| genetic diversity and population structure of rice stripe virus in china. | rice stripe virus (rsv) is one of the most economically important pathogens of rice and is repeatedly epidemic in china, japan and korea. the most recent outbreak of rsv in eastern china in 2000 caused significant losses and raised serious concerns. in this paper, we provide a genotyping profile of rsv field isolates and describe the population structure of rsv in china, based on the nucleotide sequences of isolates collected from different geographical regions during 1997-2004. rsv isolates cou ... | 2009 | 19264655 |
| [sequence aanlysis of intergenic region of rice stripe virus rna4: evidence for mixed infection and genomic variation]. | the intergenic region (ir) of the rna4 of 22 isolates of rice stripe virus (rsv) in china was cloned and sequenced. the ir sequences were compared with one another and with that from japan. sequence comparisons showed that these isolates could be divided into three different types, with the ir length of 634 bp, 654 bp and 732 bp, respectively. it is interesting to note three different types all occurred in yunnan rsv natural population, whereas other province only existed 654 bp type length isol ... | 2003 | 16281554 |
| [sequence analysis of rna3 of rice stripe virus isolates found in china: evidence for reassortment in tenuivirus]. | the rna3 segments of four isolates of rice stripe virus (rsv), isolated from endemic sites at panjin (pj), liaoning province, kunming (km) and yiliang (yl), yunnan province, as well as from outbreak sites at hongze (hz), jiangsu province, were determined. rna3 of these four isolates were 2480 bp, 2509 bp, 2489 bp and 2497 bp in length, respectively. compared with rna3 of t and m isolates from japan and y isolate from yunnan province of china, that had been previously reported, these seven isolat ... | 2003 | 12518236 |
| evolution of rice stripe virus. | rice stripe virus (rsv) is an insect-borne tenuivirus of economical significance. it is endemic to the rice-growing regions of east asia and exhibits more genetic diversity in yunnan province of china. to gain more insights into the molecular epidemiology and evolution of rsv, recombination analyses were conducted and potential events were detected in each of the four rna segments of rsv. bayesian coalescent method was then applied to the time-stamped coding sequences of the cp gene. the nucleot ... | 2017 | 28189616 |
| [cloning and sequencing analysis of rice stripe virus genome segment 4 of chinese isolate y]. | the cdna fragment covering full-length sequence of rstv rna4 of yunnan isolate in china was obtained by rt-pcr. the pcr-derived fragment was then cloned into vector pcrii. the cloned cdna was sequenced. comparison of the nucleotide and deduced amino acid sequences with those of the japanese isolate t was made. the results showed that at the nucleotides level, vorf, vcorf and the intergenic region had 94.9%, 94.1% and 86.1% identity respectively, the 5'-untranslational region was exactly the same ... | 1999 | 12555399 |
| genetic variability and evolution of rice stripe virus. | rice stripe virus (rsv) is the type member of the genus tenuivirus. rsv is known to have four segmented, single-stranded rna molecules and causes rice stripe disease in the rice fields of china, japan, and korea. based on the complete genomic sequences of the determined 6 rsv isolates (from yunnan, jiangsu, zhejiang, and liaoning provinces, china) and 27 other rsv isolates (from yunnan, jiangsu, anhui, henan, and shandong provinces of china, also japan and korea) downloaded from genbank, we prov ... | 2013 | 24101204 |
| [sequence analysis of ran4 of a severe isolate of rice stripe virus in china]. | rna4 segment of a severe isolate of rice stripe virus, isolated from liaoning province and designated as pj isolate, was amplified, cloned and sequenced. rna4 of pj isolate had 2157 nucleotide in length. when compared with rna4 of t and m isolates of japan and cx isolate of yunnan province of china that had been previously reported, we found that these four isolates could be divided into two groups. pj, t and m isolates shared 97.0% and 97.0%-97.5% identities in vorf4 and vcofr4 at the nucleotid ... | 2001 | 12549184 |