predation of cryptosporidium oocysts by protozoa and rotifers: implications for water quality and public health.predation by free-living protozoa and rotifers was investigated as a possible mechanism for the removal of cryptosporidium parvum oocysts in aquatic ecosystems including wastewater treatment plants. free-living ciliated protozoa (stylonychia mytilus, paramecium caudatum and an unidentified wastewater wetland ciliate), an amoeba (acanthamoeba culbertsoni) and rotifers, all commonly found in aquatic ecosystems, were exposed to varying doses of c. parvum oocysts. all organisms investigated ingested ...200312639009
immunity to acanthamoeba culbertsoni: experimental studies with acanthamoeba and control antigen preparations.mice were intranasally immunized with living and killed acanthamoeba as well as culture supernatant, living toxoplasma and bcg, and challenged with pathogenic a. culbertsoni strain a-1. the best protection was achieved with killed amoebae of the same strain. a high rate of mortality was observed in immunized mice treated with immunosuppressives and challenged with a. culbertsoni a-1. the significance of the results in relation to environmental human infection is discussed.19873449996
activation of the alternative pathway of complement by acanthamoeba culbertsoni.normal human serum (nhs) contained an amoebicidal property for acanthamoeba culbertsoni. killing was quantitated by measuring the ability of the amoebae to undergo cell division subsequent to exposure to nhs, and also by microscopical examination. plasma membrane disruption and extrusion of intracellular components occurred within 5-10 min following exposure to nhs. adsorption of specific antibody did not remove the amoebicidal activity while heating serum at 56 degrees c/30 min or treatment wit ...19836418422
retrospective identification of acanthamoeba culbertsoni in a case of amoebic meningoencephalitis.acanthamoeba culbertsoni was identified retrospectively in a case of amoebic meningoencephalitis, previously reported by jager and stamm (lancet, 2, 1343, 1972). this is the second report of this species causing secondary infection in man. positive results were obtained only with anti-a. culbertsoni sera when the brain sections were stained by the indirect immunofluorescence antibody test with various antisera produced against different acanthamoeba species. antiserum raised against purified pla ...1978357454
identification of antigens of pathogenic free-living amoebae by protein immunoblotting with rabbit immune and human sera.prominent antigens of pathogenic and nonpathogenic free-living amoebae were identified by using polyclonal rabbit immune sera in immunoblot assays. the intent was to determine if prominent epitopes identified with rabbit immune sera could also be recognized by human sera. with rabbit sera, the development of immunoreactive bands was restricted to molecular masses of greater than 18.5 kda for naegleria, hartmannella, and vahlkampfia antigens. two or more broad bands of less than 18.5 kda were pro ...19948556491
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