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trends of brucellosis in florida. an epidemiologic review.human brucellosis in florida is documented for the 47-year period 1928-1975. of the 936 cases reported in 1930-1975, more than half (505) occurred in the decade 1940-1949. the incidence declined rapidly to an average rate of five cases per year, although there was an increase in 1974 and 1975. the analysis is mainly concerned with the years 1961-1975, since more complete epidemiologic data are available for this period. cases occurred throughout the year, with the highest incidence being between ...1977403760
brucellosis in feral swine in florida.serums from 95 feral swine trapped in glades county, florida, were tested for brucella antibodies, using the standard tube, card, rivanol, and complement-fixation tests. fifty (53%) of the animals were seropositive to at least 1 test. the standard tube test detected 98% of the serums positive to any of the 4 procedures. the prevalence of brucella antibodies was significantly lower (p less than 0.0005) in feral swine less than 6 months old than in those greater than or equal to 6 months old. the ...1978738942
incidence of antibody for hemagglutinating encephalomyelitis virus in serums from swine in the united states.serums prepared from blood collected from swine in arizona, florida, georgia, hawaii, iowa, maine, new hampshire, oregon, vermont, and washington were examined by the hemagglutination-inhibition (hi) test for antibody to hemagglutinating encephalomyelitis virus (hev). antibody was detected in serums from swine in each of the states except maine, new hampshire, and vermont. the frequency of antibody ranged from 10.9% (6 of 55) with serums from swine in oregon, to 98.6% (71 of 72) with serums from ...1975807134
antibodies to vesicular stomatitis virus in populations of feral swine in the united states.from 1979 to 1985, 941 feral swine (sus scrofa) from 53 locations in 15 states were serologically tested for antibodies to vesicular stomatitis virus (vsv). antibodies to new jersey serotype vsv were present in 75 swine from five locations in arkansas, florida, georgia, and louisiana. within these populations, antibody prevalences ranged from 10 to 100%. no antibodies to indiana serotype were detected.19863016348
parasites, diseases and health status of sympatric populations of sambar deer and white-tailed deer in florida.from december 1983 to december 1984 a study on parasites, diseases and health status was conducted on sympatric populations of sambar deer (cervus unicolor) and white-tailed deer (odocoileus virginianus) from st. vincent island, franklin county, florida. ten sambar and six white-tailed deer were examined. white-tailed deer had antibodies to epizootic hemorrhagic disease virus and bluetongue virus. serologic tests for antibodies to the etiologic agents of bovine virus diarrhea, infectious bovine ...19873035242
phage typing of the mycobacterium avium-intracellulare-scrofulaceum complex. a study of strains of diverse geographic and host origin.a total of 339 strains of the mycobacterium avium-intracellulare-scrofulaceum complex were phage typed using our previously described technique and 11 typing phages. these included 235 strains of human origin obtained from state health laboratories in virginia, georgia, florida, and arkansas, 26 strains isolated from persons with aids, 38 strains isolated from animals, and 40 environmental isolates. a phage-typing scheme was developed that denotes sensitivity to 8 primary typing phages: the jf g ...19854026062
encephalomyocarditis virus infections in florida, 1960-1966. 19674293832
high mortality in a florida swine herd infected with the encephalomyocarditis virus. an accompanying epizootiologic survey. 19684297718
host-feeding patterns of florida mosquitoes. i. aedes, anopheles, coquillettidia, mansonia and psorophora. 19714403447
the occurrence and effects of the local strains of the california encephalitis group of viruses in domestic mammals of florida. 19734745239
a comparative study of virulences, plaque morphologies and antigenic characteristics of venezuelan encephalitis virus strains. 19694976122
demodectic mange of swine. 19715166924
tamiami virus, a new member of the tacaribe group. 19705446318
tensaw virus, a new member of the bunyamwera arbovirus group from the southern united states. 19695764201
african swine fever--an assessment of risk for florida. 19846725096
