| [studies on the strain differences of schistosoma japonicum in the mainland of china. xi. enzyme linked immunoelectrotransfer blot analysis]. | antigens prepared from s. japonicum adult worms of different isolates i. e. anhui, hubei, guangxi, yunnan and sichuan by origin were analyzed by enzyme linked immunoelectrotransfer blot (eitb) probed with rabbit anti-snail antibody (figs 1,2). anti-sera against oncomelania h. hupensis from anhui, hubei, yunnan and sichuan localities were prepared separately and used in the tests. the eitb patterns were similar in s. j. isolates of anhui and hubei, and it was also the case among s. j. isolates of ... | 1992 | 1303328 |
| sensitivity of different isolates of schistosoma japonicum from china to praziquantel. | groups of c57bl inbred mice infected with each of the 4 different isolates, (anhui, hubei, sichuan and yunnan) of schistosoma japonicum from the mainland of china were treated with praziquantel (pzq) and the parasiticidal effects were compared. worm reduction rate was recorded to assess systematically the sensitivity of 4 different isolates to pzq in the mouse. three dosage-levels of pzq, ie 150, 230 and 310 mg/kg body weight in single doses were used. the worm development rates of control group ... | 1992 | 1439978 |
| characteristics of different isolates of schistosoma japonicum from china in the final hosts. | the present paper deals with the susceptibility of common laboratory animals, such as mouse, rat, hamster, jird, rabbit and rhesus monkey, to infection with different isolates of schistosoma japonicum in the mainland of china under laboratory conditions. with the exception of the rat, all the animals under study were permissive hosts for different isolates though their worm recovery rates varied. the mean body length of pair-worms of the yunnan isolate was considerably smaller than that of the a ... | 1991 | 1948285 |
| comparison of morphology, pathogenicity and drug response among three isolates of schistosoma japonicum in the mainland of china. | geographic differences among isolates of schistosoma japonicum from anhui province (a) in the east, guangxi autonomous region (g) in the south and yunnan province (y) in the southwest of mainland of china were studied. c57bl mice were used to compare these isolates. morphologically, both male and female worms of isolate y were shorter than those of isolates a and g, and the ovary of female worm of isolate g was the smallest. in isolate a, the percentage of male worms bearing 7 testes was 87%, wh ... | 1989 | 2735700 |
| strain complex of schistosoma japonicum in the mainland of china. | the present paper deals with studies on the characteristics of schistosoma japonicum isolated from five localities in the mainland of china. the following items were observed and compared including morphometric data, susceptibility of six mammalian hosts, prepatent period, compatibility between larvae and snail hosts, size of hepatic granuloma produced by eggs, immunoreactions in experimental animals, sensitivity to praziquantel, sds-page protein pattern and its antigenicity analysis, dna hybrid ... | 1994 | 7855634 |
| revisiting the question of limited genetic variation within schistosoma japonicum. | recent electrophoretic data have indicated that schistosoma japonicum in mainland china may be a species complex, with the existence of a cryptic species being predicted from the analysis of schistosome populations from sichuan province. to investigate the sichuan form of s. japonicum, 4.9 kbp of mitochondrial dna from each of three samples of the parasite from china (two from sichuan and one from hunan) and one from sorsogon in the philippines were amplified, sequenced and characterized. the se ... | 2002 | 12080976 |
| an electrophoretic comparison of schistosoma japonicum (trematoda) from different provinces in the people's republic of china suggests the existence of cryptic species. | schistosoma japonicum from the people's republic of china is considered to represent a single species comprising either 1 or 4 'strains'. we conducted an allozyme electrophoretic study to examine the extent of genetic variation in s. japonicum from mainland china. the allelic profiles of s. japonicum from 7 provinces were established at 16 enzyme loci. s. japonicum from sichuan had 3-5 (19-31%) fixed differences compared with those from zhejiang, anhui, jiangxi, hunan, hubei and yunnan, suggesti ... | 1999 | 10581616 |
| [potential risks for transmission of schistosomiasis caused by mobile population in shanghai]. | to understand the potential risk for schistosomiasis transmission caused by introduction of infection source from mobile population in shanghai. | 2007 | 18038772 |
