[genetic analysis of west nile fever virus, isolated in the south of the russian plain (volgograd and astrakhan regions) in 1999].two strains of west nile virus, vlg 27889 and ast 986, were isolated from the brain of a dead man and from the blood of a patient, respectively, during an outbreak of serous meningitis and meningoencephalitis in july-september, 1999, in the volgograd and astrakhan regions. analysis of parts of genome of the strains cloned from cell culture by reverse transcription and polymerase chain reaction demonstrated their identity and appurtenance to group i west nile viruses.200111233290
[the isolation of dhori viruses (orthomyxoviridae, thogotovirus) and crimean-congo hemorrhagic fever virus (bunyaviridae, nairovirus) from the hare (lepus europaeus) and its ticks hyalomma marginatum in the middle zone of the volga delta, astrakhan region, 2001].in august, 2001, in the middle zone of the delta of the volga river, the astrakhan region, during investigation of the natural foci of west nile fever and crimean--congo hemorrhagic fever (cchf), the material from the hare (lepus europaeus, pallas, 1778 (lagomorpha, leporidae) and collected from it the ticks hyalomna marginatum koch 1844, was obtained. 4 strains of dhori virus (orthomyxoviridae, thogotovirus) and 2 strains of cchf virus (bunyaviridae, nairovirus) were isolated. this is the first ...200212271723
[ west nile fever among residents of the astrakhan region in 2002].thirty-three persons infected with west nile fever were detected in 2002 in the astrakhan region; the diagnosis was confirmed serologically and the maximal number of the infected was registered in august, same year. the indices of the specific humoral immunity varied from 3.3% to 27.1%. a monitoring determined the highest infection risk among the residents of the volga middle delta.200314598473
[comparative characteristics of transmissive fevers in the territory of astrakhan region (clinicoepidemiological evidence)].epidemiologists register a trend in the latest decades for expansion of the areas of natural-focal transmissive fevers morbidity in the astrakhan region. this is explained by reconfiguration of natural landscapes as a result of increasing anthropogenic impact. the astrakhan territory exclusively contains 4 types of landscape with evolutionally formed several natural foci of transmissive fevers: crimean hemorrhagic fever, west nile fever, astrakhan rickettsial fever, q-fever. early diagnosis of t ...201122312887
[the need for human health protection from climate changes].the occurring climate changes have a considerable impact on human health. the higher frequency of different natural disasters, including flood and typhoons, deteriorates the epidemiological situation. evidence is provided for the importance of climate warming as a risk factor for a number of communicable diseases, including west nile fever in the astrakhan and volgograd regions. the paper presents the results of analytical epidemiological surveys, by using the time-series analysis, in tver and m ...200920000092
[the influence of weather conditions on the epidemiology of vector-borne diseases by the example of west nile fever in russia].climate changes must influence the incidence of vector-borne infections, but their effects cannot be revealed due to lack of long-term observations. the impact of short-term weather changes may be used as a model. in russia the biggest numbers of clinical cases of mosquito-borne west nile infection were registered in 1999 in volgograd and astrakhan regions. the analysis of climatic dataset since 1900 shows that 1999 was the hottest year in volgograd in the 20th century due to a very mild winter ...200616544901
[isolation of the west nile fever virus from human patients during an epidemic outbreak in the volgograd and astrakhan regions].two strains of west nile virus leiv 27889 vig and ast 986 were isolated from the brain of a dead subject and from the blood of a patient, respectively, during an outbreak of serous meningitis and meningoencephalitis in july-september, 1999, in the volgograd region, krasnodar territory, and astrakhan region. these strains reacted with convalescent sera in hemagglutination inhibition test, which proves their etiological role in this outbreak.200010867987
