[genetic analysis of west nile fever virus, isolated in the south of the russian plain (volgograd and astrakhan regions) in 1999].two strains of west nile virus, vlg 27889 and ast 986, were isolated from the brain of a dead man and from the blood of a patient, respectively, during an outbreak of serous meningitis and meningoencephalitis in july-september, 1999, in the volgograd and astrakhan regions. analysis of parts of genome of the strains cloned from cell culture by reverse transcription and polymerase chain reaction demonstrated their identity and appurtenance to group i west nile viruses.200111233290
outbreak of west nile virus infection, volgograd region, russia, 1999.from july 25 to october 1, 1999, 826 patients were admitted to volgograd region, russia, hospitals with acute aseptic meningoencephalitis, meningitis, or fever consistent with arboviral infection. of 84 cases of meningoencephalitis, 40 were fatal. fourteen brain specimens were positive in reverse transcriptase-polymerase chain reaction assays, confirming the presence of west nile/kunjin virus.200111266303
[genotyping of west nile fever virus strains circulating in southern russia as an epidemiological investigation method: principles and results].characteristic of west nile fever (wnf) virus strains circulating in southern russia.201121598612
[detection of west nile virus rna and specific antibodies in blood donors of volgograd region].study infection level by west nile virus (wnv) of donors living on the territory of west nile fever (wnf) foci with long-term activity, and evaluate the possibility of non-transmissible transfer of the causative agent during use of donor blood.201526016347
the incidence of west nile disease in russia in relation to climatic and environmental factors.since 1999, human cases of west nile fever/neuroinvasive disease (wnd) have been reported annually in russia. the highest incidence has been recorded in three provinces of southern european russia (volgograd, astrakhan and rostov provinces), yet in 2010-2012 the distribution of human cases expanded northwards considerably. from year to year, the number of wnd cases varied widely, with major wnd outbreaks in 1999, 2007, 2010, and 2012. the present study was aimed at identifying the most important ...201424464233
[the need for human health protection from climate changes].the occurring climate changes have a considerable impact on human health. the higher frequency of different natural disasters, including flood and typhoons, deteriorates the epidemiological situation. evidence is provided for the importance of climate warming as a risk factor for a number of communicable diseases, including west nile fever in the astrakhan and volgograd regions. the paper presents the results of analytical epidemiological surveys, by using the time-series analysis, in tver and m ...200920000092
epidemiology of west nile infection in volgograd, russia, in relation to climate change and mosquito (diptera: culicidae) 1999, there was the large outbreak of west nile fever (wnf) in southern russia (>500 cases in the volgograd province). in 2000-2004, the wnf incidence rate decreased steadily to zero, but a new outbreak occurred in 2007 (64 cases). the analysis of historical climate data for volgograd from 1900 to present showed that the years 1999 and 2007 were the hottest ones due to a very mild "winter" (dec.-mar.) and a hot "summer" (june-sep.). there are up to 15 potential wnf vectors in volgograd, but o ...200819030885
[persistence of antibodies specific to west nile virus in blood of reconvalescents of volgograd region].determine the duration of persistence of igm and igg in reconvalescents of west nile fever (wnf) 1 year after the disease in southern regions of russia and evaluate effectiveness of pcr method for acute infection diagnostics.201525816520
[a complex of blood-sucking mosquitoes (diptera, culicidae) in the focus of west nile fever in the volgograd region. iii. species feeding on birds and man and the rhythms of their nocturnal activity].the rate and nocturnal rhythm of mosquito attacks of birds and human beings were studied in the open biotopes of volgograd and its vicinity in 2004. thirteen and 11 species of the subfamily culicinae were collected under the berezantsev bell and from the traps containing a chicken (a hen), respectively; of them 9 species were common. the mosquitoes of an anopheles maculipennis complex were caught in a small portion to the traps of both types. most species of aedes were highly anthropophilic, sho ...200718277420
