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babesia bigemina infection in yak (poephagus grunniens l.): molecular detection and characterization.yaks contribute significantly in the himalayan high land economy. specific information on prevalence of babesiosis in yaks is lacking. a fast and reliable pcr assay targeting babesia bigemina small subunit ribosomal rna sequence (ss rrna) was laboratory standardized for molecular detection of b. bigemina in yaks. restriction digestion of the pcr amplified 675 bp target sequence with vsp i confirmed the prevalent species of babesia as b. bigemina. nucleotide sequencing and phylogenetic analysis o ...201323298564
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