ixodid ticks on feral swine in florida.more than 99% of the 645 feral swine (sus scrofa l.) in southern florida harbored ixodid ticks. dermacentor variabilis (american dog tick) was present on 99.6% of the swine and comprised 82.5% of the ticks collected. amblyomma maculatum (gulf coast tick) occurred on 85.9% of the hosts and 17.4% of the collections were of this species. amblyomma americanum (lone star tick) and ixodes scapularis (black-legged tick) were found infrequently and together constituted less than 0.1% of the ticks. pigs ...19846737605
prevalence and distribution of sarcocystis sp among wild swine of southeastern united states.digestive techniques revealed sarcocystis sp infection in 62 of 192 (32%) wild swine collected in alabama, arkansas, georgia, louisiana, mississippi, north carolina, south carolina, tennessee, virginia, and west virginia. sarcosporidia were not detected in wild swine of florida and were rarely found in the lower coastal plains of georgia, mississippi, and south carolina. swine from mountainous terrain in north carolina, tennessee, and west virginia and bottomland floodplain regions of alabama, a ...19816799467
induced african swine fever in feral pigs.feral pigs trapped in florida were exposed by intranasal/oral inoculation or contact to african swine fever virus isolants from the iberian peninsula and the dominican republic. all exposed pigs became sick and died or were killed after becoming moribund. necropsy revealed lesions typical of african swine fever, but several pigs had marked hemorrhage surrounding the kidneys or rectum or in the gastric, hepatic, or ventral abdominal region that, in the field, might have suggested trauma or poison ...19817309606
prevalence of brucella sp. antibodies in feral swine in florida.serum samples collected from feral swine (sus scrofa) throughout florida (usa) from 1974 to 1989 were tested for antibodies to brucella sp. by the card test, the standard tube test, the rivanol test or the complement fixation test. seropositive swine were detected at six of 18 sites with a composite prevalence of 23.4% (238 of 1,015 samples; range = 5.5% to 33.3%) for sites with seropositive swine. at one site for which age and sex data were available there was no significant difference (p = 0.5 ...19938355342
myocarditis in mice and guinea pigs experimentally infected with a canine-origin borrelia isolate from florida.to characterize the pathogenic potential of a unique borrelia isolate obtained from a dog from florida (fcb isolate).19968712515
genital infection and transmission of pseudorabies virus in feral swine in florida, usa.seventeen feral swine (fs) naturally infected with pseudorabies virus (prv) and treated with dexamethasone (4 mg/kg body wt) on five consecutive days shed virus primarily from the genital tract and less frequently from the upper respiratory tract. the fs isolates were identified as prv by virus neutralization with specific polyclonal antiserum and by direct immunofluorescence. restriction endonuclease analysis with bamhi showed that representative samples from a total of 62 isolates were identic ...19979220605
classification of antibiotic resistance patterns of indicator bacteria by discriminant analysis: use in predicting the source of fecal contamination in subtropical waters.the antibiotic resistance patterns of fecal streptococci and fecal coliforms isolated from domestic wastewater and animal feces were determined using a battery of antibiotics (amoxicillin, ampicillin, cephalothin, chlortetracycline, oxytetracycline, tetracycline, erythromycin, streptomycin, and vancomycin) at four concentrations each. the sources of animal feces included wild birds, cattle, chickens, dogs, pigs, and raccoons. antibiotic resistance patterns of fecal streptococci and fecal colifor ...200010966379
prevalence of sparganosis by county of origin in florida feral swine.sparganosis is a parasitic infection in amphibians, reptiles and mammals including feral swine and man. it is caused by migration of the metacestode (spargana) of spirometra. the primary objective of this study was the determination of the prevalence of gross sparganosis in florida county of origin in slaughtered feral swine. tracebacks to county of origin were conducted for florida feral swine with and without gross sparganosis. feral swine trapped in florida and presented for slaughter in a te ...200111390076
ixodid ticks on white-tailed deer and feral swine in florida.a state-wide survey was conducted in florida during the 1997-99 hunting seasons to examine white-tailed deer (odocoileus virginianus) and feral swine (sus scrofa) for potential indigenous vectors of the rickettsial agent of heartwater, cowdria ruminantium. a total of 504 white-tailed deer and 166 feral swine was examined from 30 wildlife management areas across the state. amblyomma maculatum, an experimental vector of c. ruminantium, was common on both deer and feral swine throughout the state. ...200111469190
