| genetic variation and pathogenicity of highly virulent porcine reproductive and respiratory syndrome virus emerging in china. | a highly pathogenic swine disease designated as 'porcine high fever disease (phfd)' appeared recently in china. porcine reproductive and respiratory syndrome virus (prrsv) was identified as an agent associated with phfd, and two discontiguous sequence deletions were identified as a genetic marker in the nsp2 region of the viral genome. to examine phfd in shandong province, a total of 10 prrsv isolates were recovered from pig herds that had never been vaccinated for prrs. sequence analysis of ope ... | 2009 | 19672556 |
| novel nsp2 deletion based on molecular epidemiology and evolution of porcine reproductive and respiratory syndrome virus in shandong province from 2013 to 2014. | porcine reproductive and respiratory syndrome (prrs) is an economically important swine disease affecting swine worldwide. in this study, a total of 385 samples were collected from shandong pig farms during 2013 and 2014, when pigs were not inoculated with any vaccine. results indicated that, out of 385 samples, 47 (12.21%) were prrsv-rna-positive. the gene sequence analysis of 12 orf5, 12 orf7, and 8 nsp2 of these samples was used to determine the molecular epidemiology of prrsv in different pa ... | 2015 | 25958135 |
| sequence analysis of the nsp2, orf5, and orf7 genes of 11 prrs virus isolates from china. | during a tracing investigation of blue ear disease in china conducted from january to november 2008, 11 porcine reproductive and respiratory syndrome virus (prrsv) isolates were collected from eight provinces including liaoning, jilin, hebei, shandong, henan, shanghai, zhejiang, and guangxi. the complete gene sequences of the nsp2, orf5, and orf7 genes from these 11 prrsv isolates were amplified, cloned and detected by rt-pcr and then compared with the published sequences of other strains. the r ... | 2012 | 22644762 |