| use of rapd for the detection of genetic variation in the human blood fluke, schistosoma japonicum, from mainland china. | a random amplified polymorphic dna (rapd) technique using 16 decamer oligonucleotide primers was employed to characterize isolates of schistosoma japonicum from seven geographical locations (sj1: zhejiang; sj2: anhui; sj3: jiangxi; sj4: hunan; sj5: hubei; sj6: sichuan; sj7: yunnan) of the people's republic of china. distinct differences between some isolates were reproducibly detected in rapd patterns produced using five of the primers. the analyses showed that both sj6 and sj7 were quite distin ... | 1996 | 8910890 |
| sds-page protein pattern and its antigenicity analysis of different isolates of schistosoma japonicum in china. | homogenates prepared from s. japonicum adult worms of different isolates from anhui, hubei, guangxi, yunnan and sichuan provinces were analyzed by sds-page and enzyme linked immunoelectrotransfer blot (eitb) tested with rabbit anti-snails antibody. the results of sds-page indicated that with silver staining both male and female worms of guangxi isolate showed some definite differences in their protein profile, namely, absence of one band between 50-75 kda in male worms and marked reduction in qu ... | 1994 | 8187569 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. xiii. conclusion]. | up to the present, schistosoma japonicum endemic in the mainland of china has used to be considered being of only one strain, chinese mainland strain. it is well known that the endemic areas of schistosomiasis japonica in the chinese mainland are mainly confined to regions south of yangtze river, but they are discontinuous, showing conspicuous geographic and topographic isolation. so far, little is known about the characteristics of schistosomes isolated from various topographic areas. we have t ... | 1993 | 8174222 |
| [study on allele frequency in oncomelania from the mainland of china]. | nine populations of oncomelania, field-collected from anhui, shanghai, jiangsu, zhejiang, jiangxi, hunan, hubei, sichuan and yunnan were studied by horizontal starch gel electrophoretic method with 24 enzyme systems (aat, acph, ak, ao, aph, ck, est, gdh, gpi, g6pd, hbd, isdh, lap, ldh, me, mdh, mpi, nadd, oct, pgm, 6pgd, sdh, sod, xdh) analyzed. 40 loci and 117 alleles were detected in the oncomelania. both of gpi and pgm-i, with 7 alleles, were the most variable loci. 22 loci had more than 3 al ... | 1994 | 7867149 |
| morbidity in schistosomiasis japonica in relation to intensity of infection. a study of two rural brigades in anhui province, china. | schistosomiasis japonica remains endemic in several provinces south of the yangtze river in china because of relatively sparse populations of human beings and dense populations of snails. we studied two brigades in a rural commune in gui-chi county, anhui province, to determine the prevalence, intensity, and morbidity associated with this infection before concerted control efforts were instituted. quantitative fecal examinations, histories, and physical examinations relevant to schistosomiasis j ... | 1983 | 6656848 |
| failure to induce resistance of schistosoma japonicum to praziquantel. | in order to explore the possible occurrence of inducing resistance of schistosoma japonicum to praziquantel (pzq), a set of animal experiments were carried out. outbred mice (nih strain), anhui isolates of s. japonicum and oncomelania hupensis were used. in one protocol five weeks after being infected with 48-52 cercariae, mice were orally dosed with pzq 300 mg/kg, and killed 82 days later to isolate eggs from the liver. snails were exposed to miracidia released from egg-hatching. f1 progeny wer ... | 1990 | 2402679 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. i. compatibility between schistosomes and their snail hosts]. | oncomelania hupensis hupensis from six localities were used in this study, i.e. guichi of anhui in the east, jianli of hubei in the middle, guiping of guangxi in the south, tianquan of sichuan and eryuan of yunnan in the southwest and fuqing of fujian in the southeast. snails from each locality were individually cross-exposed to 20 miracidia of the different isolates of s. japonicum from the above-named places, with the exception of fujian province where no snail could be found naturally infecte ... | 1990 | 2208632 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. ii. susceptibility of mammalian hosts]. | six species of animals were percutaneously exposed to cercariae obtained from pools of naturally infected snails from different isolates of s. japonicum in the mainland of china as shown in tables 1 and 2. our results indicated that with the exception of rat, all animals under study were permissive host though their worm recovery rates varied with different isolates. the mean prepatent