isolation and characterization of a virus from soft ticks (ornithodoros capensis group) collected on bush key, dry tortugas, florida. 196414106042
geographical variation in antibiotic resistance profiles of escherichia coli isolated from swine, poultry, beef and dairy cattle farm water retention ponds in florida.the aim of this study was to assess geographical variation in multiple antibiotic resistance (mar) profiles of livestock escherichia coli as well as to evaluate the ability of mar profiles to differentiate sources of faecal pollution.200616405684
efficacy of four trap types for detecting and monitoring culex spp. in north central florida.the effectiveness of four types of mosquito traps at sampling culex mosquitoes was compared at two north central florida study sites (a commercial dairy and a swine research unit) with highly eutrophic lagoons that have a history of producing large populations of culex mosquitoes. traps evaluated included a john hock (jh) model 1012 cdc style light trap, jh model 1712 cdc gravid trap (gt), and american biophysics corporation's mosquito magnet-experimental (mm-x) and commercial model mm-professio ...200617162943
brucella suis infection associated with feral swine hunting - three states, 2007-2008.historically, brucellosis from brucella suis infection occurred among workers in swine slaughterhouses. in 1972, the u.s. department of agriculture national brucellosis eradication program was expanded to cover swine herds. subsequent elimination of brucellosis in commercial swine resulted in a decrease in b. suis-associated illness in humans. currently, swine-associated brucellosis in humans in the united states is predominantly associated with exposure to infected feral swine (i.e., wild boar ...200919521334
antibodies to west nile virus in feral swine from florida, georgia, and texas, usa.west nile virus (wnv) exposure has not yet been reported in feral swine (sus scrofa) despite the broad geographic range and population density of this species. the objectives of this study were to determine the prevalence of antibodies to wnv in feral pigs, and to evaluate serologic diagnostics as applied to this species. feral pig serum from three states was evaluated for antibodies to wnv. the overall wnv seroprevalence rate for 222 samples collected in 2001-2004 was 22.5%. seroprevalence rate ...200616989565
immunocontraception of florida feral swine with a single-dose gnrh vaccine.methods to limit fertility of feral swine are needed to reduce transmission of diseases and agricultural and ecosystem damage. method of study we evaluated a single-shot gnrh immunocontraceptive vaccine in both male and female feral swine for its effect on fertility and functional status of the reproductive tissues. captive feral pigs were randomly assigned to receive 1000 or 2000 microg gnrh-klh vaccine treatments or no treatment.200616635212
variation of total mercury concentrations in pig frogs (rana grylio) across the florida everglades, usa.the pig frog (rana grylio) is an aquatic frog that is an abundant component of the everglades ecosystem. south floridians recreationally and commercially hunt pig frogs in marshes throughout water conservation areas (wca) and big cypress national preserve (bcnp) in south florida. most of these areas are under fish consumption advisories because of high levels of methylmercury present in game fish tissues. it is important to understand how mercury is distributed throughout pig frog populations be ...200415919527
presence of encysted immature nematodes in a released whooping crane (grus americana).numerous nematode cysts were observed throughout the mesentery and on the surface of gastrointestinal organs in a whooping crane (grus americana) that was found dead in a central florida marsh. morphology of the excysted nematodes most closely resembled third-stage larvae in the order spirurida but were not similar to any species previously reported in whooping cranes. evidence presented suggests that the larvae may be physocephalus sexalatus, a swine spirurid in the subfamily ascaropsinae that ...200112785711
geographical variation in ribotype profiles of escherichia coli isolates from humans, swine, poultry, beef, and dairy cattle in florida.waters impacted by fecal pollution can exact high risks to human health and can result in financial losses due to closures of water systems used for recreation and for harvesting seafood. identifying the sources of fecal pollution in water is paramount in assessing the potential human health risks involved as well as in assessing necessary remedial action. recently, various researchers have used the ribotyping method to identify sources of bacterial indicators (escherichia coli and enterococci) ...200312571033
