| review of beet pseudoyellows virus genome structure built the consensus genome organization of cucumber strains and highlighted the unique feature of strawberry strain. | the complete nucleotide sequences of beet pseudoyellows virus (bpyv)-mi (cucumber isolate; matsuyama, idai) genomic rnas 1 and 2 were determined and compared with the previously sequenced japanese cucumber strain (bpyv-jc) and a strawberry strain (bpyv-s). the rna 2 of bpyv-mi showed 99 % nucleotide sequence identity with both bpyv-jc and -s having highly conserved eight orfs. in contrast, the rna1 of bpyv-mi showed sequence identities of 98 and 86 % with bpyv-jc and -s, respectively. phylogenet ... | 2016 | 27459886 |
| whitefly-transmitted criniviruses of cucurbits: current status and future prospects. | in the past decade, crinviruses have gained interest due to their rapid widespread and destructive nature for cucurbit cultivation. several members of the genus crinivirus are considered emerging viruses. currently, four criniviruses: beet pseudo-yellows virus, cucurbit chlorotic yellows virus, cucurbit yellow stunting disorder virus and lettuce infectious yellows virus have been reported to infect field- or greenhouse- grown cucurbits. apart from their cucurbit hosts, criniviruses infect other ... | 2014 | 24426308 |
| biological and molecular characterization of a korean isolate of cucurbit aphid-borne yellows virus infecting cucumis species in korea. | surveys of yellowing viruses in plastic tunnels and in open field crops of melon (cucumis melo cultivar catalupo), oriental melon (c. melo cultivar oriental melon), and cucumber (c. sativus) were carried out in two melon-growing areas in 2014, korea. severe yellowing symptoms on older leaves of melon and chlorotic spots on younger leaves of melon were observed in the plastic tunnels. the symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thi ... | 2015 | 26673519 |
| two crinivirus-specific proteins of lettuce infectious yellows virus (liyv), p26 and p9, are self-interacting. | interactions of lettuce infectious yellows virus (liyv)-encoded proteins were tested by yeast-two-hybrid (y2h) assays. liyv-encoded p34, hsp70h, p59, cp, cpm, and p26 were tested in all possible pairwise combinations. interaction was detected only for the p26-p26 combination. p26 self-interaction domains were mapped using a series of n- and c-terminal truncations. orthologous p26 proteins from the criniviruses beet pseudoyellows virus (bpyv), cucurbit yellow stunting disorder virus (cysdv), and ... | 2009 | 19665507 |
| geographic distribution and molecular variation of isolates of three whitefly-borne closteroviruses of cucurbits: lettuce infectious yellows virus, cucurbit yellow stunting disorder virus, and beet pseudo-yellows virus. | abstract the geographic incidence and molecular variation of three whitefly-borne closteroviruses (lettuce infectious yellows virus [liyv], cucurbit yellow stunting disorder virus [cysdv], and beet pseudo-yellows virus [bpyv]) were studied in cucurbits collected from several distinct geographic locations. of 498 samples analyzed, none were found to be infected by liyv. sixty-nine samples collected in the middle east and mediterranean europe were found infected by cysdv, and twelve samples from c ... | 1999 | 18944685 |
| nucleotide sequence of blackberry yellow vein associated virus, a novel member of the closteroviridae. | the complete nucleotide sequence of a novel member of the genus crinivirus (family closteroviridae), isolated from blackberry and tentatively named blackberry yellow vein associated virus, was determined. the virus possesses a bipartite genome. rna 1 is 7,801 nucleotides in length and papain-like protease, methyltransferase, rna helicase and rna-dependent rna polymerase motifs have been identified in the proteins coded for by this molecule. the polymerase is probably expressed via a +1 ribosomal ... | 2006 | 16332399 |
| complete nucleotide sequence of a strawberry isolate of beet pseudoyellows virus. | in our effort to identify the causal agent(s) of strawberry pallidosis we found a single pallidosis positive plant that did not give any amplicons after rt-pcr using primer sets representing multiple regions of strawberry pallidosis associated virus (spav) genome and failed to react with antibodies directed against the recombinant coat protein (cp) of spav. dsrna extracted from this plant showed a similar pattern to that of spav indicating that another crinivirus may infect strawberry. sequence ... | 2004 | 15266105 |
| identification and sequence analysis of potato yellow vein virus capsid protein minor gene. | potato yellow vein virus (pyvv) is a whitefly-transmitted (trialeurodes vaporariorum) closterovirus (wtc) with an as yet unidentified genome composition. pyvv dsrna preparations consist of three high molecular weight dsrna species (dsrnas 1, 2 and 3) 8.0, 5.5 and 4.0 kbp in size respectively, as well as two low molecular weight dsrna species of 2.0 and 1.8 kbp (denoted x and y). the pyvv capsid protein minor (cpm) gene was identified on the dsrna 3 species, and was subsequently cloned and sequen ... | 2002 | 12881642 |
| beet pseudo-yellows virus is an authentic closterovirus. | we have previously used rt-pcr to amplify a 251 base pair fragment diagnostic for beet pseudo-yellows virus (bpyv) infection from several bpyv infected samples from europe and the usa. this has now been shown by sequencing to represent a fragment of a heat shock protein (hsp)70 gene. sequence analysis of the fragment revealed that it is closely similar to the c-terminal sequence of the hsp70 homologue gene of lettuce infectious yellows virus, a definitive closterovirus, and also has obvious sequ ... | 1996 | 8972572 |
| dsrna cloning and diagnosis of beet pseudo-yellows virus by pcr and nucleic acid hybridization. | dsrna has been extracted from beet pseudo-yellows virus infected cucumber plants, purified to homogeneity, and cdna clones to it produced. the clones are of insufficient sensitivity to detect infection-specific rna in dot and northern blots of crude nucleic acid extracts. however, a knowledge of the sequence of these clones has been used to synthesize oligonucleotides that have been used for polymerase chain reaction amplification of specific sequences from both purified dsrna and from infected ... | 1992 | 1526749 |
| nucleotide sequence, genome organization and phylogenetic analysis of strawberry pallidosis associated virus, a new member of the genus crinivirus. | the complete nucleotide sequence of strawberry pallidosis associated virus (spav), a newly identified member of the genus crinivirus, family closteroviridae has been determined. rna 1 is 8067 nucleotides long and encodes at least three open reading frames (orfs). the first orf (orf 1a) specifies a multifunctional protein that has papain-like proteinase, methyltransferase and rna helicase domains. the rna-dependent-rna polymerase is encoded in orf 1b and is probably expressed by a +1 ribosomal fr ... | 2005 | 15503221 |