period in different host species were 35.0 +/- 0.8 to 36.4 +/- 1.0 days for anhui isolate; 34.5 +/- 1.2 to 36.4 ... | 1990 | 2099256 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. iv. fuzzy cluster analysis on some biological characters of different isolates of schistosoma japonicum]. | in this paper, a computerized numerical systematics was used to analyse the 5 different isolates of schistosoma japonicum in the mainland of china by means of fuzzy clustering method with 32 quantitative data of each isolate about the morphological and biological characters. a dendrogram of the 5 isolates of s. japonicum was constructed on the basis of similarity coefficients as shown. the phylogenetic relationships revealed by the present study show that the two isolates of anhui and hubei get ... | 1991 | 1959166 |
| compatibility between oncomelania hupensis and different isolates of schistosoma japonicum in china. | oncomelania hupensis from six localities were used for infection with different isolates of schistosoma japonicum in the mainland of china, ie anhui in the east, hubei in the center, guangxi in the south, sichuan in the west, yunnan in the southwest and fujian in the southeast. snails from anhui and hubei were readily infected with the local isolate of s. japonicum and cross infection also took place readily between the snails and the schistosomes from these two places. snails from sichuan and y ... | 1991 | 1948286 |
| [studies on strain differences of schistosoma japonicum in the mainland of china. vi. analysis with multilocus enzyme electrophoresis]. | five different field-collected isolates (i.e., anhui, hubei, guangxi, sichuan and yunnan) of schistosoma japonicum from the mainland of china were compared by means of multilocus enzyme electrophoresis in polyacrylamide gels of individual male worm extracts. the enzyme systems used in the study included gdh, g6pd, ldh, mdh, pgi, pgm and sod. of 9 loci examined in the 7 isozyme systems, 4 were found to be polymorphic. these were ldh-1, ldh-2, mdh and pgm, the remainder of gdh, g6pd-1, g6pd-2, pgi ... | 1991 | 1802457 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. vii. genetic variation and differentiation of five isolates]. | electrophoretic techniques have been used to survey five different field-collected isolates (i.e., anhui, hubei, guangxi, sichuan and yunnan) of s. japonicum from the mainland of china for genetic variation at 7 isozymes markers representing 9 loci. polymorphic loci were observed for ldh-1, ldh-2, mdh and pgm, respectively, the proportion of polymorphic loci being 44.4%. average heterozygosity varied between 0.223 and 0.425, with a mean of 0.332. nei's genetic distance (d) among the populations ... | 1992 | 1394885 |
| liver ultrasound findings in a low prevalence area of s. japonicum in china: comparison with history, physical examination, parasitological and serological results. | the paper describes a study carried out in a community in dongdian township, anhui province, people's republic of china. medical history and the results of a physical examination, ultrasound investigation, parasitological and serological tests for schistosoma japonicum infection were compared in 661 persons of 169 households. a lack of correlation between parasitological and serological indicators of infection and morbidity was observed in this area of low (6.4%) prevalence and intensity of infe ... | 1992 | 1351356 |
| [studies on the strain differences of schistosoma japonicum in the mainland of china. x. comparison of sds-page protein patterns]. | the purpose of the present study was to investigate the sds-page separation of proteins of the 5 different isolates of schistosoma japonicum from the mainland of china to determine their similarities and/or differences and to gain additional data which could give an added insight into the degree of relationship. soluble proteins of freshly sonicated adult worm extract of the 5 different isolates (i.e., anhui, hubei, guangxi, sichuan and yunnan) were compared by sds-polyacrylamide gel electrophor ... | 1992 | 1307276 |
| treatment and reinfection of water buffaloes and cattle infected with schistosoma japonicum in yangtze river valley, anhui province, china. | the aim of the present study was to evaluate the efficacy of praziquantel treatment of schistosoma japonicum infections in cattle and water buffaloes and to assess the natural rate of reinfection after treatment. the studies were conducted on 2 islands in the yangtze river, anhui province, china, from march 2003 to january 2004. the efficacy of praziquantel was 97% when applied orally wrapped in tree leaves at the recommended doses. the efficacy was measured using a miracidium hatching technique ... | 2006 | 17152955 |
