| prevalence of giardia duodenalis genotypes in pre-weaned dairy calves. | to determine the prevalence of giardia genotypes in pre-weaned dairy calves, fecal samples were collected from a minimum of 18, 1-7-week-old dairy calves per farm on two farms each in the states of vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. samples cleaned of fecal debris and concentrated using cscl density gradient centrifugation were stained and examined by immunofluorescence microscopy and also subjected to pcr and gene sequence analysis. prevalence by p ... | 2004 | 15381298 |
| prevalence and genotypes of giardia duodenalis in post-weaned dairy calves. | to determine the prevalence of giardia genotypes in post-weaned dairy calves, fecal specimens were collected from 3 to 11-month-old dairy calves per farm on two farms in vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. specimens cleaned of fecal debris and concentrated using cscl density gradient centrifugation were stained and examined by immunofluorescence microscopy and also subjected to pcr and dna sequence analysis. overall, pcr provided more sensitive detec ... | 2005 | 15925721 |
| prevalence and genotypes of giardia duodenalis in 1-2 year old dairy cattle. | to determine the prevalence of giardia genotypes in 12-24 month old dairy heifers, fecal specimens were collected from two farms each in vermont, new york, pennsylvania, maryland, virginia, north carolina, and florida. specimens, cleaned of fecal debris and concentrated using cscl density gradient centrifugation, were subjected to pcr and dna sequence analysis. prevalence of giardia infection, ranged from 11% to 75% on 14 farms with an average prevalence of 36% (204 positive cattle out of 571 ex ... | 2006 | 16647818 |
| enterocytozoon bieneusi, giardia, and cryptosporidium infecting white-tailed deer. | despite a white-tailed deer (wtd) population in the united states of approximately 32 million animals extremely little is known of the prevalence and species of the protists that infect these animals. this study was undertaken to determine the presence of potential human protist pathogens in culled wtd in central maryland. feces from fawns to adults were examined by molecular methods. the prevalence of enterocytozoon bieneusi, cryptosporidium, and giardia was determined by pcr. all pcr-positive ... | 2015 | 25066778 |
| a longitudinal study of giardia duodenalis genotypes in dairy cows from birth to 2 years of age. | fecal specimens were collected from 30 calves from birth to 24 months of age at a dairy farm in maryland to determine the prevalence of giardia duodenalis genotypes in cattle of different ages. fecal samples were subjected to density gradient centrifugation to remove debris and concentrate cysts. specimens were analyzed by immunofluorescence microscopy and polymerase chain reaction (pcr). all pcr positive specimens were sequenced using the ssu-rrna gene of giardia. all 30 calves shed g. duodenal ... | 2009 | 19264407 |
| detection of assemblage a, giardia duodenalis and eimeria spp. in alpacas on two maryland farms. | sixty-one fecal samples were collected from adult alpacas and crias (ages 10 weeks to 10 years) on two farms in central maryland. the farms raised both suri (silky-haired) and huacaya (crimpy-haired) breeds. females and crias were housed together on pasture, whereas older/breeding males were maintained on separate pastures. samples were subjected to a density gradient centrifugation protocol to concentrate parasites and remove fecal debris and were examined by immuno-fluorescent and differential ... | 2008 | 18387745 |
| prevalence of cryptosporidium, giardia and eimeria infections in post-weaned and adult cattle on three maryland farms. | the prevalence of cryptosporidium, giardia and eimeria, in healthy, asymptomatic, post-weaned and mature cattle was investigated on three maryland farms. one farm, a dairy research facility, had 150 multiparous holstein milking cows; 24 were examined and cryptosporidium andersoni was detected in three (12.5%) but neither giardia nor eimeria was detected. the second farm, a commercial dairy, had 57 multiparous holstein milking cows and an equal number of heifers. of 19 cows examined, c. parvum, g ... | 2000 | 11035228 |
| giardia duodenalis cysts of genotype a recovered from clams in the chesapeake bay subestuary, rhode river. | filter-feeding molluscan shellfish can concentrate zoonotic and anthroponotic waterborne pathogens. cysts of giardia sp. were detected by immunofluorescent antibodies in tissues of the clams macoma balthica and m. mitchelli from rhode river, a chesapeake bay (maryland) subestuary. molecular tests identified the cysts as giardia duodenalis genotype a, the most common genotype recovered from humans. macoma clams are burrowers in mud or sandy-mud substrata and preferentially feed on the surface sed ... | 1999 | 10548284 |