| quantitative real-time pcr for detection and identification of candidatus liberibacter species associated with citrus huanglongbing. | citrus huanglongbing (hlb, ex greening) is one of the most serious diseases of citrus. different forms of the disease are caused by different candidatus liberobacter species, candidatus liberibacter asiaticus (las), ca. l. africanus (laf) and ca. l. americanus (lam). the pathogen is transmitted by psyllid insects and by budding with contaminated plant materials. the vector psyllid diaphorina citri can transmit both las and lam. establishment of this vector into florida, reports of lam and las in ... | 2006 | 16414133 |
| incidence of invasive diaphorina citri (hemiptera: psyllidae) and its introduced parasitoid tamarixia radiata (hymenoptera: eulophidae) in florida citrus. | diaphorina citri kuwayama (hemiptera: psyllidae), vectors the bacterium candidatus liberibacter asiaticus, one of the causal organisms of the devastating citrus disease "huanglongbing" or citrus greening. in the united states, d. citri was first discovered in florida, in 1998. tamarixia radiata waterston (hymenoptera: eulophidae) was imported from asia and released in florida in 1999-2001 to improve biological control of d. citri before citrus greening was detected in florida in 2005. florida ci ... | 2009 | 19253643 |
| isolation and characterization of beneficial bacteria associated with citrus roots in florida. | cultivable diversity of bacteria associated with citrus was investigated as part of a larger study to understand the roles of beneficial bacteria and utilize them to increase the productive capacity and sustainability of agro-ecosystems. citrus roots from huanglongbing (hlb) diseased symptomatic and asymptomatic citrus were used in this study. a total of 227 and 125 morphologically distinct colonies were isolated and characterized from hlb asymptomatic and symptomatic trees, respectively. we obs ... | 2011 | 21360139 |
| diversity and plasticity of the intracellular plant pathogen and insect symbiont, 'candidatus liberibacter asiaticus', revealed by hyper variable prophage genes with intragenic tandem repeats. | 'candidatus liberibacter asiaticus' (las) is a psyllid-transmitted, phloem-limited alpha proteobacterium, and the most prevalent species of liberibacter associated with a devastating worldwide citrus disease known as huanglongbing (hlb). two related and hyper variable genes (hyvi and hyvii) were identified in the prophage regions of the psy62 las genome. sequence analyses of the hyvi/hyvii genes in 35 las dna isolates collected globally revealed hyvi gene contains up to12 nearly identical tandem ... | 2011 | 21784907 |
| transcriptional response of susceptible and tolerant citrus to infection with candidatus liberibacter asiaticus. | candidatus liberibacter asiaticus (las), a non-culturable phloem-limited bacterium, is the suspected causal agent of huanglongbing (hlb) in florida. hlb is one of the most devastating diseases of citrus and no resistant cultivars have been identified to date, though tolerance has been observed in the genus poncirus and some of its hybrids. this study compares transcriptional changes in tolerant us-897 (citrus reticulata blanco×poncirus trifoliata l. raf.) and susceptible 'cleopatra' mandarin (c. ... | 2011 | 22325873 |
| detection and characterization of miniature inverted-repeat transposable elements in “candidatus liberibacter asiaticus”. | miniature inverted-repeat transposable elements (mites) are nonautonomous transposons (devoid of the transposase gene tps) that affect gene functions through insertion/deletion events. no transposon has yet been reported to occur in “candidatus liberibacter asiaticus,” an alphaproteobacterium associated with citrus huanglongbing (hlb, yellow shoot disease). in this study, two mites, mclas-a and mclas-b, in “ca. liberibacter asiaticus” were detected, and the genome was characterized using 326 iso ... | 2013 | 23813735 |
| seasonal movement patterns and long-range dispersal of asian citrus psyllid in florida citrus. | the asian citrus psyllid, diaphorina citri kuwayama, is the vector of the bacterial pathogen, candidatus liberibacter asiaticus, which is the causal agent of huanglongbing (hlb) in the united states. both short-range and long-range dispersal of d. citri adults affect the spread of hlb; however, little is known about the long-range dispersal capabilities of d. citri in the field or the seasonality of flight behavior. in the present study, an in situ protein marking technique was used to determine ... | 2015 | 26470097 |