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development of real-time polymerase chain reaction for detection of borrelia burgdorferi sensu lato in china.universal primers and probes were selected on the basis of the 16s rrna gene sequence of borrelia burgdorferi in genbank®, and a real-time polymerase chain reaction (pcr) method for detection of b. burgdorferi was established. the results showed that this method could specifically detect the b31 strain (borrelia burgdorferi sensu stricto), the bo23 strain (borrelia afzelii), and the sz strain (borrelia garinii), without cross-reaction with genome dna of theileria (t. luwenshuni, t. uilenbergi, t ...201222448677
[isolation and identification of borrelia burgdorferi sensu lato from ticks in six provinces in china.]objective: to understand the carrying status of borrelia burgdorferi in ticks from the mountain areas from six representative provinces, including jilin, shanxi, gansu, qinghai, guizhou and hunan in china. methods: flagging and trapping methods were used to collect ticks in forest area and culture medium was used to isolate the pathogen. nested-pcr was used to detect the germ-carrying rate of ticks. results: more than 2200 ticks from six representative provinces were collected and 1000 ticks wer ...201021223661
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