| the paraphyletic composition of leishmania donovani zymodeme mon-37 revealed by multilocus microsatellite typing. | multilocus microsatellite typing (mlmt) was employed to compare strains of leishmania donovani belonging to the mon-37 zymodeme (mon-37 strains) from cyprus and israel to mon-37 strains from the indian subcontinent, the middle east, china and east africa as well as strains of other zymodemes. the mlmt data were processed with a distance-based method for construction of phylogenetic trees, factorial correspondence analysis and a bayesian model-based clustering algorithm. all three approaches assi ... | 2009 | 19376262 |
| development of an immunochromatographic test for diagnosis of visceral leishmaniasis based on detection of a circulating antigen. | visceral leishmaniasis (vl) is a life-threatening disease caused by protozoan parasites of the leishmania donovani complex. early case detection followed by adequate treatment is essential to the control of vl. however, the available diagnostic tests are either invasive and require considerable expertise (parasitological demonstration of the parasite in tissue smears) or unable to distinguish between past and active infection (serological methods). therefore, we aimed to develop a lateral flow a ... | 2015 | 26125560 |
| phylogenic analysis of chinese leishmania isolates based on small subunit ribosomal rna (ssu rrna) and 7 spliced leader rna (7sl rna). | the leishmaniases are zoonotic diseases caused by protozoan parasites of the genus leishmania. leishmaniases are still endemic in china, especially in the west and northwest froniter regions. to revalue the preliminary phylogenetic results of chinese leishmania isolates, we amplified partial fragment of small subunit ribosomal rna (ssu rrna) and 7 spliced leader rna (7sl rna), then tested the phylogenetic relationships among chinese leishmania isolates and their relatives by analyzing ssu rrna g ... | 2012 | 22807046 |
| molecular detection, identification and phylogenetic inference of leishmania spp. in some desert lizards from northwest china by using internal transcribed spacer 1 (its1) sequences. | leishmaniasis caused by leishmania is still endemic in northwest china. it has been thought that reptiles could be a reservoir for mammalian leishmaniasis. however, data are still scarce on natural infection of lizards with leishmania spp. in china. the present study deals with detection, identification and phylogenetic inference of leishmania parasites at species and intraspecies levels isolated from six desert lizard species from 10 geographical locations in northwest china using amplification ... | 2016 | 27338182 |
| analysis of kinetoplast cytochrome b gene of 16 leishmania isolates from different foci of china: different species of leishmania in china and their phylogenetic inference. | leishmania species belong to the family trypanosomatidae and cause leishmaniasis, a geographically widespread disease that infects humans and other vertebrates. this disease remains endemic in china. due to the large geographic area and complex ecological environment, the taxonomic position and phylogenetic relationship of chinese leishmania isolates remain uncertain. a recent internal transcribed spacer 1 and cytochrome oxidase ii phylogeny of chinese leishmania isolates has challenged some asp ... | 2013 | 23383990 |
| [acquisition and analysis of lipophosphoglycan 1 gene and the noncoding region of leishmania donovani isolates from hilly foci of china]. | a fragment about 2.2 kb located at upstream of lipophosphoglycan 1 (lpg1) gene of leishmania donovani isolate from hilly foci of china was obtained by pcr. the nucleotide sequence of the fragment was determined by sequencing. the sequence of the lpg1 gene and its downstream fragment (about 2 kb) was determined by using genome walking method. the above two fragment splicing result showed that the nucleotide sequence of the lpg1 gene with the noncoding region was 4 121 bp (genbank accession number ... | 2010 | 21500538 |
| molecular characterization of leishamania isolates from china by inter-simple sequence repeat polymerase chain reaction. | leishmania has distinct epidemiological and biological characteristics and causes a variety of clinical symptoms. to understand the genetic diversity and the phylogenetic relationships among leishmania isolates from china, 29 leishmania isolates from different geographic origins, vectors, and hosts were analyzed using 21 inter-simple sequence repeat polymerase chain reaction (issr-pcr) primers. a total of 864 polymorphic bands were obtained. according to the results of the neighbor-joining phylo ... | 2010 | 20237800 |
| [cloning and sequence analysis of its gene of leishmania donovani isolates from different epidemic foci in china]. | this study was directed to determine the nucleotide sequence of the its (internal transcribed spacer) gene of leishmania donovani isolates from desert foci (l. d xj771), hill foci (l. d sc10) and plain foci (l. d sd2), and to find out the differences of the sequences of its gene among these three isolates. the specific its fragments from nuclear dna of three leishmania isolates were amplified by pcr, cloned into pmd18-t vector, and then sequenced by the dideoxy chain termination method. sequence ... | 2005 | 16013255 |