| development of an immunochromatographic strip for serological diagnosis of porcine hemagglutinating encephalomyelitis virus. | an immunochromatographic strip was developed for the detection of an antibody against porcine hemagglutinating encephalomyelitis virus (phev). colloidal gold-labeled rabbit anti-pig immunoglobulin g (igg) was used as the detection reagent, and the phev recombinant antigens and goat anti-rabbit igg were coated on the prototype strip and the control lines, respectively. the immunochromatographic strip was capable of specifically detecting phev antibodies in serum with a hemagglutination inhibition ... | 2011 | 21398449 |
| vomiting and wasting disease associated with hemagglutinating encephalomyelitis viruses infection in piglets in jilin, china. | one coronavirus strain was isolated from brain tissues of ten piglets with evident clinical manifestations of vomiting, diarrhea and dyskinesia in jilin province in china. antigenic and genomic characterizations of the virus (isolate phev-jlsp09) were based on multiplex pcr and negative staining electron microscopy and sequence analysis of the hemagglutinin-esterase (he) gene. these piglets were diagnosed with porcine hemagglutinating encephalomyelitis virus (phev).necropsy was performed on the ... | 2011 | 21418610 |
| identification and genetic characterization of porcine hemagglutinating encephalomyelitis virus from domestic piglets in china. | in this study, we investigated an acute outbreak of porcine hemagglutinating encephalomyelitis on a farm of 127 pigs in jilin province, china. porcine hemagglutinating encephalomyelitis virus (phev) was detected in suckling and weaning pigs by rt-pcr assays. coronavirus-like particles were observed by electron microscopy. the virus isolate was designated phev-jt06. the clinical signs, nervous symptoms and positive labeling of neurons in the cerebral cortex with an immunohistochemical stain in ph ... | 2014 | 24756345 |