| variation in dengue type 2 viruses isolated in bangkok during 1980. | dengue type-2 viruses isolated in metropolitan bangkok during 1980 (bangkok/80) were characterized by oligonucleotide fingerprinting, restriction enzyme (re) mapping and antigenic analysis using monoclonal antibody probes. of 10 isolates analysed by oligonucleotide fingerprinting, nine were very closely related, showing 72.5% to 91.4% oligonucleotide homology. one isolate (d80-141) produced a distinctly different fingerprint (55.7% to 58.0% homology) and was less related to other bangkok/80 deng ... | 1988 | 3351477 |
| effect of temperature on the vector efficiency of aedes aegypti for dengue 2 virus. | the effect of temperature on the ability of aedes aegypti to transmit dengue (den) 2 virus to rhesus monkeys was assessed as a possible explanation for the seasonal variation in the incidence of dengue hemorrhagic fever in bangkok, thailand. in two laboratory experiments, a bangkok strain of ae. aegypti was allowed to feed upon viremic monkeys infected with den-2 virus. blood-engorged mosquitoes were separated into two groups and retained at constant temperatures. virus infection and transmissio ... | 1987 | 3812879 |
| comparative nucleotide and deduced amino acid sequence of the envelope glycoprotein gene among three dengue virus type 2 strains isolated from patients with different disease severities in maha sarakham, northeast thailand. | the nucleotide (nt) sequence of the envelope glycoprotein (e) gene of dengue virus type 2 was determined by the primer-extension dideoxy chain-termination method for 3 dengue virus type 2 (d2) strains which had been isolated from patients with dengue fever (df), dengue hemorrhagic fever (dhf), and dengue shock syndrome (dss), in maha sarakham, northeast thailand, in 1986-1987. their nt sequences were essentially the same except for a single silent nt replacement in each dhf and dss strain compar ... | 1994 | 7855635 |
| possible occurrence of a genetic bottleneck in dengue serotype 2 viruses between the 1980 and 1987 epidemic seasons in bangkok, thailand. | cocirculation of two genetic subtypes of dengue serotype 2 viruses was first observed in the 1980 epidemic season in thailand. to further delineate the evolutionary history and the contribution of these subtypes to subsequent epidemics, we determined the envelope glycoprotein gene sequence of 20 dengue serotype 2 viruses isolated from infected patients during 1987 and compared them with those derived from earlier years. subtype iiia strains represented the majority (18 of 19) of dengue type 2 vi ... | 1997 | 9242328 |
| molecular evolution of dengue type 2 virus in thailand. | dengue is a mosquito-borne viral infection that in recent years has become a major international public health concern. dengue hemorrhagic fever (dhf), first recognized in southeast asia in the 1950s, is today a leading cause of childhood death in many countries. the pathogenesis of this illness is poorly understood, mainly because there are no laboratory or animal models of disease. we have studied the genetic relationships of dengue viruses of serotype 2, one of four antigenically distinct den ... | 1998 | 9452299 |
| relationship between body size and severity of dengue hemorrhagic fever among children aged 0-14 years. | a hospital based case-control study was conducted from october 2002 to november 2003 among children aged 0-14 years at queen sirikit national institute of child health (children's hospital), bangkok, thailand. this study focused on body size and severity of dengue hemorrhagic fever (dhf) in children. one hundred five patients diagnosed as having dhf grade iii or iv were the cases and 105 diagnosed as having dhf grade i or ii were controls. they were matched at a ratio of 1:1 by their gender and ... | 2006 | 17124987 |
| evaluation of an ns1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness. | diagnosis of dengue infection during the febrile stage has been challenging. we evaluated the accuracy of ns1 protein detection in diagnosing dengue infection in patients presenting with acute febrile illness in bangkok, thailand. of the 235 subjects presented with fever of unknown source within 5 days, 132 (56.2%) were male with the median age of 17.8 (range, 3-52) years. the median duration of fever was 4 (range, 1-5) days. one hundred seventy-one (72.8%) patients had dengue infection, of whic ... | 2008 | 18191361 |