Publications

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environmental investigations of vibrio parahaemolyticus in oysters after outbreaks in washington, texas, and new york (1997 and 1998).total vibrio parahaemolyticus densities and the occurrence of pathogenic strains in shellfish were determined following outbreaks in washington, texas, and new york. recently developed nonradioactive dna probes were utilized for the first time for direct enumeration of v. parahaemolyticus in environmental shellfish samples. v. parahaemolyticus was prevalent in oysters from puget sound, wash.; galveston bay, tex.; and long island sound, n.y., in the weeks following shellfish-associated outbreaks ...200011055906
incidence of vibrio parahaemolyticus in u.s. coastal waters and oysters.oyster and seawater samples were collected seasonally from may 1984 through april 1985 from shellfish-growing areas in washington, california, texas, louisiana, alabama, florida, south carolina, virginia, and rhode island which had been designated as approved or prohibited by the national shellfish sanitation program. fecal coliforms counts, aerobic plate counts, and vibrio parahaemolyticus densities were determined for the samples. mean v. parahaemolyticus density was more than 100 times greate ...19902403249
automated ribotyping differentiates vibrio parahaemolyticus o3:k6 strains associated with a texas outbreak from other clinical strains.automated ribotyping with a qualicon riboprinter was used to determine whether clinical isolates of vibrio parahaemolyticus o3:k6 recovered during two u.s. outbreaks of oyster-associated gastroenteritis in 1998 were related to each other and to a previously identified highly virulent asian clone of this serotype. the patterns produced using the restriction enzymes eco ri and pst i suggest that the outbreak in the northeastern united states was caused by a single strain closely related to the asi ...200111601716
characterization of united states outbreak isolates of vibrio parahaemolyticus using enterobacterial repetitive intergenic consensus (eric) pcr and development of a rapid pcr method for detection of o3:k6 isolates.outbreaks of vibrio parahaemolyticus gastroenteritis in the united states (texas, new york and pacific northwest) in 1997-98 emphasized the need to develop molecular methods for identification and differentiation of these organisms. when outbreak isolates were analyzed for the enterobacterial repetitive intergenic consensus sequences, the texas and new york outbreak isolates had a specific 850-bp dna fragment that was absent in pacific northwest isolates. the 850-bp polymerase chain reaction (pc ...200211814665
molecular, serological, and virulence characteristics of vibrio parahaemolyticus isolated from environmental, food, and clinical sources in north america and asia.potential virulence attributes, serotypes, and ribotypes were determined for 178 pathogenic vibrio parahaemolyticus isolates from clinical, environmental, and food sources on the pacific, atlantic, and gulf coasts of the united states and from clinical sources in asia. the food and environmental isolates were generally from oysters, and they were defined as being pathogenic by using dna probes to detect the presence of the thermostable direct hemolysin (tdh) gene. the clinical isolates from the ...200312839774
incidence of vibrio species associated with blue crabs (callinectes sapidus) collected from galveston bay, texas.bacteria were readily isolated from the hemolymph of a majority (88%) of the blue crabs collected from galveston bay, texas. the hemolymph of most crabs contained moderate (greater than 10(3) bacteria/ml) to heavy (greater than (10(5) bacteria/ml) infections. large variances were observed in the bacterial number associated with individual crabs, but no significant difference was observed between the mean bacterial levels in the hemolymph of crabs collected during different seasons of the samplin ...19827103475
occurrence of vibrio vulnificus and toxigenic vibrio parahaemolyticus on sea catfishes from galveston bay, texas.dorsal and pectoral fin spines from two species of sea catfishes (bagre marinus and ariopsis felis) landed at 54 sites in galveston bay, texas, and its sub-bays from june to october 2005 were screened with traditional cultivation-based assays and quantitative pcr assays for vibrio vulnificus and vibrio parahaemolyticus. v. vulnificus was present on 51.2% of fish (n = 247), with an average of 403 ± 337 sd cells g(-1). v. parahaemolyticus was present on 94.2% (n = 247); 12.8% tested positive for t ...201425285498
emergence of a new vibrio parahaemolyticus serotype in raw oysters: a prevention quandary.in may and june 1998, reported vibrio parahaemolyticus infections increased sharply in texas.200011000648
crayfish: a newly recognized vehicle for vibrio infections.we conducted a 1-year case-control study of sporadic vibrio infections to identify risk factors related to consumption of seafood products in two coastal areas of louisiana and texas. twenty-six persons with sporadic vibrio infections and 77 matched controls were enrolled. multivariate analysis revealed that crayfish (p < 0.025) and raw oysters (p < 0.009) were independently associated with illness. species-specific analysis revealed an association between consumption of cooked crayfish and vibr ...19989825776
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