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using msa-2b as a molecular marker for genotyping mexican isolates of babesia bovis.variable merozoite surface antigens of babesia bovis are exposed glycoproteins having a role in erythrocyte invasion. members of this gene family include msa-1 and msa-2 (msa-2c, msa-2a(1), msa-2a(2) and msa-2b). to determine the sequence variation among b. bovis mexican isolates using msa-2b as a genetic marker, pcr amplicons corresponding to msa-2b were cloned and plasmids carrying the corresponding inserts were purified and sequenced. comparative analysis of nucleotide and deduced amino acid ...200919931189
a common high molecular weight antigen of babesia bovis isolates from mexico.cattle from an area of mexico endemic with babesia bovis infections have a dominant antibody response to a 152kda antigen of the tamaulipas strain of b. bovis. a mab termed pb/5, showing a specific reactivity to this 152kda antigen in western blots, was identified. the mab which reacted with the blunt end of b. bovis in an indirect fluorescent antibody test also reacted to a 152kda antigen in two other isolates (nuevo leon and yucatan), and a 175kda antigen in the huasteca b. bovis isolate from ...200010675745
management factors associated with babesia bovis seroprevalence in cattle from eastern yucatán, mexico.the effect of management on the seroprevalence of babesia bovis was studied in 399 bos indicus cattle 1-2 years old from 92 farms in the eastern yucatán, méxico. the management factors studied were: farm-type, production system, herd size, farm size, stocking density, vector control, dipping interval, type of dipping, type of acaricide and cattle introduction to the farm. a cross-sectional study was carried out (2-stage cluster sampling). the number of serum samples was proportionally distribute ...199910423779
bovine babesiosis in dual purpose calves in the state of yucatan, mexico.thirty-eight dual purpose calves in the 3 agricultural zones of the state of yucatan, mexico were monitored for antibodies to babesia bovis and b. bigemina from close to birth up to 12 months of age. the results showed that infection with both parasites is widespread throughout the 3 zones. many of the animals had produced antibody to both parasites by 6 months of age indicating high transmission rates resulting in enzootic stability for both parasites. any changes to agricultural practices in t ...19989719829
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