Characterization of epidemic diarrhea outbreaks associated with bovine torovirus in adult cows. | Bovine torovirus (BToV) is recognized as an enteric pathogen of calves, but its etiological role in diarrhea and epidemiological characterization in adult cows remain unclear. In 2007-2008, three outbreaks of epidemic diarrhea occurred in adult cows at three dairy farms in Niigata Prefecture, Japan. BToV was the only enteric pathogen detected in these outbreaks, as determined by electron microscopy, reverse transcription-PCR, bacteria and parasite tests of fecal samples, and antibody tests with ... | 2011 | 22167249 |
genetic and antigenic characterization of newly isolated bovine toroviruses from japanese cattle. | torovirus, a member of the coronaviridae family, is a gastrointestinal infectious agent that has been identified in humans, cattle, pigs, and equines. toroviruses, except equine torovirus, are difficult to propagate in cell culture; indeed, to date, only the aichi/2004 strain of bovine torovirus (btov) has been isolated among the human, bovine, and porcine toroviruses. in the present study, four cytopathogenic btovs were isolated from diarrheal feces of the cattle using the hrt-18 cell line, and ... | 2010 | 20220164 |
first isolation of cytopathogenic bovine torovirus in cell culture from a calf with diarrhea. | a cytopathogenic virus (designated the aichi/2004 strain) was isolated in a human rectal adenocarcinoma cell line (hrt-18) from the ileum contents of a calf with diarrhea. oval and elongated particles, approximately 100 to 170 nm in diameter, with club-shaped projections were seen in the infected culture supernatant, and torovirus-like (tubular and torus nucleocapsid) structures were seen in the infected cells by electron microscopy. an antiserum against bovine torovirus (btov) reacted with the ... | 2007 | 17567770 |