| does multiple hosts mean multiple parasites? population genetic structure of schistosoma japonicum between definitive host species. | multi-host parasites, those capable of infecting more than one species of host, are responsible for the majority of all zoonotic, emerging or persistent human and animal diseases and are considered one of the major challenges for the biomedical sciences in the 21st century. we characterized the population structure of the multi-host parasite schistosoma japonicum in relation to its definitive host species by genotyping miracidia collected from humans and domestic animals across five villages aro ... | 2006 | 16876170 |
| surveillance and control of post-transmission schistosomiasis in jiaxing prefecture, zhejiang province, china. | a surveillance system has been put in place in the zhejiang province since achieving there the criteria for transmission interruption of schistosomiasis japonica. suspected patients and special high-risk groups (e.g. the so called 'floating population' and children under 14 years of age) are screened for schistosoma japonicum using serological tests. those with positive serological result are subjected to faecal examination and if s. japonicum eggs are found they are treated with praziquantel an ... | 2005 | 16198300 |
| field evaluation of a rapid, visually-read colloidal dye immunofiltration assay for schistosoma japonicum for screening in areas of low transmission. | to determine the validity of a recently developed rapid test--a colloidal dye immunofiltration assay (cdifa)--used by health workers in field settings to identify villagers infected with schistosoma japonicum. | 2005 | 16175827 |
| an economic evaluation of the national schistosomiasis control programme in china from 1992 to 2000. | the world bank loan project, by far the largest effort in china for schistosomiasis control since control activities were initiated in the mid 1950s, was carried out for a 9-year period commencing in 1992 in the 8 provinces where schistosoma japonicum remained endemic when the project started. to evaluate its impact, a retrospective economic evaluation was done in 2001. six representative counties, i.e. huarong in hunan province, qianjiang in hubei province, yugan in jiangxi province, tongling i ... | 2005 | 16154104 |
| an insight into the genetic variation of schistosoma japonicum in mainland china using dna microsatellite markers. | this study presents the first microsatellite investigation into the level of genetic variation among schistosoma japonicum from different geographical origins. s. japonicum isolates were obtained from seven endemic provinces across mainland china: zhejiang (jiashan county), anhui (guichi county), jiangxi (yongxiu county), hubei (wuhan county), hunan (yueyang area), sichuan 1 (maoshan county), sichuan 2 (tianquan county), yunnan (dali county), and also one province in the philippines (sorsogon). ... | 2005 | 15723675 |
| relationship between the transmission of schistosomiasis japonica and the construction of the three gorge reservoir. | to study the relationship between the changes of environment and the transmission of schistosomiasis japonica after the construction of the three gorge reservoir. | 2002 | 12020887 |
| [study on unsupervised classification in marshland of endemic area of schistosomiasis japonica using satellite tm image data]. | to categorize the vegetation of marshland in the endemic areas of schistosomiasis japonica using satellite thematic mapper (tm)data in liupo village, anhui province. | 2000 | 11372390 |
| epidemiology of schistosomiasis in the people's republic of china, 2004. | results from the third nationwide cluster sampling survey on the epidemiology of schistosomiasis in the people's republic of china, conducted by the ministry of health in 2004, are presented. a stratified cluster random sampling technique was used, and 239 villages were selected in 7 provinces where schistosoma japonicum remains endemic. a total of 250,987 residents 6-65 years of age were included in the survey. estimated prevalence rates in the provinces of hunan, hubei, jiangxi, anhui, yunnan, ... | 2007 | 18257989 |
| variation in the sequence of a mitochondrial nadh dehydrogenase i gene fragment among six natural populations of schistosoma japonicum from china. | the present study investigated the level of genetic variation among schistosoma japonicum populations of different geographical origins from mainland china. polymerase chain reaction-based methods were employed to determine the sequence for a subunit of the mitochondrial nadh dehydrogenase i gene for populations from zhejiang, anhui, jiangxi, hunan, hubei and sichuan. no variation was detectable in the nadh dehydrogenase i sequence within populations from zhejiang and hubei, whereas sequence var ... | 1998 | 9925274 |