campaign to outlaw sow housing in florida advances. 200212369682
organochlorine compounds in florida feral pigs (sus scofa). 200010757657
sparganosis in feral hogs (sus scrofa) from florida. 199910416473
outbreak of staphylococcal food poisoning associated with precooked ham--florida, 1997.on september 27, 1997, a community hospital in northeastern florida notified the st. johns county health department about several persons who were treated in the emergency department because of gastrointestinal illnesses suspected of being associated with a common meal. this report summarizes the investigation of the outbreak by the florida department of health; the findings implicated staphylococcal intoxication as the cause of illness among some persons who attended a retirement party on septe ...19979414148
prevalence of pseudorabies (aujeszky's disease) virus antibodies in feral swine in florida.serum samples collected from feral swine (sus scrofa) throughout florida (usa) from 1980 to 1989 were tested for antibodies to pseudorabies virus (prv) by the serum neutralization test, the latex agglutination test, or by the enzyme-linked immunosorbent assay. seropositive swine were detected at 11 of 13 sites with a composite seroprevalence of 34.8% (579 of 1,662 samples; range = 5.9% to 58.2%) for sites with seropositive swine. data on age and sex of the swine were available from three sites. ...19938394943
brucellosis in wild swine: a serologic and bacteriologic survey in the southeastern united states and hawaii.sera from wild swine from 20 populations in 11 southeastern states and hawaii were tested for brucellosis by the card, tube, plate, buffered plate antigen, complement-fixation, and rivanol tests. twenty-one of 352 (6.0%) swine were considered seropositive, and these animals originated from 7 populations in 5 states: arkansas, florida, georgia, louisiana, and south carolina. cultural examinations for brucella suis also were made from subsamples of the swine tested serologically. brucella suis bio ...19827174444
lungworms of feral swine in florida.metastrongylus apri, m salmi, and m pudendotectus were found in 94%, 76%, and 64%, respectively, of 90 feral swine from a study area in southern florida. the mean number of worms per infected animal was 155, with a range of 1-1,980 (1 or more species). metastrongylus apri was found singly or in combination with 1 or both of the other species. single or concurrent infections of m salmi and m pudendotectus were not found. male hogs had significantly (p less than 0.05) higher intensities of m apri ...19827174442
coccidia of feral swine from florida.fecal samples from 251 free-ranging feral swine (sus scrofa) in florida were examined for coccidia. ten species of coccidia were recovered. in descending order with respect to prevalence, they were eimeria porci, e debliecki, e neodebliecki, e scarbra, e suis, e cerdonis, e polita, e perminuta, isospora suis, and e spinosa. of the swine examined, 87% were passing oocysts and 82% of the infected individuals were shedding at least 2 species of coccidia. all age classes included infected individual ...19827174441
tamiami (tam) strain. w-10777. 19704395318
two successive outbreaks of clostridium perfringens at a state correctional institution.an outbreak of acute gastrointestinal illness of short duration involving 100 inmates at a correctional institution followed a similar outbreak among the same population by eight days. clostridium perfringens was the specific etiology in both outbreaks; the vehicle was roast beef in the first outbreak, ham in the second. direct observation of food handling practices revealed that the meats were not cooled quickly enough following cooking; not reheated adequately prior to serving, and; held at im ...19852858163
serologic survey for transmissible gastroenteritis virus neutralizing antibodies in selected feral and domestic swine sera in the southern united states.serum samples collected from feral and domestic swine (sus scrofa) in florida and feral swine in georgia and texas were assayed by plaque reduction for their virus neutralizing (vn) antibodies against the porcine transmissible gastroenteritis virus (tge). none of 560 samples collected from feral swine contained vn antibodies for tge virus, but experimentally infected feral swine seroconverted. none of 665 samples from domestic swine contained tge-vn antibodies. these results indicate feral swine ...19902167395
