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multiple factors have shaped the phylogeography of chinese spiny loach cobitis sinensis in taiwan as inferred from mitochondrial dna variation.mitochondrial dna cytochrome b sequences (1140 bp) in 61 specimens of chinese spiny loach cobitis sinensis from 12 drainages in taiwan were identified as two major clades, exhibiting a southern and a northern distribution, north of tzengwen and south of tzengwen (including tzengwen), respectively. the divergence time between these two phylogroups was estimated at 7.34-9.06 million years before present (b.p.), but these two phylogroups were formed c. 3.41-4.23 and 2.22-2.75 m b.p., respectively. ...201020409169
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