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tick-borne rickettsioses in pune district, maharashtra, india.an extensive study on tick-borne rickettsioses in the pune district of maharashtra revealed that indian tick typhus exists as a zoonosis, which only occasionally causes disease in man. by sero-conversion in guinea pigs, presumptive isolates of rickettsia conori and coxiella burnetii were recovered from 4 of the 11 species of ticks examined. boophilus microplus and rhipicephalus haemaphysalis were found to be harbouring r. conori whereas c. burnetii was isolated from haemaphysalis intermedia and ...19846500861
[serological study of rickettsia infections in niamey, niger].rickettsioses is a possible alternative to presumptive diagnosis of malaria. a serologic study was carried out in 1994 to determine the prevalence of rickettsioses in children under 5 years of age from three different areas of niamey, niger. indirect immunofluorescent assays using the micromethod were performed with antigens for rickettsia conori, rickettsia mooseri, and coxiella burneti. results were read from a positive threshold of 1/160 up to 1/640. out of a randomized population of 177 chil ...19979304008
new approaches to detection and identification of rickettsia africae and ehrlichia ruminantium in amblyomma variegatum (acari: ixodidae) ticks from the caribbean.imported from africa in the 1700s and despite frequent modern eradication efforts, amblyomma variegatum (f.) spread through the caribbean by cattle transport, small ruminants, and migrating birds. a. variegatum is a vector for rickettsia africae, the causative agent of african tick bite fever, and ehrlichia ruminantium, the causative agent of heartwater. we examined 95 a. variegatum and six rhipicephalus (boophilus) microplus (canestrini) collected from cattle at an abattoir in antigua. engorged ...200919645301
tick-borne bacteria in free-living jaguars (panthera onca) in pantanal, brazil.abstract tick-borne bacteria were investigated in 10 free-living jaguars and their ticks in the pantanal biome, brazil. jaguar sera were tested by indirect fluorescent antibody assays using rickettsia rickettsii, rickettsia parkeri, rickettsia amblyommii, rickettsia rhipicephali, rickettsia felis, rickettsia bellii, ehrlichia canis, and coxiella burnetii as crude antigens. all 10 jaguar sera reacted (titer ≥64) to at least one rickettsia species; 4 and 3 sera reacted with e. canis and c. burneti ...201121612532
influence of the biotope on the tick infestation of cattle and on the tick-borne pathogen repertoire of cattle ticks in ethiopia.the majority of vector-borne infections occur in the tropics, including africa, but molecular eco-epidemiological studies are seldom reported from these regions. in particular, most previously published data on ticks in ethiopia focus on species distribution, and only a few molecular studies on the occurrence of tick-borne pathogens or on ecological factors influencing these. the present study was undertaken to evaluate, if ticks collected from cattle in different ethiopian biotopes harbour (had ...201425248165
molecular detection of rickettsia, anaplasma, coxiella and francisella bacteria in ticks collected from artiodactyla in thailand.a total of 79 ticks collected from sambar deer (cervus unicolor), barking deer (muntiacus muntjak) and wild boar (sus scrofa) were examined by pcr for the presence of rickettsia, anaplasma, coxiella, and francisella bacteria. of the 79 ticks, 13% tested positive for rickettsia, 15% tested positive for anaplasma, 4% tested positive for coxiella, and 3% tested positive for francisella. interestingly, triple infection with anaplasma, rickettsia and francisella was determined in a dermacentor auratu ...201626934997
molecular epidemiological surveillance to assess emergence and re-emergence of tick-borne infections in tick samples from china evaluated by nested pcrs.an investigation was performed to detect eight pathogens in ticks collected from grass tips or animals in the southern, central and northeast regions of china. dna samples extracted from ticks were collected from ten different locations in eight provinces of china and subjected to screening for tick-borne pathogens, including borrelia burgdorferi sensu lato, ehrlichia spp., rickettsia spp., babesia/theileria spp., ehrlichia ruminantium, coxiella burnetii, and francisella tularensis, using nested ...201626943995
coxiella symbionts are widespread into hard ticks.ticks are blood-feeding arthropods and can harbor several bacteria, including the worldwide zoonotic disease q-fever agent coxiella burnetii. recent studies have reported a distinct group of coxiella mostly associated with ixodidae ticks, including the primary endosymbionts of amblyomma americanum. in the present work, a screening for coxiella infection was performed by 16s ribosomal dna (rdna) gene analyses in 293 tick samples of 15 different species sampled worldwide, including brazil, colombi ...201627595990
seroepidemiologic survey in thailand of coxiella burnetii infection in cattle and chickens and presence in ticks attached to dairy cattle.a seroepidemiologic survey of coxiella burnetii in cattle and chickens in thailand was carried out using indirect fluorescent antibody test. nine of the 130 serum samples from cattle were positive for antibodies against c. burnetii, with antibody titers ranging from 32 to 64. only one of 113 serum samples from chickens was seropositive, with antibody titer of 16. no c. burnetii-specific dna was detected using restriction fragment length polymorphism-nested pcr in spleens of cattle and chickens. ...201425417520
molecular and maldi-tof identification of ticks and tick-associated bacteria in mali.ticks are considered the second vector of human and animal diseases after mosquitoes. therefore, identification of ticks and associated pathogens is an important step in the management of these vectors. in recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) has been reported as a promising method for the identification of arthropods including ticks. the objective of this study was to improve the conditions for the preparation of tick samples ...201728742123
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