| a metagenomics and case-control study to identify viruses associated with bovine respiratory disease. | bovine respiratory disease (brd) is a common health problem for both dairy and beef cattle, resulting in significant economic loses. in order to identify viruses associated with brd, we used a metagenomics approach to enrich and sequence viral nucleic acids in the nasal swabs of 50 young dairy cattle with symptoms of brd. following deep sequencing, de novo assembly, and translated protein sequence similarity searches, numerous known and previously uncharacterized viruses were identified. bovine ... | 2015 | 25740998 |
| comparison of nested pcr and qpcr for the detection and quantitation of bohv6 dna. | nested pcr and qpcr (quantitative pcr) tests based on glycoprotein b (gb) gene were designed for detecting bovine herpesvirus 6 (bohv6) in bovine whole blood samples and wild ruminant blood clots (deer and roe-deer). this virus, commonly known as blhv (bovine lymphotropic herpesvirus) belongs to the herpesviridae family, subfamily gammaherpesvirinae and macavirus genus. dna isolated from 92 dairy cow blood samples and 69 wild ruminant clots were examined for the presence of bohv6 using nested pc ... | 2013 | 23954301 |
| multicentric lymphoma in buffaloes in the amazon region, brazil. | the presence of lymphoma in buffaloes was first reported in india in the 1960s. the disease is similar to enzootic bovine leucosis (ebl) caused by bovine leukemia virus (blv) in cattle; however, according to our results and those of other studies, the etiology of these lymphomas in buffalo do not appear to be associated with blv. the objectives of this study are to describe four cases of the disease in buffaloes belonging to the same herd in the amazon region of brazil and to perform a clinical- ... | 2016 | 27765074 |
| bovine herpesvirus 6 in buffaloes (bubalus bulalis) from the amazon region, brazil. | this study presents the first description of bovine herpesvirus 6 (bohv-6) that was isolated from buffaloes of amazon region in brazil. phylogenetic analysis showed that the bohv-6 brazilian strains clustered with the sequence of bohv-6 from elsewhere available at the genbank. it was observed in some buffaloes with lymphoproliferative disease in one herd, thus the animals were also tested for bovine leukemia virus (blv), which has been associated to lymphoma in bovines. all animals were negative ... | 2015 | 25427628 |
| exploring the virome of cattle with non-suppurative encephalitis of unknown etiology by metagenomics. | non-suppurative encephalitis is one of the most frequent pathological diagnosis in cattle with neurological disease, but there is a gap in the knowledge on disease-associated pathogens. in order to identify viruses that are associated with non-suppurative encephalitis in cattle, we used a viral metagenomics approach on a sample set of 16 neurologically-diseased cows. we detected six virus candidates: parainfluenza virus 5 (piv-5), bovine astrovirus ch13/neuros1 (boastv-ch13/neuros1), bovine poly ... | 2016 | 26994586 |
| novel gammaherpesvirus functions encoded by bovine herpesvirus 6 (bovine lymphotropic virus). | the genus macavirus of the subfamily gammaherpesvirinae includes viruses that infect lymphoid cells of domestic and wild ruminants and swine, causing asymptomatic latent infections in reservoir hosts. here, we describe the genome of bovine herpesvirus 6 (bohv-6), a macavirus ubiquitous in healthy cattle populations. the bohv-6 genome exhibited architecture conserved in macaviruses, including a repetitive h-dna region and unique 141 kbp l-dna region predicted to encode 77 genes. bohv-6 encoded, i ... | 2014 | 24836671 |
| neuropathological survey reveals underestimation of the prevalence of neuroinfectious diseases in cattle in switzerland. | neuroinfectious diseases in livestock represent a severe threat to animal health, but their prevalence is not well documented and the etiology of disease often remains unidentified. the aims of this study were to generate baseline data on the prevalence of neuroinfectious diseases in cattle in switzerland by neuropathological survey, and to identify disease-associated pathogens. the survey was performed over a 1-year period using a representative number of brainstem samples (n=1816) from fallen ... | 2017 | 28888628 |