[the effects of global climatic changes on bloodsucking ectoparasites and pathogens they transmit].the present global climatic changes, regardless of their causes and duration, are of paramount importance from the ecological perspective. the influence of these changes on russian population health attracts the attention of experts. the most important changes have already taken place in the high and middle latitudes, occupied by russia. the article covers a probable impact of global changes on the distribution of bloodsucking arthropods as the vectors of inoculable disease agents, their abundan ...200616734339
[mosquito complex (diptera, culicidae) in a west nile fever focus in the volgograd region. i. species diversity and relative abundance in different habitats].potential west nile virus (wnv) vectors were assessed during 2003 and 2004 at the indoor and outdoor collection sites of volgograd and surrounding rural areas. a total of 26 387 female mosquitoes comprising 16 species in 5 genera of the subfamily culicinae and 4 species of the subfamily anophelinae were collected. the seasonal abundance and the dominance structure greatly differed in the multi-storied buildings, farms, and open habitats whereas the similarity of species diversity remained high t ...200717436731
[the genotyping of the west nile virus in birds in the far eastern region of russia in 2002-2004].samples from 20 species of trapped and dead birds were collected in the far eastern region in 2002-2004 and were analyzed by the anti-wnv mab-modified immunoenzyme assay for antigen detection and rt-pcr for viral rna detection. five positive samples from cinereous vultures (aegypius monachus) and two positive samples from cattle egret (bubulcus ibis) were found in both tests. the sequencing of the 322 bp fragments of protein e gene showed 99-99.67% homology with the strain wnv/leiv-vlgoo-27924 o ...200617094656
evaluation of potential west nile virus vectors in volgograd region, russia, 2003 (diptera: culicidae): species composition, bloodmeal host utilization, and virus infection rates of mosquitoes.potential west nile virus (family flaviviridae, genus flavivirus, wnv) vectors were assessed during 2003 at indoor and outdoor collection sites in urban volgograd, russia, and in three nearby towns and surrounding rural areas. in total, 9,182 female mosquitoes comprising 13 species in six genera were collected. relative abundance and bloodmeal host utilization differed temporarily and spatially. during june and july in volgograd, aedes vexans (meigen) (85.4%) and culex p. pipiens l. (7.6%) were ...200616739415
[assessment of epidemic manifestations of the west nile fever in the volgograd region depending on the climatic conditions].results of the analysis of the increase in the incidence of epidemic of the west nile fever and climate conditions in the volgograd region were presented. certain seasonal periods and threshold values of temperature and humidity statistically associated with the epidemic rise were identified. the discussion of the probable mechanisms of indirect effects of atmospheric heat on the elements of the epidemic process was carried out.201425929037
[pathomorphology of west nile fever].8 patients, aged from 61 to 70 years, died from serous meningoencephalitis during the outburst of the west nile fever in volgograd in 1999-2001, were studied morphologically. serous meningoencephalitis with necrotic vasculitis is a characteristic feature of this fever with degenerative changes and destructive foci in the brain. proteinic and fatty inflammatory and lymphomacrophageal reactions were observed in the liver, serous productive myocarditis and cardiomyocytic necrosis dystrophy. desquam ...200415575379
[detection of the west nile virus and its genetic typing in ixodid ticks (parasitiformes: ixodidae) in tomsk city and its suburbs].four tick species, ixodes persulcatus, i. pavlovskyi, i. trianguliceps, and dermacentor reticulatus, were found in tomsk and its suburbs in 2006. the species i. pavlovskyi was found to be dominant in the localities situated in tomsk city, and i. persulcatus was dominant in its suburbs. viral rna and viral antigen of the west nile virus (wnv) were detected in the ticks i. pavlovskyi and i. persulcatus collected in the city and its suburbs by the rt pcr method and enzyme immunoassay with monoclona ...200818727366
[detection of west nile virus in birds in the territories of baraba and kulunda lowlands (west siberian migration way) during summer-autumn of 2002].west nile virus (wnv) was discovered in 3 species of birds collected in summer-autumn, 2002, in the south of western siberia. wnv was identified by elisa and rt-pcr. three of 5 dead rooks (corvus frugilegus), which were found in the territory of the kulunda plain, were wnv-infected. wnv rna was detected in 2% of samples of internal organs of aquatics birds, i.e. teal (anas crecca) and garganey (anas querquedula), caught in the chany lake (baraba plain). nucleotide sequencing of the 300-472 aa fr ...200415188656
