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molecular characterization of anisakis pegreffii larvae in pacific cod in japan.it is now recognized that the morphospecies anisakis simplex is not a single species but a complex composed of three sibling species, a. simplex sensu stricto, a. pegreffii and a. simplex c. in japan, a. simplex-like larvae have been isolated from a variety of fish and humans, but the larvae collected have been identified as a. simplex by only light microscopy. therefore, the epidemiology of the a. simplex complex, composed of three sibling species, is still unclear in japan. in the present stud ...200516336712
molecular identification of anisakis simplex sensu stricto and anisakis pegreffii (nematoda: anisakidae) from fish and cetacean in japanese waters.parasites morphologically consistent with anisakis simplex sensu lato collected from the coast of japan and western north pacific ocean were examined by pcr-rflp of the its region (its1, 5.8 subunit rrna gene and its2) and mtdna cox1. the rflp patterns of rdna generated by hinfi and hhai showed that 100% of the larvae collected from hokkaido and 94% of adults collected from western north pacific ocean were identified as a. simplex sensu stricto. on the other hand, 97% of the larvae collected fro ...200616942906
molecular identification of the etiological agent of the human anisakiasis in japan.anisakis simplex complex presently comprises three sibling species, a. simplex sensu stricto, a. pegreffii and a. simplex c. a. simplex is a common parasite in fishes and cephalopods and capable of causing anisakiasis in humans. therefore, identification of sibling species of a. simplex was important for human health. in this study, one hundred anisakis type i larvae isolated from eighty five patients with anisakiasis in hokkaido and kyushu in japan were analyzed by adapting the new molecular me ...200717428725
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