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usefulness of a combination of pulsed-field gel electrophoresis and enrichment culture in laboratory investigation of a foodborne outbreak due to clostridium perfringens.clostridium perfringens is ubiquitous in nature and normally detectable in human stools. therefore, it is difficult to perform specific microbiologic diagnosis in foodborne outbreaks, particularly when only a few cultures are detected from fecal specimens. usually, it has been necessary to detect over 10(6) spores/g of fecal sample as a diagnostic criterion of diarrhea due to c. perfringens. a relatively large foodborne outbreak occurred in osaka city, japan in october 2001. although c. perfring ...200314596965
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