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[human coronavirus-nl63 was detected in specimens from children with acute respiratory infection in beijing, china].a new human coronavirus, hcov-nl63, was identified recently from two dutch children with acute respiratory infection (ari) by two scientists in the netherlands in 2004. to investigate if this newly discovered virus is associated with acute respiratory infections in pediatric patients in beijing, tests were developed to detect hcov-nl63 gene fragments from throat swab and nasopharyngeal aspirates collected from children in outpatient and inpatient departments with ari in beijing from dec. 2003 to ...200616624059
[sequence analysis for genes encoding nucleoprotein and envelope protein of a new human coronavirus nl63 identified from a pediatric patient in beijing by bioinformatics].the aim of this study was to characterize the n and e protein encoding genes of a new human coronavirus (hcov-nl63) which was identified from one of the clinical specimens (bj8081) collected from a 12 years-old patient with acute respiratory infection in beijing. the complete n and e gene sequences of hcov-nl63 were amplified from clinical sample by rt-pcr, then were cloned into the pcf-t and pucm-t vectors respectively and sequenced. the complete sequences of n and e genes were submitted to gen ...200717894224
characterization and complete genome sequence of human coronavirus nl63 isolated in china.human coronavirus nl63 (hcov-nl63) was first discovered in amsterdam in 2004 and was identified as a new human respiratory coronavirus. we here report the first complete genome sequence of hcov-nl63 strain cbj 037 isolated in 2008 from a patient with bronchitis in beijing, china.201222879617
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