| nucleotide sequence and deduced amino acid sequence of the nonstructural proteins of dengue type 3 virus, bangkok genotype. | the nucleotide sequence of the nonstructural protein gene (1,610 bases) of dengue 3 virus (bangkok genotype; ch53489 isolated in 1973) has been determined in both forward and reverse directions. the pcr based cycle sequencing technic by the enzymatic method of sanger et al using a sequencing primer 5'-end labeled with gamma-32p-atp was the method of our choice for sequence analysis. two cdna templates were prepared by rt-pcr technique starting from the nucleotides 6,306-6,969 and 6,925-7,915 of ... | 1998 | 9886128 |
| highly conserved nucleotide sequence and its deduced amino acids of the 5'-noncoding region and the capsid protein of a bangkok isolate dengue-3 virus. | the dengue-3 virus genome encodes an uninterrupted open reading frame (orf) flanked by 5' and 3' non-coding regions. the order of proteins encoded in dengue-3 virus orf, as with other flaviviruses, is: cap 5'-c-prm-e-ns1-ns2a-ns2b-ns3-ns4a-ns4b-ns5-3'. the nucleotide sequence of the 5'-noncoding region and the capsid protein of dengue-3virus (a bangkok isolate: ch53489 isolated by usamc-afrims in 1973) has been analyzed in both forward and reverse directions. the pcr-based cycle sequencing techn ... | 2000 | 11414440 |
| molecular genetic relationship of the 3' untranslated region among thai dengue-3 virus, bangkok isolates, during 1973-2000. | dengue virus serotype 3 (denv-3) was associated with severe dengue epidemics in thailand during 1973-1999. we studied thai denv-3 viruses isolated from hospitalized children in bangkok with differing disease severity during that period. viruses were sequenced at their 5' and 3' untranslated regions (utrs), which are regions that play a pivotal role in viral replication. our results indicated that the primary sequences as well as the secondary structures at both ends of thai denv-3 viruses were h ... | 2009 | 19563250 |