[west nile virus infection of agricultural animals in the astrakhan region, as evidenced by the 2001-2004 serological surveys].sera sampled from 2,884 farming animals in the astrakhan region in 2001 to 2004 were investigated by the hemagglutination inhibition test (hit) in order to indicate specific antibodies to west nile virus (wnv). hit-positive samples were investigated by the neutralization test (nt). wnv antibodies were detected in all the examined species of animals: horses (the proportion of positive tests throughout the observation averaged 9.8%; the agreement with nt results was 94.1%), cattle (6,4 and 72.%), ...200516408630
[information technologies in epidemiological control of natural-focal infectious diseases].an approach to increase effectiveness of epidemiological control of crimean hemorrhagic fever, west nile fever and astrakhan spotted fever by building a prognostic model of epidemic activity based on contemporary information technologies of spatial and intellectual data analysis was developed and tested. personified data on 4505 laboratory confirmed cases of natural-focal infectious diseases registered in the russian federation and database on 1999 - 2011 climatic observations were processed. a ...201323805655
the incidence of west nile disease in russia in relation to climatic and environmental factors.since 1999, human cases of west nile fever/neuroinvasive disease (wnd) have been reported annually in russia. the highest incidence has been recorded in three provinces of southern european russia (volgograd, astrakhan and rostov provinces), yet in 2010-2012 the distribution of human cases expanded northwards considerably. from year to year, the number of wnd cases varied widely, with major wnd outbreaks in 1999, 2007, 2010, and 2012. the present study was aimed at identifying the most important ...201424464233
[circulation of west nile virus (flaviviridae, flavivirus) and some other arboviruses in the ecosystems of volga delta, volga-akhtuba flood-lands and adjoining arid regions (2000-2002)].comprehensive virological, serological as well as genetic studies of the ecology of west nile virus (wnv) as well as of some other arboviruses were undertaken in different ecosystems in the territories of the astrakhan region and of the kalmyk republic. the main carriers (mosquitoes, ticks, birds and mammals) were defined as involved in the circulation of viruses within the natural and anthropogenic biocenosis. phylogenetic examinations of isolated strains and samples, which were positive in rt- ...200415188655
[screening of birds in the volga delta (astrakhan region, 2001) for the west nile virus by reverse transcription-polymerase chain reaction].infection of birds, residing in the volga lower and middle delta, with west nile virus (wnv) genome was detected by the rt-pcr method. a total of 315 samples of bird organs, collected in the astrakhan region in august 2001, were examined. positive results, with various severity degrees, were found in coots (15.1%) and in cormorants (14.3%) in the lower delta. as for the middle delta, the infection rate among coots, herons, sea-gulls and terms was found to be identical and amounted to 8-13%, it w ...200312608054
[isolation of the west nile fever virus from the great cormorant phalacrocorax carbo, the crow corvus corone, and hyalomma marginatum ticks associated with them in natural and synanthroic biocenosis in the volga delta (astrakhan region, 2001)].four strains identified as west nile fever virus by inhibited hemagglutination and neutralization tests, enzyme immunoassay, and reverse transcription polymerase chain reaction were isolated during a virological examination of birds and their collected ticks in the natural and synanthropic biocenoses of the volga delta. the strains were isolated from the great cormorant (phalacrocorax carbo), the crow (corvus corone) and its collected hyalomma marginatum nymphs. the types of interpopulational re ...200212522961
[the study of immune structure of astrakhan region population to west nile virus in 1998 and 1999].immunostructure of the astrakhan region population to west nile fever (wnf) was studied in the preepidemic period (1998) and after the outbreak (1999). among the sera obtained in 1998, 63 (26.3%) were positive in neutralization reaction, 84 (27.1%) in enzyme immunoassay igg and 20 (7.8%) in hair. igm-antibodies were found in none of 142 samples. overall number of donors having antibodies to wnf virus by three reactions reached 97 (31.6%). in the sera obtained in 1999, virus neutralizing antibodi ...200212508676
[genotyping of west nile fever virus strains circulating in southern russia as an epidemiological investigation method: principles and results].characteristic of west nile fever (wnf) virus strains circulating in southern russia.201121598612
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