| supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in india. | isolates of two distinct begomovirus species, the severe strain of the species tomato leaf curl new delhi virus (tomato leaf curl new delhi virus-[india:new delhi:severe:1992]; tolcndv-[in:nd:svr:92], bipartite) and the varanasi strain of the species tomato leaf curl gujarat virus (tomato leaf curl gujarat virus-[india:varanasi:2001]; tolcgv-[in:var:01], mono/bipartite) infect tomato (lycopersicon esculentum) and cause severe yield losses in northern india. this study investigated the infectivit ... | 2008 | 18272774 |
| molecular characterization of a distinct bipartite begomovirus species infecting tomato in india. | a distinct bipartite begomovirus was found associated with tomato plants showing yellowing, curling, and crumpling of the leaves, in a sub-temperate region in india. the complete dna-a and dna-b components were amplified through rolling circle amplification (rca) using phi-29 dna polymerase and characterized. the dna-a of the isolate was comprised of 2,756 nucleotides, encoding six open reading frames (orfs) and dna-b that of 2,725 nucleotides, encoding two orfs. genome organization of the isola ... | 2008 | 18792773 |
| molecular characterization of a new species of begomovirus and betasatellite causing leaf curl disease of tomato in india. | a new tomato-infecting begomovirus and cognate betasatellite were characterized from the gangetic plain of northern india. genome organization of this virus was found to be similar to those of other old world begomoviruses. the dna-a molecule (2752nt) shared maximum (85.8%) identity with tomato leaf curl laos virus-[laos] (tolclv-[la]; af195782) from laos and betasatellite molecule (1349nt) shared maximum (75.8%) identity with tomato leaf curl joydebpur betasatellite (tolcjob-[bd:gaz:05]; aj9662 ... | 2010 | 20540978 |
| a novel recombinant tomato-infecting begomovirus capable of transcomplementing heterologous dna-b components. | the genome of a tomato-infecting begomovirus from ranchi, india, was cloned, sequenced and analysed. the viral genome shared 88.3% sequence identity with an isolate belonging to the species tobacco curly shoot virus (tbcsv), and this virus should therefore be considered a member of a new species, tentatively named tomato leaf curl ranchi virus (tolcrnv). the dna-ß molecule, which had 74.5% sequence identity with tomato leaf curl bangladesh betasatellite (tolcbdb), is named tomato leaf curl ranch ... | 2011 | 21311922 |
| molecular characterization and infectivity of a tomato leaf curl new delhi virus variant associated with newly emerging yellow mosaic disease of eggplant in india. | begomoviruses have emerged as serious problem for vegetable and fiber crops in the recent past, frequently in tropical and subtropical region of the world. the association of begomovirus with eggplant yellow mosaic disease is hitherto unknown apart from one report from thailand. a survey in nagpur, central india, in 2009-2010 showed severe incidence of eggplant yellow mosaic disease. here, we have identified and characterized a begomovirus responsible for the newly emerging yellow mosaic disease ... | 2011 | 21676270 |
| association of tomato leaf curl new delhi virus dna-b with bhendi yellow vein mosaic virus in okra showing yellow vein mosaic disease symptoms. | okra samples showing yellow vein mosaic, vein twisting and bushy appearance were collected from different locations of india during the surveys conducted between years 2005-2009. the dot blot and pcr detection revealed that 75.14% of the samples were associated with monopartite begomovirus and remaining samples with bipartite virus. whitefly transmission was established for three samples representing widely separated geographical locations which are negative to betasatellites and associated with ... | 2015 | 26104329 |
| synergistic interaction among begomoviruses leads to the suppression of host defense-related gene expression and breakdown of resistance in chilli. | chilli (capsicum sp.) is one of the economically important spice and vegetable crops grown in india and suffers great losses due to the infection of begomoviruses. conventional breeding approaches have resulted in development of a few cultivars of chilli resistant to begomoviruses. a severe leaf curl disease was observed on one such resistant chilli cultivar (capsicum annuum cv. kalyanpur chanchal) grown in the experimental field of the jawaharlal nehru university, new delhi. four different vira ... | 2016 | 26780359 |
| molecular characterization of a distinct bipartite begomovirus species infecting ivy gourd (coccinia grandis l.) in tamil nadu, india. | a distinct bipartite begomovirus was found to be associated with the mosaic disease on ivy gourd (coccinia grandis l.) in tamil nadu, india. the complete dna a and dna b components were cloned by rolling circle amplification. genome organization of this virus is found to be typical of old world bipartite begomovirus. the association of betasatellite component with this virus is absent. the closest nucleotide identity of 73.4 % was seen with the loofa yellow mosaic virus (lymv-[vn]-af509739) sugg ... | 2016 | 26739457 |
| a bemisia tabaci midgut protein interacts with begomoviruses and plays a role in virus transmission. | begomoviruses are a major group of plant viruses, transmitted exclusively by bemisia tabaci (gennadius) in a persistent circulative non-propagative manner. the information regarding molecular and cellular basis underlying begomovirus - whitefly interaction is very scarce. evidences have suggested that the insect gut possesses some crucial protein receptors that allow specific entry of virus into the insect haemolymph. we have performed yeast two hybrid gut cdna expression library screening again ... | 2016 | 26488331 |
| molecular characterization of a first begomovirus associated with lentil (lens culinaris) from india. | a disease of lentil with symptoms of distortion, mottling and chlorosis in the leaves, shortening of internodes and excessive branching was noticed in lentil at kanpur, india, during 2012-2014. results of polymerase chain reaction and reverse transcriptase polymerase chain reaction employed to detect suspected rna and dna viruses indicated involvement of a geminivirus, which was further characterized by sequencing of full genome amplified by rolling circle amplification. analysis of full length ... | 2017 | 27640431 |
| molecular detection and partial characterization of a begomovirus causing leaf curl disease of potato in sub-himalayan west bengal, india. | the characteristic disease symptoms of apical leaf curl, crinkled leaves and conspicuous mosaic were observed in potato plants grown in areas of coochbehar, one of the distinct agroclimatic zones of sub-himalayan west bengal. whitefly (bemisia tabaci) population was also observed in and around the infected plants. the characteristic disease symptoms and presence of whitefly (bemisia tabaci) population indicated the possibility of begomovirus infection. total dna was extracted from infected sampl ... | 2014 | 24813019 |
| uniplex and duplex pcr detection of geminivirus associated with potato apical leaf curl disease in india. | apical leaf curl disease has emerged as a new disease in potato during the last decade in india due to a change in planting date and an increased whitefly population. its incidence is on the rise threatening the cultivation of potato across the country. hence, a pcr assay was developed for the detection of tomato leaf curl new delhi virus-potato (tolcndv-potato) which is the causal agent of apical leaf curl disease in potato. primers specific to the coat protein (av1) and replicase (ac1) gene re ... | 2013 | 23711886 |
| role of betasatellite in the pathogenesis of a bipartite begomovirus affecting tomato in india. | betasatellites are commonly associated with tomato leaf curl disease caused by begomoviruses in india. this study demonstrates the role of a betasatellite in the pathogenesis of tomato leaf curl new delhi virus (tolcndv), a bipartite begomovirus affecting tomato in india. for infection, accumulation, systemic movement and disease induction by tolcndv, co-infection by the associated betasatellite was not essential, as the dna a alone of tolcndv could infect tomato and nicotiana benthamiana and in ... | 2012 | 22418785 |