[specific antibodies in convalescents after west nile fever].blood serum samples were obtained from patients with west nile fever (wnf) from the city of volgograd and its region within the disease weeks 1-3 (july-october 1999), month 6-7 (may_june 2000) and month 12-18 (may-june 2001) after the disease onset. in the sera of 40 patients examined 6-7 months after the disease onset igm-antibodies to wnf virus were absent (dilution 1:50), igg ones were detected in 35 patients (87.5%). 18 months after the disease igg-antibodies were identified in 23 of 26 conv ...200212508675
west nile encephalitis in russia 1999-2001: were we ready? are we ready?in 1963-1993, several strains of west nile virus (wnv) were isolated from ticks, birds, and mosquitoes in the southern area of european russia and western siberia. in the same regions, anti-wnv antibody was found in 0.4-8% of healthy adult donors. sporadic human clinical cases were observed in the delta of the volga river. in spite of this, wnv infection was not considered by the health authorities as a potentially emerging infection, and the large wnv outbreak in southern russia, started in lat ...200111797768
[mosquito complex (diptera, culicidae) in a west nile fever focus in the volgograd region. ii. host-feeding patterns of mosquitoes in different habitats].host preference of the mosquitoes collected in the urban and rural habitats of volgograd and its suburbs was studied by the precipitation reaction test. human and avian blood was detected in cx. pipiens, cx. modestus, ae. vexans, ae. behningi, ae. caspius, ae. sticticus, and females of the anopheles maculipennis. the proportion of the mosquitoes fed on birds was similar in the urban and rural biotopes whereas that of the mosquitoes feeding on humans was significantly higher in volgograd than in ...200717657957
[the influence of weather conditions on the epidemiology of vector-borne diseases by the example of west nile fever in russia].climate changes must influence the incidence of vector-borne infections, but their effects cannot be revealed due to lack of long-term observations. the impact of short-term weather changes may be used as a model. in russia the biggest numbers of clinical cases of mosquito-borne west nile infection were registered in 1999 in volgograd and astrakhan regions. the analysis of climatic dataset since 1900 shows that 1999 was the hottest year in volgograd in the 20th century due to a very mild winter ...200616544901
[isolation of the west nile fever virus from human patients during an epidemic outbreak in the volgograd and astrakhan regions].two strains of west nile virus leiv 27889 vig and ast 986 were isolated from the brain of a dead subject and from the blood of a patient, respectively, during an outbreak of serous meningitis and meningoencephalitis in july-september, 1999, in the volgograd region, krasnodar territory, and astrakhan region. these strains reacted with convalescent sera in hemagglutination inhibition test, which proves their etiological role in this outbreak.200010867987
[the study of mosquito fauna (diptera, culicidae) in volgograd city in light of the outbreak of west nile fever in volgograd region, 1999].mosquito sampling was carried out in volgograd city and its vicinity in august 2001 and 2002. in total 16,000 individuals belonging to 6 genera and 12 species were collected. nine species were anthropophilic. culex modestus and aedes vexans dominated in all outdoor samples collected in volgograd city. in addition to these species, coquillettidia richiardii and ae. caspius were abundant in the vicinity of volgograd. autogenous cx. popiens dominated among six species sampled indoors in volgograd c ...200415272818
[epidemic outbreak of meningitis and meningoencephalitis, caused by west nile virus, in the krasnodar territory and volgograd region (preliminary report)].sera from 102 inpatients from the volgograd region (64) and krasnodar region (38) were tested for antibodies to west nile (wn) virus in hemagglutination inhibition (hi) test and for igm and igg antibodies in enzyme immunoassay (eia). diseases etiologically associated with wn virus were diagnosed in 81 patients: in 50 out of 64 in the volgograd region and in 31 out of 38 in the krasnodar region, which makes 79.4%. specificity of antibodies to wn virus was confirmed in hi and eia with wn antigens, ...200010695043
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