| genetic variability among schistosoma japonicum isolates from different endemic regions in china revealed by sequences of three mitochondrial dna genes. | the present study examined sequence variation in three mitochondrial dna (mtdna) regions, namely cytochrome c oxidase subunit 3 (cox3), nadh dehydrogenase subunits 4 and 5 (nad4 and nad5), among schistosoma japonicum isolates from different endemic regions in china, and their phylogenetic relationships were re-constructed. a portion of the cox3 gene (pcox3), a portion of the nad4 and nad5 genes (pnad4 and pnad5) were amplified separately from individual trematodes by polymerase chain reaction (p ... | 2009 | 19303214 |
| a biological and immunological comparison of chinese and philippine schistosoma japonicum. | a comparison was made of the host (balb/c mice)-parasite relationship of a chinese mainland (anhui) strain and philippine (sorsogon) strain of schistosoma japonicum. differences in fecundity, infectivity, prepatency, host survival and susceptibility to praziquantel were examined. the mean percentage of worms establishing was 40.0 and 34.4% for chinese and philippine s. japonicum, respectively. egg laying commenced on days 26 and 28 after infection with the chinese or philippine strain, respectiv ... | 1996 | 8786224 |
| parasite genetic differentiation by habitat type and host species: molecular epidemiology of schistosoma japonicum in hilly and marshland areas of anhui province, china. | schistosoma japonicum, a parasite of significant public health importance in parts of china and southeast asia, is a true generalist pathogen with over 40 species of mammals suspected as definitive host reservoirs. in order to characterize levels of parasite gene flow across host species and identify the most important zoonotic reservoirs, s. japonicum larvae (miracidia) were sampled from a range of definitive host species in two contrasting habitat types within anhui province, china: a low-lyin ... | 2009 | 19389178 |
| landscape genetics: the correlation of spatial and genetic distances of oncomelania hupensis, the intermediate host snail of schistosoma japonicum in mainland china. | two internal transcribed spacer (its1 and its2) sequences of the non-coding region of the rdna and a 16s rna gene fragment of the mtdna of oncomelania hupensis, the intermediate host snail of schistosoma japonicum in mainland china, have been investigated with a view to illustrate the influence of the environment on genetic differentiation. thirteen populations of o. hupensis snails, representing four types of ecological settings, were collected to compare genetic and spatial distances. the leng ... | 2009 | 19440964 |
| issr, an effective molecular approach for studying genetic variability among schistosoma japonicum isolates from different provinces in mainland china. | in the present study, inter-simple sequence repeats (issrs) markers were used to examine the genetic variability of schistosoma japonicum isolates from different provinces in mainland china, using s. japonicum from japan and s. mansoni from puerto rico for comparison. of the 30 primers screened, 4 produced highly reproducible issr fragments. using these primers, 107 discernible dna fragments were generated with 105 (98.13%) being polymorphic, indicating considerable genetic variation among the e ... | 2009 | 19539784 |
| real-time polymerase chain reaction for detection of low-intensity schistosoma japonicum infections in china. | more sensitive methods for diagnosing infection with schistosoma japonicum are needed as control becomes more effective. we compared a real-time polymerase chain reaction (pcr) for stool samples with conventional diagnostic methods in a study of 1,727 persons from anhui province, china. seroprevalence determined by using an indirect hemagglutination assay (iha) was much higher (26.1%) than the prevalence in stool-based tests, which were 5.3%, 3.2%, and 3.0% for pcr, hatching test, and kato-katz ... | 2009 | 19706908 |
| contrasting reservoirs for schistosoma japonicum between marshland and hilly regions in anhui, china--a two-year longitudinal parasitological survey. | schistosoma japonicum remains highly endemic in many counties in china and has recently re-emerged, to a large extent, in previously controlled areas. to test the hypothesis that small rodents and less agriculturally important domestic animals such as dogs and cats may play an important role in the transmission and potential re-emergence of this disease, an annual investigation of s. japonicum among humans, domestic animals and rodents, combined with detailed surveys of the snail intermediate ho ... | 2010 | 19723358 |
