usefulness of a combination of pulsed-field gel electrophoresis and enrichment culture in laboratory investigation of a foodborne outbreak due to clostridium perfringens. | clostridium perfringens is ubiquitous in nature and normally detectable in human stools. therefore, it is difficult to perform specific microbiologic diagnosis in foodborne outbreaks, particularly when only a few cultures are detected from fecal specimens. usually, it has been necessary to detect over 10(6) spores/g of fecal sample as a diagnostic criterion of diarrhea due to c. perfringens. a relatively large foodborne outbreak occurred in osaka city, japan in october 2001. although c. perfring ... | 2003 | 14596965 |