| production of toxins by escherichia coli strains isolated from calves with diarrhoea in galicia (north-western spain). | a total of 289 escherichia coli colonies isolated from 78 diarrhoeic calves were studied for production of heat-labile (lt) and heat-stable (sta) enterotoxins, verotoxin (vt), cytotoxic necrotizing factor (cnf) and k99 antigen, and they were serotyped. production of sta was detected in a single strain possessing both k99 and f41 antigens; the serotype was 09:k (a) 35. lt-producing strains were not detected. from 16 (20.5%) calves, 51 vt-producing colonies of e. coli were isolated. production of ... | 1988 | 3068896 |
| enterotoxigenic and enteropathogenic escherichia coli in galicia (north-west spain). | we have studied the incidence of enterotoxigenic and enteropathogenic escherichia coli strains associated with infant diarrhoeal disease in galicia (north-west spain). during a period of 9 months we isolated heat-labile enterotoxin-positive strains in 2.1% of children with diarrhoea examined, whereas the production of heat-stable enterotoxin was detected in 1.1% of them. enteropathogenic strains were isolated from 5.3% of the children with diarrhoea, but none of these strains released heat-labil ... | 1983 | 6358820 |
| enterotoxigenic, verotoxigenic, and necrotoxigenic escherichia coli isolated from cattle in spain. | to assess the role of enterotoxigenic (etec), verotoxigenic (vtec), and necrotoxigenic (ntec) escherichia coli in cattle with diarrhea, 1,524 colonies of e coli isolated from 197 calves with diarrhea and from 112 healthy controls were investigated for production of heat-labile and heat-stable enterotoxins, verotoxins, and cytotoxic necrotizing factors (cnf1 and cnf2). the etec were isolated from only 2 (1%) calves with diarrhea and from 5 (4%) healthy controls. in contrast, vtec and ntec that pr ... | 1993 | 8239131 |
| prevalence and characteristics of enteropathogenic escherichia coli with the eae gene in diarrhoeic rabbits. | a field study was carried out with the objective of investigating the prevalence of enteropathogenic escherichia coli (epec) with the eae gene in diarrhoeic rabbits. epec eae+ were isolated from 60 (74%) of 81 diarrhoeic rabbits sampled in 30 industrial fattening farms localized in the four provinces of galicia (northwestern spain). attaching and effacing lesions were found in 44 of 50 animals processed for histology. the 111 e. coli strains identified belonged to 19 different o serogroups and 1 ... | 1997 | 9087949 |
| use of a sandwich-enzyme-linked immunosorbent assay (sea) for the diagnosis of natural fasciola hepatica infection in cattle from galicia (nw spain). | an indirect-enzyme immune-linked immunosorbent assay (iea) was compared to a sandwich-enzyme-linked immunosorbent assay (sea) to diagnose fasciolosis in naturally infected cattle. by means of a rabbit polyclonal igg antibody to f. hepatica antigens, captured circulating antigens were detected by an sea technique. a total of 85.1% of the samples examined was considered positive by using the iea-test, whereas this percentage was of 37.3% by the sea test. using the two enzymatic probes, only the 38 ... | 2000 | 11027859 |
| enterocytozoon bieneusi (microsporidia) in faecal samples from domestic animals from galicia, spain. | in this survey we examined 87 domestic animal stool samples in order to detect the possible presence of microsporidia in animals in close contact with humans in galicia (nw, spain). the detection of enterocytozoon bieneusi spores was confirmed in faecal samples from two dogs and one goat by polymerase chain reaction. none of the positive samples for microsporidia in the staining method were amplified with species-specific primers for encephalitozoon intestinalis, e. hellem and e. cuniculi. four ... | 2002 | 12471418 |
| prevalence of natural ovine fasciolosis shown by demonstrating the presence of serum circulating antigens. | the prevalence of fasciolosis in sheep (galicia, northwest spain) kept under field conditions was determined by using a sandwich-enzyme-linked immunosorbent assay (selisa). serum fasciola hepatica circulating antigens were captured by means of a rabbit polyclonal igg antibody to f. hepatica excretory/secretory products. results were compared to those obtained by faecal sedimentation and an indirect elisa (ielisa) and excretory/secretory antigens. prevalences were 39.1% by selisa, 30.4% by faecal ... | 2003 | 14574565 |