| china's new strategy to block schistosoma japonicum transmission: experiences and impact beyond schistosomiasis. | despite sustained efforts for its control made over the past 50+ years, the re-emergence of schistosomiasis in china was noted around the turn of the new millennium. consequently, a new integrated strategy was proposed to stop the contamination of schistosome eggs to the environment, which emphasizes health education, access to clean water and adequate sanitation, mechanization of agriculture and fencing of water buffaloes, along with chemotherapy. validation of this integrated control strategy ... | 2009 | 19793080 |
| mathematical modelling and control of schistosomiasis in hubei province, china. | hubei province, along with four other provinces in the central and eastern china where schistosomiasis is endemic (anhui, hunan, jiangsu, and jiangxi), is located in the lake and marshland regions along the yangtze river. high population density, large numbers of farm cattle, and huge areas of snail habitat are the main characteristics that maintain the persistence of the disease and the transmission of the parasite schistosoma japonicum in these regions. based on the schistosomiasis infection d ... | 2010 | 20188689 |
| distinct genetic diversity of oncomelania hupensis, intermediate host of schistosoma japonicum in mainland china as revealed by its sequences. | oncomelania hupensis is the unique intermediate host of schistosoma japonicum, which causes schistosomiasis endemic in the far east, and especially in mainland china. o. hupensis largely determines the parasite's geographical range. how o. hupensis's genetic diversity is distributed geographically in mainland china has never been well examined with dna sequence data. | 2010 | 20209150 |
| transmission of schistosoma japonicum in marshland and hilly regions of china: parasite population genetic and sibship structure. | the transmission dynamics of schistosoma japonicum remain poorly understood, as over forty species of mammals are suspected of serving as reservoir hosts. however, knowledge of the population genetic structure and of the full-sibship structuring of parasites at two larval stages will be useful in defining and tracking the transmission pattern between intermediate and definitive hosts. s. japonicum larvae were therefore collected in three marshland and three hilly villages in anhui province of ch ... | 2010 | 20689829 |
| an integrated strategy for transmission control of schistosoma japonicum in a marshland area of china: findings from a five-year longitudinal survey and mathematical modeling. | in 2004 an aggressive plan was instituted aiming to achieve nationwide transmission control of schistosomiasis by 2015. here, we report a longitudinal study on the control of schistosomiasis in anhui province, china. using a mathematical model, we compared the effects of different control strategies implemented in the study area. during the 5-year study period, a 60.8% reduction in human prevalence was observed from 2005 (7.95%) to 2009 (3.1%), and snail infection decreased from 0.063% in 2005 t ... | 2011 | 21734130 |
| compatibility of schistosoma japonicum from the hilly region and oncomelania hupensis hupensis from the marshland region within anhui, china. | schistosome japonicum remains one main public concern in china. this is exemplified in the hilly region in anhui province, where rodents have served as reservoirs for the parasite and no effective intervention could target such wild animals. the closer relationship between the hilly region and the near marshland induces the worry of spread of the hill parasite to the marshland region. therefore, the level of snail-parasite compatibility between the hill parasite and snail populations from the ya ... | 2014 | 25199558 |
| new integrated strategy emphasizing infection source control to curb schistosomiasis japonica in a marshland area of hubei province, china: findings from an eight-year longitudinal survey. | schistosomiasis remains a major public health problem in china. the major endemic foci are the lake and marshland regions of southern china, particularly the regions along the middle and lower reach of the yangtze river in four provinces (hubei, hunan, jiangxi, and anhui). the purpose of our study is to assess the effect of a new integrated strategy emphasizing infection source control to curb schistosomiasis in marshland regions. | 2014 | 24587030 |
| monitoring the transmission of schistosoma japonicum in potential risk regions of china, 2008 - 2012. | schistosomiasis japonica, caused by schistosoma japonicum infection, remains a major public health concern in china, and the geographical distribution of this neglected tropical disease is limited to regions where oncomelania hupensis, the intermediate host of the causative parasite, is detected. the purpose of this study was to monitor the transmission of s. japonicum in potential risk regions of china during the period from 2008 through 2012. to monitor the transmission, 10 fixed surveillance ... | 2014 | 24566053 |
