prevalence rate in swine varied from 0.12% to 34.2% in five p/a/m, from 0.01% to 0.0001% in other p/a/m. the seroepidemiological survey of swine trichinellosis was carried out by elisa in seven p/a/m, the seroprevalence varied from 0.09% to 29.63%. the prevalence of trichinella infection in pork sold at the market was from 0.29% to 5.6% in four provinces. the transmission of trichin ...200111484386
epidemiological, clinical and serological studies on trichinellosis in henan province, china.the epidemiological, clinical and serological studies on human trichinellosis in henan province were carried out from january 1992 to december 1996. the results showed that 467 patients attending our department came from 12 administrative areas and cities of the province and they acquired the infection mainly by eating undercooked dumplings, tasting the raw pork filling for dumplings, ingesting scalded pork or mutton. there was the high incidence season of trichinellosis during the winter. most ...19989879735
analysis of chinese isolates of trichinella spiralis by molecular biotechnology.to investigate the relationships among trichinella spiralis isolates obtained from different hosts and geographical regions in china.19969275332
seven outbreaks of trichinosis in china (1992-1996).seven outbreaks of trichinosis occurred in zhengzhou (central china) from 1992 to 1996, 250 patients were diagnosed. trichinella larvae were found in 3 of 4 portions of the pork examined and in 5 of 7 cases by muscle biopsy. there was a higher occurrence of outbreaks in winter. young and middle-aged workers, cadres and merchants were the majority of patients, (higher in males than in females). the most important clinical manifestations were fever, eyelid edema, tiredness and eosinophilia without ...19979257992
restriction fragment length polymorphism (rflp) analysis of genomic dna of 5 strains of trichinella spiralis in china.five restriction endonucleases were used to digest genomic dna from 5 isolates of trichinella spiralis obtained from changchun, tianjin, xian, henan and yunnan. all the isolates were secured from pigs except the changchun strain which came from dog. the dna fragments digested by endonuclease were separated by agarose gel electrophoresis. the changchun isolate had a ecori band at 1.12kb and a drai band at 1.97kb which were unique to this isolate. a cloned specific repetitive dna sequence (1.12kb) ...19958580480
swine and rabbits are the main reservoirs of hepatitis e virus in china: detection of hev rna in feces of farmed and wild animals.hepatitis e virus (hev) infection is recognized as a zoonosis. the prevalence of hev rna and anti-hev antibodies in many animal species has been reported, but the host range of hev is unclear. the aims of this study were to investigate hev infection in various animal species and to determine the reservoirs of hev. eight hundred twenty-two fecal samples from 17 mammal species and 67 fecal samples from 24 avian species were collected in china and tested for hev rna by rt-npcr. the products of pcr ...201526303139
social complexification and pig (sus scrofa) husbandry in ancient china: a combined geometric morphometric and isotopic approach.pigs have played a major role in the economic, social and symbolic systems of china since the early neolithic more than 8,000 years ago. however, the interaction between the history of pig domestication and transformations in chinese society since then, have not been fully explored. in this paper, we investigated the co-evolution from the earliest farming communities through to the new political and economic models of state-like societies, up to the chinese empire, using 5,000 years of archaeolo ...201627384523
Genetic and virulence characterization of Streptococcus suis type 2 isolates from swine in the provinces of Zhejiang and Henan, China.This study was aimed to examine the genetic characteristics of 44 Streptococcus suis type 2 (SS2) isolates and the virulence attributes of 23 representative isolates. Multilocus sequence typing revealed five sequence types (ST1, ST7, ST28, ST86, and ST162) with 19 isolates assigned to ST7 (43.2%), 14 to ST1 (31.8%), and 9 to ST28 (20.5%). PCR typing of the pilus gene clusters classified the isolates into three types: A (72.7%), B (22.7%), and N (4.5%). All isolates of pilus types A and N were as ...201122083785
identification and characterization of micrornas in trichinella spiralis by comparison with brugia malayi and caenorhabditis elegans.trichinella spiralis is an important zoonotic nematode causing trichinellosis which is associated with human diseases such as malaise, anorexia, nausea, vomiting, abdominal pain, fever, diarrhea, and constipation. micrornas (mirnas) are endogenous small non-coding rnas that play important roles in the regulation of gene expression. the objective of the present study was to examine the mirna expression profile of the larvae of t. spiralis by solexa deep sequencing combined with stem-loop real-tim ...201121327987
an epidemiological overview of swine trichinellosis in china.trichinellosis is a major food-borne zoonosis with health, social, and economic impacts. epidemiological data on swine trichinellosis in china from 2005 to 2009 were obtained from seven provinces/autonomous regions/municipalities (p/a/m) and analyzed and sero-epidemiological data were acquired from five p/a. the seroprevalence ranged from 0.01% to 29.95% as determined by enzyme-linked immunosorbent assay or an immunochromatographic strip method. the prevalence of trichinella infection in swine s ...201121296013
[sequencing of mitochondrial dna cytochrome oxidase subunit i gene in sarcosaphagous flies from 14 provinces in china].to detect the 278 bp region of gene of the cytochrome oxidase subunit i (coi) in mitochondral dna (mtdna) of sarcosaphagous flies, identify the species of sarcosaphagous flies, and provide reference for forensic application.201020818074
prevalence and molecular identification of cryptosporidium spp. in pigs in henan, china.the distribution and public health significance of cryptosporidium species/genotypes in pigs differ among geographic areas and studies. to characterize the prevalence of cryptosporidiosis in pigs in henan, china, a total of 1,350 fecal samples from 14 farms in ten prefectures in henan province were examined. the overall prevalence of cryptosporidium was 8.2% (111/1,350), with the highest infection rate (79/383 or 20.6%) in 1-2-month-old piglets and the lowest infection rates in 3-6-month-old pig ...201020697908
[the prevalence characteristic and prevention strategy of japanese b encephalitis in henan province].to analyze the prevalence trend, prevalence characteristics and influence factors of japanese b encephalitis (je) in henan province.201020654146
genetic characterization of toxoplasma gondii isolates from pigs in china.toxoplasma gondii genetic diversity varies in different geographical regions. in south america, t. gondii isolates are highly diverse, whereas in north america and europe, the parasite is highly clonal, with 3 distinct lineage types (i, ii, iii). however, little is known of the t. gondii genotypes in the people's republic of china. because pork is considered the principal meat source for t. gondii infection in china, we conducted a survey to determine the prevalence and genetic diversity of this ...201020481661
stx2vha is the dominant genotype of shiga toxin-producing escherichia coli o157:h7 isolated from patients and domestic animals in three regions of china.shiga toxin-producing escherichia coli(stec) o157: h7 strains were isolated from domestic animals and patients from xuzhou city, jiangsu province, china and the bordering anhui and henan provinces and were examined for the stx genotype. of 390 strains, 277 were identified as genotype stx2vha ; 41, stx2 ; 51, stx2-stx1 ; 1, stx2-stx2vha-stx1 ; 5, stx2-stx2vha ; and 15 were un-typeable. of the 277 stx2vha-bearing isolates, 116 were isolated from goats; 42, cattle; 38, hens, and 35 from pigs. the s ...200516365526
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