| evaluation of rice resistance to southern rice black-streaked dwarf virus and rice ragged stunt virus through combined field tests, quantitative real-time pcr, and proteome analysis. | diseases caused by southern rice black-streaked dwarf virus (srbsdv) and rice ragged stunt virus (rrsv) considerably decrease grain yield. therefore, determining rice cultivars with high resistance to srbsdv and rrsv is necessary. in this study, rice cultivars with high resistance to srbsdv and rrsv were evaluated through field trials in shidian and mangshi county, yunnan province, china. sybr green i-based quantitative real-time polymerase chain reaction (qrt-pcr) analysis was used to quantitat ... | 2017 | 28241456 |
| the development and application of a dot-elisa assay for diagnosis of southern rice black-streaked dwarf disease in the field. | outbreaks of the southern rice black-streaked dwarf virus (srbsdv) have caused significant crop losses in southern china in recent years, especially in 2010. there are no effective, quick and practicable methods for the diagnosis of rice dwarf disease that can be used in the field. traditional reverse transcription-polymerase chain reaction (rt-pcr) methodology is accurate but requires expensive reagents and instruments, as well as complex procedures that limit its applicability for field tests. ... | 2012 | 22355457 |