| single step purification and characterization of a thermostable and calcium independent a-amylase from bacillus amyloliquifaciens tswk1-1 isolated from tulsi shyam hot spring reservoir, gujarat (india). | a thermophilic bacteria, identified and designated as bacillus amyloliquifaciens tswk1-1 (16s rrna gene sequence, genbank: gq121033), was isolated from a hot water reservoir located at tulsi shyam, gujarat, india. the optimum temperature and ph for amylase production were 50 °c and 7.0, respectively. the crude enzyme was partially purified by ammonium sulphate fractionation followed by dialysis. however, single step purification was achieved on phenyl sepharose 6ff affinity column with 45.71% yi ... | 2011 | 21352849 |