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oral colonisation by aerobic and facultatively anaerobic gram-negative rods and yeast in tibetans living in lhasa.sample groups of children (n=50) and adults (n=38) were selected from pools of 207 children, (11-13-year olds from two primary schools) and 94 adults (25-44-year olds from four governmental agencies) who were the subjects of an oral health survey among tibetans living in lhasa, tibet autonomous region. mean ages of the study groups of children (38% females) and adults (61% females) were 11.6+/-0.9 and 37.1+/-6.1 years, respectively. all had lived in tibet since birth. oral rinse samples were sel ...200312642230
two new antifungal saponins from the tibetan herbal medicine clematis tangutica.bioassay-guided fractionation of the ethanol extract of the aerial parts of clematis tangutica led to the isolation of two new antifungal triterpene saponins. their structures were determined to be 3- o-alpha- l-arabinopyranosyl hederagenin 28- o-alpha- l-rhamnopyranosyl ester ( 1) and 3- o-beta- d-glucopyranosyl-(1--> 4)-alpha- l-arabinopyranosyl hederagenin 28- o-alpha- l-rhamnopyranosyl ester ( 2) on the basis of spectral data and chemical evidence. inhibitory activities of the two saponins a ...200312865975
candida tibetensis sp. nov. and candida linzhiensis sp. nov., novel anamorphic, ascomycetous yeast species from tibet.three anamorphic, ascomycetous yeast strains isolated from plant samples collected in linzhi district, tibet, china, were revealed as representing two novel species by 26s rrna gene d1/d2 domain sequence and physiological property comparisons. the names candida tibetensis sp. nov. and candida linzhiensis sp. nov. are proposed for these novel species, with xz 41-6t (=as 2.3072t=cbs 10298t) and xz 92-1t (=as 2.3073t=cbs 10299t) as the respective type strains. d1/d2 sequence analysis showed that c. ...200616627670
chemical composition, antimicrobial and antioxidant activities of the essential oil of tibetan herbal medicine dracocephalum heterophyllum benth.the essential oil of tibetan medicine dracocephalum heterophyllum benth was obtained by hydrodistillation with a 0.7% (v/w) yield. the chemical composition of the essential oil was analyzed by gas chromatography-mass spectral (gc-ms). eighty-three compounds, constituting about 89.83% of the total oil, were identified. the main compound in the oil were cineole (14.89%), trans-nerolido (7.10%), 1-m-ethyl-2-(1-methylethyl)-benzene (4.42%), germacrene-d (4.84%), decahydro-1,1,4,7-tetramethyl-4ah-cyc ...200817999332
comparison of nutrition and microbiological compositions between two types of fermented milk from tibet in china.twenty-nine traditional fermented yak milk (kurut) and 15 traditional fermented mongolian cow milk (fmcm) samples were collected from three regions of tibet in china, and their chemical composition and microbiological properties were analyzed. average levels of the major composition of kurut and fmcm, respectively, were as follows: total solid, 16.91+/-1.30 g/100 g and 12.65+/-1.14 g/100 g; fat, 6.83+/-1.18 g/100 g and 3.92+/-0.92 g/100 g; total protein, 4.93+/-0.36 g/100 g and 3.33+/-0.71 g/100 ...200919521897
rhizobium tibeticum sp. nov., a symbiotic bacterium isolated from trigonella archiducis-nicolai (sirj.) vassilcz.isolated from root nodules of trigonella archiducis-nicolai (sirj.) vassilcz. grown in tibet, china, cells of the bacterial strains ccbau 85039(t) and ccbau 85027 were gram-negative, aerobic, motile, non-spore-forming rods that formed colonies that were semi-translucent and opalescent on yeast extract-mannitol agar. in numerical taxonomy, sds-page analysis of whole-cell proteins and dna-dna hybridization, the two strains were very similar and were different from reference strains of defined rhiz ...200919643889
torulaspora quercuum sp. nov. and candida pseudohumilis sp. nov., novel yeasts from human and forest habitats.strains xz-46a, xz-105, xz-129 and xz-281(t) isolated from the oral cavities of healthy tibetan volunteers were revealed to represent two novel ascomycetous yeast species by molecular taxonomic characterizations. strain xz-281(t) was most closely related to candida humilis, but differed from the type strain of the species by eight (1.2%) substitutions in the 26s rrna gene d1/d2 domain and by >100 (>20%) mismatches in the internal transcribed spacer (its) region. strains xz-46a, xz-105 and xz-129 ...200919751217
