| molecular evidence for the presence of new babesia species in feral raccoons (procyon lotor) in hokkaido, japan. | we recently reported that feral raccoons (procyon lotor) with splenomegaly native to japan were carriers of a babesia microti-like parasite identical to that found in the united states, which was likely introduced to japan from north america via raccoons imported as pets. thus, we attempted extensive molecular survey for piroplasma infections of feral raccoon with normal spleen in hokkaido, japan using nested pcr that target broadly to 18s ribosomal rna gene (ssu-rdna) of all the parasites in th ... | 2009 | 19349121 |
| human babesiosis in japan: epizootiologic survey of rodent reservoir and isolation of new type of babesia microti-like parasite. | we have carried out epizootiologic surveys at various sites in japan to investigate wild animals that serve as reservoirs for the agents of human babesiosis in the country. small mammals comprising six species, apodemus speciosus, apodemus argenteus, clethrionomys rufocanus, eothenomys smithii, crocidura dsinezumi, and sorex unguiculatus, were trapped at various places, including hokkaido, chiba, shiga, hyogo, shimane, and tokushima prefectures. animals harboring babesia microti-like parasites w ... | 2001 | 11724838 |
| survey of babesia microti infection in field rodents in japan: records of the kobe-type in new foci and findings of a new type related to the otsu-type. | of 247 rodents comprising 5 genera and 7 species collected at 17 sites throughout japan from 2003 to 2005, babesia microti was detected microscopically and by polymerase chain reaction (pcr) in 36 rodents comprising 2 genera and 3 species from 12 sites. based on the analysis of small subunit ribosomal rna gene (ssurdna) sequences, the kobe-type, the etiological type of the first japanese case of human infection was found in apodemus speciosus and apodemus argenteus in aomori, the northernmost pr ... | 2007 | 17237595 |
| babesia microti-like parasites detected in eurasian red squirrels (sciurus vulgaris orientis) in hokkaido, japan. | six eurasian red squirrels (sciurus vulgaris orientis), victims of road traffic found during 2002 and 2004 near the noppro forest park in ebetsu, hokkaido, japan, were examined for the presence of babesia parasites. three of the six squirrels exhibited positive signals by nested pcrs targeting both the 18s rrna and beta-tubulin genes. three squirrels proved to be infected with a b. microti-like parasite as evidenced by sequencing the amplified dnas and by the morphology of the intraerythrocytic ... | 2006 | 16891774 |
| babesia microti-like parasites detected in feral raccoons (procyon lotor) captured in hokkaido, japan. | raccoons (procyon lotor), which have recently become feral in japan, were examined for the presence of babesia microti-like parasites. out of 372 raccoons captured in the west-central part of hokkaido, 24 animals with splenomegaly were selected and tested by nested pcr targeting the babesial 18s rrna gene. b. microti-like parasites were detected in two of the 24 individuals, and their dna sequences were identical to that of the b. microti-like parasite reported from raccoons in the united states ... | 2005 | 16141672 |
| u.s.-type babesia microti isolated from small wild mammals in eastern hokkaido, japan. | our previous report demonstrated that small wild rodents in japan harbored two types of novel babesia microti-like parasites (kobe and hobetsu types), but not the type widely distributed throughout the temperate zones of north american and eurasian continents (u.s. type). in this study, we surveyed small wild mammals collected at various places in the northern part of japan, seeking for u.s.-type b. microti. a total of 197 small mammals comprising 10 species, apodemus speciosus, a. argenteus, cl ... | 2004 | 15353841 |
| detection of babesia microti-like parasite in filter paper-absorbed blood of wild rodents. | the first case of human babesiosis was reported in japan. the epidemiology of this disease in japanese nature remains unclear. in this study, 97 common field mice captured in hokkaido, japan, were examined. blood specimens absorbed onto filter papers were eluted and tested by nested pcr using specific primers for the b. microti nuclear small subunit rrna genome. twenty-three percent (11/47) of apodemus speciosus and four percent (2/50) of clethrionomys rufocanus were positive. the 159-bp primary ... | 2002 | 11913551 |
| dynamics, co-infections and characteristics of zoonotic tick-borne pathogens in hokkaido small mammals, japan. | many of the emerging infectious diseases originate in wildlife and many of them are caused by vector-borne pathogens. in japan, zoonotic tick-borne pathogens (tbps) are frequently detected in both ticks and wildlife. here, we studied the infection rates of potentially zoonotic species, including anaplasma, ehrlichia, neoehrlichia and babesia spp., in hokkaido's most abundant small mammals as they relate to variable extrinsic factors that might affect the infection rates of these pathogens. a tot ... | 2016 | 27166277 |
| detection of two zoonotic babesia microti lineages, the hobetsu and u.s. lineages, in two sympatric tick species, ixodes ovatus and ixodes persulcatus, respectively, in japan. | the species babesia microti, commonly found in rodents, demonstrates a high degree of genetic diversity. three lineages, u.s., kobe, and hobetsu, are known to have zoonotic potential, but their tick vector(s) in japan remains to be elucidated. we conducted a field investigation at nemuro on hokkaido island and at sumoto on awaji island, where up to two of the three lineages occur with similar frequencies in reservoirs. by flagging vegetation at these spots and surrounding areas, 4,010 ticks, com ... | 2012 | 22389378 |