| identification and characterization of citrus tristeza virus isolates breaking resistance in trifoliate orange in california. | most citrus tristeza virus (ctv) isolates in california are biologically mild and symptomless in commercial cultivars on ctv tolerant rootstocks. however, to better define california ctv isolates showing divergent serological and genetic profiles, selected isolates were subjected to deep sequencing of small rnas. full-length sequences were assembled, annotated and trifoliate orange resistance-breaking (rb) isolates of ctv were identified. phylogenetic relationships based on their full genomes pl ... | 2017 | 28453412 |
| development and validation of a multiplex reverse transcription quantitative pcr (rt-qpcr) assay for the rapid detection of citrus tristeza virus, citrus psorosis virus, and citrus leaf blotch virus. | a single real-time multiplex reverse transcription quantitative polymerase chain reaction (rt-qpcr) assay for the simultaneous detection of citrus tristeza virus (ctv), citrus psorosis virus (cpsv), and citrus leaf blotch virus (clbv) was developed and validated using three different fluorescently labeled minor groove binding qpcr probes. to increase the detection reliability, coat protein (cp) genes from large number of different isolates of ctv, cpsv and clbv were sequenced and a multiple sequ ... | 2015 | 25907469 |
| calculation of diagnostic parameters of advanced serological and molecular tissue-print methods for detection of citrus tristeza virus: a model for other plant pathogens. | citrus tristeza virus (ctv) is one of the most important virus diseases that affect citrus. control of ctv is achieved by grafting selected virus-free citrus scions onto ctv-tolerant or -resistant rootstocks. quarantine and certification programs are essential for avoiding the entry and propagation of severe strains of ctv. citrus nurseries in spain and central california (united states) maintain zero-tolerance policies for ctv that require sensitive, specific, and reliable pathogen-detection me ... | 2012 | 21879789 |
| genetic variation of citrus tristeza virus isolates from california and spain: evidence for mixed infections and recombination. | we examined the population structure and genetic variation of four genomic regions within and between 30 citrus tristeza virus (ctv) isolates from spain and california. our analyses showed that most isolates contained a population of sequence variants, with one being predominant. four isolates showed two major sequence variants in some genomic regions. the two major variants of three of these isolates showed very low nucleotide identity to each other but were very similar to those of other isola ... | 2001 | 11483750 |
| population structure and genetic diversity within california citrus tristeza virus (ctv) isolates. | the closterovirus, citrus tristeza virus (ctv) is an aphid-borne rna virus that is the causal agent of important worldwide economic losses in citrus. biological and molecular variation has been observed for many ctv isolates. in this work we detected and analyzed sequence variants (haplotypes) within individual ctv isolates. we studied the population structure of five california ctv isolates by single strand conformation polymorphism (sscp) analysis of four ctv genomic regions. also, we estimate ... | 2000 | 11129629 |