analysis of the microflora in tibetan kefir grains using denaturing gradient gel electrophoresis.the microflora of tibetan kefir grains was investigated by culture- independent methods. denaturing gradient gel electrophoresis (dgge) of partially amplified 16s rrna for bacteria and 26s rrna for yeasts, followed by sequencing of the most intense bands, showed that the dominant microorganisms were pseudomonas sp., leuconostoc mesenteroides, lactobacillus helveticus, lactobacillus kefiranofaciens, lactococcus lactis, lactobacillus kefiri, lactobacillus casei, kazachstania unispora, kluyveromyce ...200919835760
occurrence and dominance of yeast species in naturally fermented milk from the tibetan plateau of china.to determine which yeasts are present in the naturally fermented milks of china, 69 samples made by the nomads of tibet were collected from the tibetan plateau in china. from these samples, 225 strains of yeast were isolated and identified using conventional microbiological analysis and gene sequencing analysis of the d1/d2 domain of the large subunit (26s) ribosomal dna. the results showed that the total concentration of yeasts in these samples ranged from 5.01 to 8.97 log10 colony-forming unit ...201020921981
[identification and biodiversity of yeasts from qula in tibet and milk cake in yunnan of china].to analyze the biodiversity and distribution of the yeast species of qula in tibet and milk cake in yunnan, and to provide essential data for the utilization of yeasts in the traditional dairy products of china.201021090253
bensingtonia rectispora sp. nov. and bensingtonia bomiensis sp. nov., novel ballistoconidium-forming yeast species from plant leaves collected in tibet.five yeast strains isolated from plant leaves collected in southeast tibet formed cream to brownish colonies and produced asymmetrical ballistoconidia and coq-9 as the major ubiquinone. sequence analysis of the 26s rrna d1/d2 domain and the internal transcribed spacer (its) region indicated that these strains represented two novel species of the genus bensingtonia. bensingtonia rectispora sp. nov. (type strain: xz 4c5t = cgmcc 2.02635t = cbs 10710 t) and bensingtonia bomiensis sp. nov. (type str ...201122081720
investigation on culturable microflora in tibetan kefir grains from different areas of china.four samples of tibetan kefir grains (tk-zjuj 01-04) from tibet and surrounding areas were investigated via phenotypic and genotypic methods to compare and analyze the diversity of culturable microflora among different origins. as a result, 4 genera of microorganisms from tk-zjuj01: bacillus subtilis (2.9 × 10(7) cfu/ml), lactococcus lactis (8.2 × 10(7) cfu/ml), kluyveromyces marxianus (3.0 × 10(6) cfu/ml), saccharomyces cerevisiae (9.0 × 10(6) cfu/ml); 4 genera from tk-zjuj02: lactobacillus kef ...201222860591
fine structure of tibetan kefir grains and their yeast distribution, diversity, and shift.tibetan kefir grains (tkgs), a kind of natural starter for fermented milk in tibet, china, host various microorganisms of lactic acid bacteria, yeasts, and occasionally acetic acid bacteria in a polysaccharide/protein matrix. in the present study, the fine structure of tkgs was studied to shed light on this unusual symbiosis with stereomicroscopy and thin sections. the results reveal that tkgs consist of numerous small grain units, which are characterized by a hollow globular structure with a di ...201424977409
complex ancestries of lager-brewing hybrids were shaped by standing variation in the wild yeast saccharomyces eubayanus.lager-style beers constitute the vast majority of the beer market, and yet, the genetic origin of the yeast strains that brew them has been shrouded in mystery and controversy. unlike ale-style beers, which are generally brewed with saccharomyces cerevisiae, lagers are brewed at colder temperatures with allopolyploid hybrids of saccharomyces eubayanus x s. cerevisiae. since the discovery of s. eubayanus in 2011, additional strains have been isolated from south america, north america, australasia ...201627385107
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