| clinical impact of human metapneumovirus genotypes and genotype-specific seroprevalence in yamagata, japan. | the clinical impact of human metapneumovirus (hmpv) genotypes and the relation between the hmpv genotype in circulation and genotype-specific seroprevalence are yet to be clarified. we determined the genotypes of 93 hmpv strains that were isolated between 2004 and 2006 in yamagata, japan, and identified 35 genotype a2, 14 genotype b1, and 44 genotype b2 isolates. children infected with genotype a2 hmpv were significantly older than those infected with genotype b1 hmpv. diagnosis of laryngitis wa ... | 2008 | 18428137 |
| analysis of monthly isolation of respiratory viruses from children by cell culture using a microplate method: a two-year study from 2004 to 2005 in yamagata, japan. | although well over 200 viral agents have been implicated in acute respiratory infections (aris) among children, no system able to detect such a wide range of viruses has been established. between january 2004 and december 2005, a modified microplate method, including hef, hep-2, vero e6, mdck, rd-18s, and gmk cell lines (hhve6mrg plate), was adopted to isolate viruses. a total of 1,551 viruses were isolated, representing both outbreaks and sporadic cases, from 4,107 nasopharyngeal specimens, at ... | 2008 | 18503168 |
| endemicity of human metapneumovirus subgenogroups a2 and b2 in yamagata, japan, between 2004 and 2009. | to clarify a longitudinal epidemiology,we isolated 280 hmpv strains from patients with acute respiratory infections in yamagata, japan, between 2004 and 2009.we observed that the high season for hmpv was from winter to spring (between january and may) and the low season was in the fall (around september and october). a further molecular analysis revealed that subgenogroup a2 (a2) strains were the most commonly isolated (151/280; 53.9%), followed by b2 (108/280; 38.6%) and b1 (19/280; 6.8%). our ... | 2010 | 21140598 |
| seasonal patterns of respiratory syncytial virus, influenza a virus, human metapneumovirus, and parainfluenza virus type 3 infections on the basis of virus isolation data between 2004 and 2011 in yamagata, japan. | most acute respiratory infections (aris) are thought to be associated with respiratory viruses that cause similar symptoms. therefore, assessment of clinical and epidemiologic features of these viruses is important for diagnosing a viral infection. we collected 13,325 nasopharyngeal specimens from patients with aris and isolated the virus using a microplate method involving 7 cell lines between 2004 and 2011 in yamagata, japan. we isolated a total of 5,483 viruses. respiratory syncytial virus (r ... | 2013 | 23514911 |
| outbreak of human metapneumovirus detected by use of the vero e6 cell line in isolates collected in yamagata, japan, in 2004 and 2005. | a number of epidemiological studies have shown human metapneumovirus (hmpv) to be one of the most important viral agents associated with acute respiratory infections in humans. however, due to the difficulty in growing the virus, all epidemiological studies of hmpv infection have been performed on the basis of the molecular method. thus, the development of a cell line suitable for the isolation of hmpv from clinical specimens is a crucial step for further research. using the vero e6 cell line, w ... | 2007 | 17460056 |