minimizing elisa background in the diagnosis of swine trichinellosis.after confirming that long-term serum storage (frozen at -20 c for greater than 3 mo) causes optical density to drift upward, several modifications of an enzyme-linked immunosorbent assay (elisa) protocol were evaluated to identify a protocol that would reduce background in porcine sera tested for trichinellosis. modifications evaluated included blocking the antigen-coated elisa plate with sample diluent containing 10% bovine serum albumin (bsa) or 10% nonfat milk powder (bovine lacto transfer o ...19921403424
cytokine protein expression levels in tracheobronchial lymph node homogenates of pigs infected with pseudorabies virus.pseudorabies virus (prv) is a neurotropic alphaherpesvirus that produces fatal encephalitis in newborn pigs, respiratory disorders in fattening pigs, and reproductive failure in sows. following primary infection of the respiratory tract, prv can develop into a systemic infection with dispersion of the virus via the lymphatic system that involves mononuclear cells in tracheobronchial lymph nodes (tblns). the objectives of the present study were to evaluate the pathogenesis and to determine the ea ...201020219878
detection and characterization of avian influenza and other avian paramyxoviruses from wild waterfowl in parts of the southeastern united states.cloacal swabs were taken from migratory hunter-killed, nonmigratory, nesting waterfowl and migratory shorebirds from wildlife refuges in alabama, georgia, and florida during 2006 to 2008. samples were processed in embryonated eggs followed by hemagglutination (ha), directigen, and real-time reverse transcription-pcr tests. sequence analysis of the hemagglutinin (h) gene of the h10n7 alabama isolate revealed that it was closely related (98%) to recent isolates from delaware and canada, but only 9 ...200919276434
kempopeptins a and b, serine protease inhibitors with different selectivity profiles from a marine cyanobacterium, lyngbya sp.two cyclodepsipeptides named kempopeptins a (1) and b (2) were isolated from a collection of a floridian marine cyanobacterium, lyngbya sp., that had previously afforded the structurally related potent elastase inhibitors lyngbyastatin 7 and somamide b. the structures of 1 and 2 were elucidated mainly by 1d and 2d nmr spectroscopy, and the absolute configuration was established by chiral hplc and marfey's analysis of the degradation products. kempopeptin a (1) exhibited an ic50 against elastase ...200818693761
influenza exposure in united states feral swine populations.swine play an important role in the disease ecology of influenza. having cellular receptors in common with birds and humans, swine provide opportunities for mixed infections and potential for genetic reassortment between avian, human, and porcine influenza. feral swine populations are rapidly expanding in both numbers and range and are increasingly coming into contact with waterfowl, humans, and agricultural operations. in this study, over 875 feral swine were sampled from six states across the ...200818436668
thermoregulation in larval aggregations of carrion-feeding blow flies (diptera: calliphoridae).the growth and development of carrion-feeding calliphorid (diptera: calliphoridae) larvae, or maggots, is of great interest to forensic sciences, especially for estimation of a postmortem interval (pmi). the development rate of calliphorid larvae is influenced by the temperature of their immediate environment. heat generation in larval feeding aggregations (=maggot masses) is a well-known phenomenon, but it has not been quantitatively described. calculated development rates that do not include i ...200717547240
forensically important calliphoridae (diptera) associated with pig carrion in rural north-central florida.a study to determine the relative abundance and seasonality of forensically important blow flies (diptera: calliphoridae) in rural north-central florida was conducted using pig carcasses (sus scrofa l.) as models for human bodies. seven species of calliphoridae were collected: lucilia coeruleiviridis (=phaenicia) (macquart), cochliomyia macellaria (f.), chrysomya rufifaces (macquart), phormia regina (meigen), chrysomya megacephala (f.), and a few specimens of calliphora livida hall, and callipho ...200717547239