| single- or mixed-sex schistosoma japonicum infections of intermediate host snails in hilly areas of anhui, china. | schistosomiasis japonicum is one of the most serious communicable diseases, and the transmission of the parasite is dependent of its complex life cycle on which many factors can have an impact. multiple infections comprising both male and female schistosome within snail intermediate hosts, for example, would facilitate parasite transmission. however, no research on schistosoma japonicum communities in field-collected oncomelania hupensis hupensis in relation to schistosome sex has been reported. ... | 2014 | 24292605 |
| a circular analysis of chronobiology of schistosoma japonicum cercarial emergence from hilly areas of anhui, china. | about 46 mammal species have been suspected as reservoir hosts for schistosoma japonicum and therefore the track of the target parasites, in relation to definitive host species, may be of great importance in terms of theoretical and practical implications. the circadian rhythm of cercariae emergence, a genetically controlled behavior for parasites to adapt to their definitive hosts, may seem to be a perfect biological marker for s. japonicum. in this study, a late (or nocturnal) cercarial emerge ... | 2013 | 23988664 |
| retrotransposon-microsatellite amplified polymorphism, an electrophoretic approach for studying genetic variability among schistosoma japonicum geographical isolates. | in the present study, retrotransposon-microsatellite amplified polymorphism (remap) was used to examine genetic variability among schistosoma japonicum isolates from different endemic provinces in mainland china, using s. japonicum from japan and the philippines for comparison. of the 50 primer combinations screened, eight produced highly reproducible remap fragments. using these primers, 190 distinct dna fragments were generated in total, of which 147 (77.37%) were polymorphic, indicating consi ... | 2012 | 23019103 |
| a specific pcr assay for the identification and differentiation of schistosoma japonicum geographical isolates in mainland china based on analysis of mitochondrial genome sequences. | in the present study, near-complete mt genome sequences for eight representative schistosoma japonicum samples from seven endemic provinces in mainland china were analyzed. sequence differences among the eight mt genomes of s. japonicum samples were 0.20-2.51%. variation in protein-coding genes was greater than that in rrna genes. the mt dna sequences of s. japonicum samples from south-western (sw) china were 2 bp [position 11727-11728 within trna-cys, microsatellite (ag) indel] longer than thos ... | 2012 | 22446475 |
| genetic diversity and structure of schistosoma japonicum within two marshland villages of anhui, china, prior to schistosome transmission control and elimination. | schistosomiasis is caused by the genus schistosoma and affected more than 250 million people worldwide. schistosoma japonicum was once seriously endemic in china and nearly 60 years of efforts has seen great success in disease control. however, due to its zoonotic nature and complex life cycle, the schistosomiasis transmission control and final elimination would require, besides an intersectoral approach, deep understanding of population genetics of the parasite. we therefore performed a snail s ... | 2017 | 27838835 |
| genetic variation between schistosoma japonicum lineages from lake and mountainous regions in china revealed by resequencing whole genomes. | schistosoma infection is a major cause of morbidity and mortality worldwide. schistosomiasis japonica is endemic in mainland china along the yangtze river, typically distributed in two geographical categories of lake and mountainous regions. study on schistosome genetic diversity is of interest in respect of understanding parasite biology and transmission, and formulating control strategy. certain genetic variations may be associated with adaptations to different ecological habitats. the aim of ... | 2016 | 27207135 |
| shifts in the spatiotemporal dynamics of schistosomiasis: a case study in anhui province, china. | the chinese national surveillance system showed that the risk of schistosoma japonicum infection fluctuated temporally. this dynamical change might indicate periodicity of the disease, and its understanding could significantly improve targeted interventions to reduce the burden of schistosomiasis. the goal of this study was to investigate how the schistosomiasis risk varied temporally and spatially in recent years. | 2015 | 25881189 |