lyngbyastatin 4, a dolastatin 13 analogue with elastase and chymotrypsin inhibitory activity from the marine cyanobacterium lyngbya confervoides.lyngbyastatin 4 (1), a new depsipeptide containing the unusual amino acid homotyrosine and a 3-amino-6-hydroxy-2-piperidone (ahp) residue, was isolated from a collection of the marine cyanobacterium lyngbya confervoides off the florida atlantic coast. its gross structure was determined by nmr spectroscopy, and the configurations of asymmetric centers were assigned after chiral hplc analysis of hydrolysis products. lyngbyastatin 4 (1) is an analogue of the sea hare isolate dolastatin 13 and sever ...200717253864
the miami results on porcine islet-sertoli cell xenotransplantation. 200717214709
in vitro p-glycoprotein interactions and steady-state pharmacokinetic interactions between tolvaptan and digoxin in healthy subjects.interactions between tolvaptan and digoxin were determined in an open-label, sequential study where 14 healthy subjects received tolvaptan 60 mg once daily (qd) on days 1 and 12 to 16 and digoxin 0.25 mg qd on days 5 to 16. mean maximal concentrations (c(max)) and area under the curve during the dosing interval (auc(τ)) for digoxin with tolvaptan (day 16) were increased 1.27- and 1.18-fold compared with digoxin alone (day 11); mean renal clearance of digoxin was decreased by 59% (p < .05). tolva ...201120679500
applying social science and public health methods to community-based pandemic planning.pandemic influenza is a unique threat to communities, affecting schools, businesses, health facilities and individuals in ways not seen in other emergency events. this paper aims to outline a local government project which utilised public health and social science research methods to facilitate the creation of an emergency response plan for pandemic influenza coincidental to the early stages of the 2009 h1n1 ('swine flu') outbreak. a multi-disciplinary team coordinated the creation of a pandemic ...201021177224
contributions of an animal scientist to reproductive biology.i became interested in biology as an undergraduate in a pre-medical curriculum, but developed a passion for the field of reproductive biology because of a course in physiology of reproduction taken to meet requirements for admission to veterinary school. my career path changed and i entered graduate school, obtained the ph.d. and have enjoyed an academic career as a reproductive biologist conducting research in uterine biology and pregnancy in animal science departments at the university of flor ...201121565996
immune correlates of protection against influenza: challenges for licensure of seasonal and pandemic influenza vaccines, miami, fl, usa, march 1-3, 2010.the emergence of a novel swine-origin pandemic influenza virus in 2009, together with the continuing circulation of highly pathogenic avian h5n1 viruses and the urgent global need to produce effective vaccines against such public health threats, has prompted a renewed interest in improving our understanding of the immune correlates of protection against influenza. as new influenza vaccine technologies, including non-ha based approaches and novel production platforms are developed and undergo cli ...201121651740
high prevalence of trichinella pseudospiralis in florida panthers (puma concolor coryi).parasites of the genus trichinella are zoonotic nematodes common in carnivores throughout the world. we determined the prevalence and species of trichinella infections in florida panthers (puma concolor coryi).201525649739
an examination of the transition of fracture characteristics in long bones from fresh to dry in central florida: evaluating the timing of injury.it is important to conduct timing of injury research analyzing fracture characteristics at known postmortem intervals (pmi) because bone can retain fresh characteristics throughout the pmi. defleshed pig (sus scrofa) long bones were fractured weekly in two environments (full sun and shade) over 14 weeks in central florida and fracture characteristics were categorized (n = 136) for analysis. results of analysis of variance (anova) using time in weeks (pmi) as a dependent variable indicate signifi ...201727864956
serosurvey of leptospirosis in feral hogs (sus scrofa) in florida.leptospira is a global pathogen of emerging public health importance in both developing and industrialized nations and can infect almost all mammalian species, including humans. as suburbanization and the popularity of outdoor recreational activities increases, so do human-wildlife and companion animal-wildlife interfaces. florida offers a tropical climate favorable for outdoor activities and a semirural landscape that sustains an abundant feral hog population. because no survey ofleptospirosis ...201323805559
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